Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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pX330A_D10A-1x4

Plasmid

#58774

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pX330A_D10A-1x6

Plasmid

#58776

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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4A3.SU

Plasmid

#197675

Purpose

Expresses P1 S tail fibre and U chaperone

Depositor

Baojun Wang

Article

Huan et al J Mol Biol. 2022 Sep 15;434(21):167829.

Inserts

S
U

Expression

Bacterial

Promoter

Late S promoter, LPS and N/A

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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4A3.S'U'

Plasmid

#197676

Purpose

Expresses P1 S' tail fibre and U' chaperone

Depositor

Baojun Wang

Article

Huan et al J Mol Biol. 2022 Sep 15;434(21):167829.

Inserts

S'
U'

Expression

Bacterial

Promoter

Late S promoter, LPS and N/A

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pX330A_D10A-1x3

Plasmid

#58773

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pTSK573

Plasmid

#190881

Purpose

For C-terminal -Halo Tagging of a gene of interest with TRP1 selection marker in yeast S. cerevisiae

Depositor

Tatiana Karpova, Gunjan Mehta

Article

Podh et al STAR Protoc. 2022 Dec 16;3(4):101900. do

Insert

HaloTag

Use

Bacterial cloning vector

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-KRAS-G12D

Plasmid

#116423

Purpose

Lentiviral expression of KRAS G12D

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

KRAS (KRAS Human)

Use

Lentiviral

Mutation

G12D

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-KRAS

Plasmid

#116755

Purpose

Lentiviral expression of KRAS

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

KRAS (KRAS Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ERBB2-S310F

Plasmid

#116349

Purpose

Lentiviral expression of ERBB2 S310F

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERBB2 (ERBB2 Human)

Use

Lentiviral

Mutation

S310F

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-KRAS-G12V

Plasmid

#116424

Purpose

Lentiviral expression of KRAS G12V

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

KRAS (KRAS Human)

Use

Lentiviral

Mutation

G12V

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ERBB2-V842I

Plasmid

#116359

Purpose

Lentiviral expression of ERBB2 V842I

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERBB2 (ERBB2 Human)

Use

Lentiviral

Mutation

V842I

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ERBB2-D769H

Plasmid

#116327

Purpose

Lentiviral expression of ERBB2 D769H

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERBB2 (ERBB2 Human)

Use

Lentiviral

Mutation

D769H

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-KRAS-Q61R

Plasmid

#116425

Purpose

Lentiviral expression of KRAS Q61R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

KRAS (KRAS Human)

Use

Lentiviral

Mutation

Q61R

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pTL-DHDDS

Plasmid

#203177

Purpose

Centromeric yeast expression vector for hDHDDS

Depositor

William Sessa

Article

Galosi et al Brain. 2022 Mar 29;145(1):208-223. doi:

Insert

DHDDS (DHDDS Human)

Expression

Yeast

Promoter

RER2

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-ERBB2-G776V

Plasmid

#116333

Purpose

Lentiviral expression of ERBB2 G776V

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERBB2 (ERBB2 Human)

Use

Lentiviral

Mutation

G776V

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ERBB2-S310Y

Plasmid

#116350

Purpose

Lentiviral expression of ERBB2 S310Y

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERBB2 (ERBB2 Human)

Use

Lentiviral

Mutation

S310Y

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ERBB2-L755_T759delLRENT

Plasmid

#116339

Purpose

Lentiviral expression of ERBB2 L755_T759delLRENT

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERBB2 (ERBB2 Human)

Use

Lentiviral

Mutation

L755_T759delLRENT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-KRAS-A146V

Plasmid

#116422

Purpose

Lentiviral expression of KRAS A146V

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

KRAS (KRAS Human)

Use

Lentiviral

Mutation

A146V

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ERBB2-G776delinsVC

Plasmid

#116331

Purpose

Lentiviral expression of ERBB2 G776delinsVC

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERBB2 (ERBB2 Human)

Use

Lentiviral

Mutation

G776delinsVC

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ERBB2-R678Q

Plasmid

#116347

Purpose

Lentiviral expression of ERBB2 R678Q

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERBB2 (ERBB2 Human)

Use

Lentiviral

Mutation

R678Q

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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