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AiP1733 - pAAV-AiE2389m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214532

Purpose

AiE2389m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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AiP1774 - pAAV-AiE2449m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214540

Purpose

AiE2449m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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AiP1333 - pAAV-AiE2115m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214484

Purpose

AiE2115m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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AiP1492 - pAAV-AiE2004m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214501

Purpose

AiE2004m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1332 - pAAV-AiE2114m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214483

Purpose

AiE2114m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-hM3D(Gq)-mCherry-shVgf-CWB

Plasmid

#223658

Purpose

Expresses hM3D(Gq) and a shRNA targetting VGF in a Cre-dependent manner

Depositor

Oscar Marin

Publication

Selten et al Nature. 2025 Apr 30. doi: 10.1038/s41586

Insert

hM3D(Gq)-mCherry and shVGF

Use

AAV and RNAi

Tags

mCherry

Expression

Mammalian

Promoter

hSyn

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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MTOR-F2108L_CD19-CAR-28z-2A-EGFP_AAV6_Donor

Plasmid

#211904

Purpose

Vector for AAV6 donor production. Targeted CD19-CAR-28z-2A-EGFP transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.

Depositor

Yannick Doyon

Publication

Levesque et al bioRxiv 2023.09.14.557485

Insert

CD19-CAR-28z-2A-EGFP

Use

AAV

Expression

Mammalian

Promoter

hPGK1

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVf-EnhCB-lacZnls miR-122 3x

Plasmid

#35644

Depositor

Phillip Zamore

Publication

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

3 miR-122 target sites

Use

AAV

Promoter

CB

Available since

April 25, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-UbC-CytoTape-HA

Plasmid

#239423

Purpose

CytoTape structural monomer for CytoTape fiber formation and elongation for cell culture applications

Depositor

Changyang Linghu

Publication

Zheng et al Nature. 2026 Jan 26. doi: 10.1038/s41586

Insert

CytoTape-HA

Use

AAV

Tags

HA-dMBP

Expression

Mammalian

Promoter

UbC promoter

Available since

Nov. 12, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-N-RAM-CytoTape-V5

Plasmid

#239425

Purpose

CytoTape signal monomer for recording Npas4 protein activity

Depositor

Changyang Linghu

Publication

Zheng et al Nature. 2026 Jan 26. doi: 10.1038/s41586

Insert

CytoTape-V5

Use

AAV

Tags

V5-dMBP

Expression

Mammalian

Promoter

N-RAM promoter

Available since

Nov. 12, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-HSE-CytoTape-V5

Plasmid

#239426

Purpose

CytoTape signal monomer for recording HSE promoter transcriptional activity

Depositor

Changyang Linghu

Publication

Zheng et al Nature. 2026 Jan 26. doi: 10.1038/s41586

Insert

CytoTape-V5 (HSPA1A Synthetic)

Use

AAV

Tags

V5-dMBP

Expression

Mammalian

Promoter

HSPA1A promoter

Available since

Oct. 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV.GFAP.(ER).iGlucoSnFR2.HaloTag

Plasmid

#244090

Purpose

ER targeted expression of green glucose sensor with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Publication

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.HaloTag

Use

AAV

Tags

IgG kappa leader and mIRFP670nano3

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_AR-RE_MLPmin_BC1013-luc2

Plasmid

#227107

Purpose

Androgen receptor response element for luciferase and barcode assays; barcode BC1013

Depositor

Michael Wehr

Publication

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

8x clustered AR response element linked to MLP minimal promoter driving barcode 1013 and a luciferase reporter gene

Use

AAV

Expression

Mammalian

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.RMacL5ET-01.EGFP.BCA7.WPRE

Plasmid

#225689

Purpose

L5 extratelencephalic projection neuron Enhancer to drive transgene expression

Depositor

William Stauffer

Publication

He et al Neuron. 2025 May 21;113(10):1548-1561.e8

Insert

RMacL5ET-01.EGFP.BCA7

Expression

Mammalian

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MCHpr-GCaMP6f

Plasmid

#221039

Purpose

Expresses GCaMP6f under the rat pro-melanin-concentrating hormone promoter

Depositor

Yanhua Huang

Publication

Guo et al Biol Psychiatry. 2022 Dec 1;92(11):880-8

Insert

GCaMP6f

Use

AAV; Rat targeting

Expression

Mammalian

Mutation

A317E

Promoter

Rat MCH

Available since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miRFP2

Plasmid

#178968

Purpose

Expresses miRFP2 in mammalian cells

Depositor

Kiryl Piatkevich

Publication

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

miRFP2

Use

AAV

Tags

no

Expression

Mammalian

Mutation

NO

Promoter

CAG

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAAVsc.U6-sgGTTG.CMV/CB-Gluc

Plasmid

#121512

Purpose

Expresses sgRNA targeting the intron in the MADM alleles (GT and TG).

Depositor

Guangping Gao

Publication

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgGTTG

Use

AAV

Expression

Mammalian

Promoter

U6

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-DIO-SEP-GluA1R

Plasmid

#227236

Purpose

Large cargo capacity AAV that expresses SEP tagged AMPA receptor subunit GluA1 Q582R mutant in neurons expressing Cre recombinase

Depositor

Richard Huganir

Publication

Hong et al Nature. 2024 Nov;635(8038):398-405. doi:

Insert

SEP-GluA1 Q582R (Gria1 Rat)

Use

AAV

Tags

superecliptic pHluorin

Expression

Mammalian

Mutation

Q582R

Promoter

hSyn1

Available since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pOTTC1079 - pAAV TH gRNA A EF1a EGFP

Plasmid

#113159

Purpose

An AAV vector that expresses guide RNA targeting rat TH and expresses EGFP reporter

Depositor

Brandon Harvey

Publication

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

gRNA for rat TH

Use

AAV and CRISPR

Expression

Mammalian

Promoter

mU6

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-JEDI3hyp-WPRE

Plasmid

#246637

Purpose

Genetically encoded voltage indicator (GEVI) JEDI3hyp in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKIIa

Depositor

Francois St-Pierre

Publication

Land et al Nat Methods. 2026 Apr 15. doi: 10.1038/s

Insert

JEDI3hyp

Use

AAV

Expression

Mammalian

Promoter

CaMKIIa

Available since

April 23, 2026

Availability

Academic Institutions and Nonprofits only

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