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We narrowed to 25,462 results for: CHI;

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Showing: 5261 - 5280 of 25462 results

  4. pCAGGS-Twin-strep-TEV-DGKδ2-DSAMD

    Plasmid
    #223721
    Purpose
    Express human diacylglycerol kinase delta 2- SAM domain deletion mutant (N-terminal TEV protease cleavable Twin-Strep-fusion protein) in mammalian cell
    Depositor
    Chiaki Murakami
    Insert
    DGKD (DGKD Human)
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    deleted sterile alpha motif domain (deleted amino…
    Promoter
    CAG and chicken β-actin promoter
    Available Since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    223721-plasmid-map-sequence-id-457432

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  5. pCW57-CMV-MAAP-SL01V6

    Plasmid
    #218249
    Purpose
    1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.
    Depositor
    David Schaffer
    Article
    Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:
    Insert
    Membrane-Associated Accessory Protein Variant SL01V6 driven by CMV promoter
    Use
    Lentiviral
    Promoter
    CMV
    Available Since
    Oct. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    218249-plasmid-map-sequence-id-449746

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  6. pCW57-CMV-MAAP-SL01V9

    Plasmid
    #218250
    Purpose
    1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.
    Depositor
    David Schaffer
    Article
    Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:
    Insert
    Membrane-Associated Accessory Protein Variant SL01V9 driven by CMV promoter
    Use
    Lentiviral
    Promoter
    CMV
    Available Since
    Oct. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    218250-plasmid-map-sequence-id-449681

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  7. pCW57-CMV-MAAP-SL08V9

    Plasmid
    #218251
    Purpose
    1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.
    Depositor
    David Schaffer
    Article
    Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:
    Insert
    Membrane-Associated Accessory Protein Variant SL08V9 driven by CMV promoter
    Use
    Lentiviral
    Promoter
    CMV
    Available Since
    Oct. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    218251-plasmid-map-sequence-id-449683

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  9. Tol2-U6.3-sgRNA-non-targeting-control -GFP

    Plasmid
    #221844
    Purpose
    Tol2 transposon expresses control non-targeting sgRNA from chick U6.3 promoter expresses GFP reporter from GAGC promoter
    Depositor
    Stephen Terry
    Article
    O'Sullivan et al Development. 2024 Aug 1;151(15):dev20284
    Inserts
    EGFP
    non-targeting control sgRNA- GCACTGCTACGATCTACACC
    Use
    CRISPR; Tol2 transposon optimised for chick expre…
    Expression
    Mammalian
    Promoter
    GACG and U6.3 chick
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    221844-plasmid-map-sequence-id-437475

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  15. pcDNA3.1-VcnTS-E1015A

    Plasmid
    #213415
    Purpose
    Vinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).
    Depositor
    Brenton Hoffman
    Article
    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:
    Insert
    VcnTS-E1015A (VCL Chicken, Synthetic)
    Expression
    Mammalian
    Mutation
    Mutated vinculin glutamic acid 1015 to alanine (E…
    Promoter
    CMV
    Available Since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    213415-plasmid-map-sequence-id-422214

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  16. pcDNA3.1-VcnTS-E1021A

    Plasmid
    #213416
    Purpose
    Vinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).
    Depositor
    Brenton Hoffman
    Article
    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:
    Insert
    VcnTS-E1021A (VCL Chicken, Synthetic)
    Expression
    Mammalian
    Mutation
    Mutated vinculin glutamic acid 1021 to alanine (E…
    Promoter
    CMV
    Available Since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    213416-plasmid-map-sequence-id-422049

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  17. pcDNA3.1-VcnCS-E1015A

    Plasmid
    #213412
    Purpose
    Vinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).
    Depositor
    Brenton Hoffman
    Article
    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:
    Insert
    VcnCS-E1015A (VCL Chicken, Synthetic)
    Expression
    Mammalian
    Mutation
    Mutated vinculin glutamic acid 1015 to alanine (E…
    Promoter
    CMV
    Available Since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    213412-plasmid-map-sequence-id-422215

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  18. pcDNA3.1-VcnCS-E1021A

    Plasmid
    #213413
    Purpose
    Vinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).
    Depositor
    Brenton Hoffman
    Article
    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:
    Insert
    VcnCS-E1021A (VCL Chicken, Synthetic)
    Expression
    Mammalian
    Mutation
    Mutated vinculin glutamic acid 1021 to alanine (E…
    Promoter
    CMV
    Available Since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    213413-plasmid-map-sequence-id-422114

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  19. pDY279

    Plasmid
    #182959
    Purpose
    Str resistant,CoE1* ori. CRISPR/Cas9 gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations in ptsI. (for 1st round editing)
    Depositor
    Shelley Copley
    Article
    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12
    Insert
    gRNA (ttcaggcattttagcatccc), homologous repair template for ptsI
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    182959-plasmid-map-sequence-id-365361

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  20. pDY281

    Plasmid
    #182960
    Purpose
    Amp resistant, CoE1* ori. CRISPR/Cas9 induced by AHTc, gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations of ptsI. (for 2nd round editing)
    Depositor
    Shelley Copley
    Article
    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12
    Insert
    gRNA (acctggtgaagccaatattc), homologous repair template for ptsI
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    182960-plasmid-map-sequence-id-365357

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Showing: 5261 - 5280 of 25462 results