We narrowed to 6,250 results for: nls

1. pMLS262

   Plasmid

   #73718

   Purpose

   C. elegans Pan-neuronal 2xNLS-FLP-D5 expression vector

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   Psnt-1::2xNLS-FLP-D5
   

   Expression

   Worm

   Mutation

   G5D

   Promoter

   Psnt-1

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMLS260

   Plasmid

   #73685

   Purpose

   Punc-17::2xNLS-FLP-D5 Expression vector

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   Punc-17::2xNLS-FLP-D5::let-858 3'-UTR
   

   Use

   Flp/frt

   Expression

   Worm

   Mutation

   G5D

   Promoter

   Punc-17

   Available Since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMLS360

   Plasmid

   #73687

   Purpose

   Punc-47::2xNLS-FLP-D5 Expression vector

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   Punc-47::2xNLS-FLP-D5::let-858 3'-UTR
   

   Use

   Flp/frt

   Expression

   Worm

   Promoter

   Punc-47

   Available Since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGC320

   Plasmid

   #19639

   Depositor

   Jane Hubbard

   Article

   Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

   Insert

   Gateway(R4-R3)

   Use

   Flp/frt, destination vector

   Tags

   4xNLS

   Expression

   Worm

   Available Since

   March 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGC93

   Plasmid

   #19628

   Depositor

   Jane Hubbard

   Article

   Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

   Insert

   Gateway(R1-R2)<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<1xNLS-lacZ::unc-54(3’)
   

   Use

   Flp/frt, destination vector

   Expression

   Worm

   Available Since

   March 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTK729

   Plasmid

   #114710

   Purpose

   Express NuMA(1-705+1700-2115ΔNLS)-RFP-Nano from Rosa 26 locus

   Depositor

   Tomomi Kiyomitsu

   Article

   Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

   Insert

   NuMA(1-705+1700-2115ΔNLS)-RFP-Nano
   

   Available Since

   April 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGC91

   Plasmid

   #19621

   Depositor

   Jane Hubbard

   Article

   Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

   Insert

   Prpl-28<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<1xNLS-lacZ::unc-54(3’)
   

   Use

   Flp/frt

   Expression

   Worm

   Available Since

   Feb. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGC200

   Plasmid

   #19623

   Depositor

   Jane Hubbard

   Article

   Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

   Insert

   Ppro-1<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<1xNLS-lacZ::unc-54(3’)
   

   Use

   Flp/frt

   Expression

   Worm

   Available Since

   Feb. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. iF67 PB-Zim3-XTEN-dCas9-mScarlet-Bsd-Neo-mAmetrine

   Plasmid

   #204732

   Purpose

   Constitutive CRISPRi by Zim3-dCas9

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Zim3-dCas9
   

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. iF51 PB-Zim3-dCas9-mScarlet-Hygro-Neo-SnapTag

    Plasmid

    #204725

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Zim3-dCas9
    

    Expression

    Mammalian

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-TO-NFIA.1-SOX9

    Plasmid

    #182310

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Inserts

    NFIA-T2A-SOX9 (NFIA, SOX9 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Piggybac

    Tags

    None and T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Promoter

    EF1a and TRE3G

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-ABE8e-eVRQR-P2A-EGFP (HES1425)

    Plasmid

    #242657

    Purpose

    CMV promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with neVRQR(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    pCMV-ABE8e-SpCas9-VRQR(S55R)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA, eVRQR mutations in SpCas…

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. Tad-CBE2.4

    Plasmid

    #235055

    Purpose

    Expresses Tad-CBE2.4 in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE2.4-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, SV40NLS and bpNLS

    Expression

    Mammalian

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. Tad-CBE3.6

    Plasmid

    #235058

    Purpose

    Expresses Tad-CBE3.6 in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE3.6-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, SV40NLS and bpNLS

    Expression

    Mammalian

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. Tol2_8xCTS-ECFP

    Plasmid

    #200249

    Purpose

    Zebrafish transgenics; Plasmid encodes the sgRNA2_8xCTS-ECFP reporter and could be stably integrated in the zebrafish genome using Tol2 transgenesis

    Depositor

    Tatjana Sauka-Spengler

    Article

    Pelea et al bioRxiv 2023.05.08.539738

    Insert

    8xCTS-ECFP
    

    Use

    Zebrafish expression

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. nCas9-NG_P2A-mCherry

    Plasmid

    #219934

    Purpose

    Mammalian expression of Sp-nCas9-NG with P2A-mCherry marker for use with MOBE systems

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    Sp-nCas9(D10A)-NG_P2A-mCherry
    

    Use

    CRISPR

    Tags

    SV40 bpNLS and SV40 bpNLS-P2A-mCherry

    Expression

    Mammalian

    Mutation

    nCas9(D10A)

    Promoter

    CMV

    Available Since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. JAB2497

    Plasmid

    #190378

    Purpose

    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::QacR-NLS_19St)

    Depositor

    Jennifer Brophy, Jose Dinneny

    Article

    Brophy et al Science. 2022 Aug 12;377(6607):747-751.

    Insert

    proG1090::QacR-NLS_19St
    

    Expression

    Plant

    Available Since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGC247

    Plasmid

    #19634

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Gateway(R1-R2)

    Use

    Flp/frt, destination vector

    Tags

    4xNLS

    Expression

    Worm

    Available Since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGC245

    Plasmid

    #19638

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Gateway(R4-R3)

    Use

    Flp/frt, destination vector

    Tags

    4xNLS

    Expression

    Worm

    Available Since

    March 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-ABE8.8m-VRQR-P2A-EGFP (BKS971)

    Plasmid

    #242653

    Purpose

    CMV promoter expression plasmid for human codon optimized ABE8.8m A-to-G base editor with nVRQR(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    pCMV-ABE8.8m-SpCas9-VRQR-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8.8 mutations in TadA, VRQR mutations in SpCas…

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge