We narrowed to 45,509 results for: cha
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Plasmid#192843PurposePlasmid to produce ISH probe for mMyh3 gene with CDS sequenceDepositorInsertmyosin heavy chain 3 (Myh3 Mouse)
Available SinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-mMyh3UTR
Plasmid#192844PurposePlasmid to produce ISH probe for mMyh3 gene with UTR sequenceDepositorInsertmyosin heavy chain 3 (Myh3 Mouse)
Available SinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-mMyh4CDS
Plasmid#192845PurposePlasmid to produce ISH probe for mMyh4 gene with CDS sequenceDepositorInsertmyosin heavy chain 4 (Myh4 Mouse)
Available SinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-mMyh4UTR
Plasmid#192846PurposePlasmid to produce ISH probe for mMyh4 gene with UTR sequenceDepositorInsertmyosin heavy chain 4 (Myh4 Mouse)
Available SinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-mMyh6CDS
Plasmid#192847PurposePlasmid to produce ISH probe for mMyh6 gene with CDS sequenceDepositorInsertmyosin heavy chain 6 (Myh6 Mouse)
Available SinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-mMyh6UTR
Plasmid#192848PurposePlasmid to produce ISH probe for mMyh6 gene with UTR sequenceDepositorInsertmyosin heavy chain 6 (Myh6 Mouse)
Available SinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-mMyh8UTR
Plasmid#192852PurposePlasmid to produce ISH probe for mMyh8 gene with UTR sequenceDepositorInsertmyosin heavy chain 8 (Myh8 Mouse)
Available SinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC25A46-His (SB250)
Plasmid#227599PurposeInducible expression of His-tagged SLC25A46 in Pichia pastorisDepositorAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SLC25A46-P2A-Electra1 (pAH9)
Plasmid#227614PurposeOverexpression of SLC25A46 in mammalian cell linesDepositorAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57-CMV-MAAP-SL01V6
Plasmid#218249Purpose1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.DepositorInsertMembrane-Associated Accessory Protein Variant SL01V6 driven by CMV promoter
UseLentiviralPromoterCMVAvailable SinceOct. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57-CMV-MAAP-SL01V9
Plasmid#218250Purpose1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.DepositorInsertMembrane-Associated Accessory Protein Variant SL01V9 driven by CMV promoter
UseLentiviralPromoterCMVAvailable SinceOct. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57-CMV-MAAP-SL08V9
Plasmid#218251Purpose1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.DepositorInsertMembrane-Associated Accessory Protein Variant SL08V9 driven by CMV promoter
UseLentiviralPromoterCMVAvailable SinceOct. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57-CMV-MAAP-SL01
Plasmid#218248Purpose1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.DepositorInsertMembrane-Associated Accessory Protein Variant SL01 driven by CMV promoter
UseLentiviralPromoterCMVAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
PtetA-PBAD-PLacO3O1 200bp with pBELO_L6-PT3-T4_CAMR_TAN+
Plasmid#225649PurposetetA, araBAD and LacO3O1 promoters in tandem conformation with 200bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistanceDepositorInserttetR/tetA, araBAD and LacO3O1 in tandem conformation
TagsmCherryExpressionBacterialPromotertetA, araBAD and LacO3O1 promotersAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG-B2-ELP-HIS-ybbR
Plasmid#169134PurposeE. coli expression of SdrG N2, N3 and B2 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.DepositorInsertStaphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
Tags3xELP linker, 6x Histag, and ybbr tagExpressionBacterialAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Fgß-FIVAR-ELP-HIS-ybbR
Plasmid#169136PurposeE. coli expression of FIVAR domain containing Fgß tag, ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.DepositorInsertFIVAR (found in various architectures)
Tags3xELP linker, 6x Histag, Fgβ peptide, and ybbr ta…ExpressionBacterialAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-VHH-ddFLN4-HIS-ybbR
Plasmid#169137PurposeE. coli expression of VHH domain containing ybbR tag and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.DepositorInsertVHH
Tags6x His tag, ddFLN4 tag, and ybbr tagExpressionBacterialAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG-B1-ELP-HIS-ybbR
Plasmid#169133PurposeE. coli expression of SdrG N2, N3 and B1 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.DepositorInsertStaphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
Tags3xELP linker, 6x Histag, and ybbr tagExpressionBacterialAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:hTRAAK(Q258K)
Plasmid#224763PurposeIn vitro transcription for Oocyte expression of hTRAAK(Q258K)DepositorInsertPotassium channel subfamily member 4 (KCNK4 Human)
UseIn vitro transcription for oocyte expressionMutationQ258KPromoterT7Available SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1
Plasmid#224743PurposeIn vitro transcription for Oocyte expression of mTREK-1DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionPromoterT7Available SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:hTRAAK
Plasmid#224761PurposeIn vitro transcription for Oocyte expression of hTRAAKDepositorInsertPotassium channel subfamily member 4 (KCNK4 Human)
UseIn vitro transcription for oocyte expressionPromoterT7Available SinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:hTRAAK(S118C)
Plasmid#224762PurposeIn vitro transcription for Oocyte expression of hTRAAK(S118C)DepositorInsertPotassium channel subfamily member 4 (KCNK4 Human)
UseIn vitro transcription for oocyte expressionMutationS118CPromoterT7Available SinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTRAAK
Plasmid#224765PurposeIn vitro transcription for Oocyte expression of mTRAAKDepositorInsertPotassium channel subfamily member 4 (Kcnk4 Mouse)
UseIn vitro transcription for oocyte expressionPromoterT7Available SinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Pt NaV1.4-N1600T
Plasmid#200289PurposeExpression in mammalian cells or oocytesDepositorInsertPhyllobates terriblis NaV1.4 – muscle channel
ExpressionMammalianMutationN1600TPromoterCMV and T7Available SinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Dt NaV1.4-N1600T
Plasmid#200308PurposeExpression in mammalian cells or oocytesDepositorInsertDendrobates tinctorius NaV1.4 – muscle channel
ExpressionMammalianMutationN1600TPromoterCMV and T7Available SinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEndo-I-OnuI-TSM
Plasmid#207954PurposeExpresses a fusion of the homing endonuclease I-OnuI with the thermosensitive L212P VMA1 intein. Endonuclease activity is restricted to lower temperatures.DepositorInsertThermosensitive fusion of I-OnuI and the L212P VMA1 intein.
UseSynthetic BiologyExpressionBacterialMutationLeucine 212 changed to proline, leucine 434 in fu…Available SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-tac-EcTyrRS-VSMA*-lpp-tRNA_CUA^EcTyr
Plasmid#218766Purposeencodes EcTyrRS-VSMA* and EcTyr-tRNA-TAGDepositorInsertEcTyrRS-VSMA*
ExpressionBacterialMutationY37V, D167G, D182S, F183M, L186A, D265RPromotertacIAvailable SinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-W371A I375A D384A Y387A-GFP
Plasmid#207025PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
TagsGFPExpressionYeastMutationW371A I375A D384A Y387APromoterpTEF1Available SinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCUP1-GFP-Pho8-406
Plasmid#207006PurposeIntegration of GFP-Pho8 as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Marker for yeast vacuolar membrane.DepositorInsertPHO8
TagsGFPExpressionYeastAvailable SinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pToxAmp1
Plasmid#218285PurposeAmplifying the gene copy number of heterologous genes (yEGFP and RelB) through ToxAmp (toxin-antitoxin-driven gene amplification) mechanismDepositorInsertHO(-253, -1)-pRPL8B>RelB>tPDC1-pTEF1>yEGFP>tURA3-ARS712-HO(-731, -264)
ExpressionYeastAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrA_F164R238
Plasmid#216230PurposeS. aureus USA300 GyrA mutant F164 R238 residues codon-optimized for E. coliDepositorInsertDNA gyrase
Tags6xHisExpressionBacterialMutationChanged arginine 238 to serine, phenylalanine 168…PromoterT7Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrB_A467
Plasmid#216234PurposeS. aureus USA300 GyrB mutant A467 residue codon-optimized for E. coliDepositorInsertDNA gyrase
Tags6xHisExpressionBacterialMutationChanged alanine 467 to valinePromoterT7Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrB_T522
Plasmid#216235PurposeS. aureus USA300 GyrB mutant T522 residue codon-optimized for E. coliDepositorInsertDNA gyrase
Tags6xHisExpressionBacterialMutationChanged threonine 522 to isoleucinePromoterT7Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCUP1-GFP-Osh1-406
Plasmid#207007PurposeIntegration of Osh1-GFP as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Yeast PMN pathway substrate.DepositorInsertOSH1
TagsGFPExpressionYeastAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
mApple-1xHp
Plasmid#214409PurposeLipid droplet and ER localization of 1xHp of Spastin fused to mAppleDepositorAvailable SinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt7 (136-403) L361C
Plasmid#213622PurposeBacterial expression of SUMO-tagged mouse syt7 (136-403) L361CDepositorAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-cPLA2 C2 domain (Y96C)
Plasmid#213633PurposeBacterial expression of SUMO-tagged human cPLA2 C2 domain (Y96C)DepositorInsertcPLA2 C2 domain (Y96C) (PLA2G4A Human)
TagsSUMOExpressionBacterialMutationY96C, C139A, C141SAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-P1K-3VSV-Atg8(P85L P86L)-MET15
Plasmid#207050PurposeExpression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).DepositorInsertATG8
Tags3xVSVExpressionYeastMutationP85L P86LAvailable SinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-P1K-3VSV-Atg8(T56A R65A T87A)-MET15
Plasmid#207051PurposeExpression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).DepositorInsertATG8
Tags3xVSVExpressionYeastMutationT56A R65A T87AAvailable SinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-P1K-3VSV-Atg8(R65A P85A P86A)-MET15
Plasmid#207052PurposeExpression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).DepositorInsertATG8
Tags3xVSVExpressionYeastMutationR65A P85A P86AAvailable SinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only