We narrowed to 14,047 results for: crispr grnas

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LCV2_mCherry_Luciferase_sgRNA

Plasmid

#155109

Purpose

lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting Luciferase

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

Luciferase_sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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LCV2_Luciferase_sgRNA

Plasmid

#155094

Purpose

lentiviral plasmid expressing Cas9 and gRNA targeting Luciferase

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

Luciferase_sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pC131: pAAV.CMV-CasRx-VEGFA sgRNA array

Plasmid

#203444

Purpose

Plasmid expressing active RfxCas13d with an array of three VEGFA mRNA targeting gRNAs

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA array
RfxCas13d

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pM156: pAAV.CMV-Cas13e (GenScript CO)-VEGFA sgRNA

Plasmid

#203452

Purpose

Plasmid expressing active and codon optimised Cas13e with VEGFA gRNA

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA
Cas13e

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGW02 AAV-TBG-Cre-sgP53-sgRNA

Plasmid

#192162

Purpose

AAV-TBG-Cre-sgP53-sgRNA

Depositor

Sidi Chen

Article

Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

Type

Empty backbone

Use

AAV

Mutation

NA

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-1-3'

Plasmid

#117324

Purpose

CRISPR-Cas9 for miR-124-1, 3'

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

gRNA miR-124-1-3' (MIR124-1 Synthetic)

Tags

3xFLAG

Expression

Mammalian

Promoter

U6

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-3-5'

Plasmid

#117323

Purpose

CRISPR-Cas9 for miR-124-3, 5'

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

gRNA miR-124-3-5' (MIR124-3 Synthetic)

Tags

3xFLAG

Expression

Mammalian

Promoter

U6

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W

Plasmid

#67979

Purpose

Cas9 activity reporter (control) with BFP and GFP.

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKBFP2AGFP cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Icam 2 SaCas9 YAP sgRNA1

Plasmid

#99734

Purpose

Expresses SaCas9 and a gRNA targeting mouse YAP

Depositor

Yu Huang

Article

Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

Insert

SaCas9 gRNA targeting Yap1 (Yap1 Mouse)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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Icam 2 SaCas9 YAP sgRNA2

Plasmid

#99735

Purpose

Expresses SaCas9 and a gRNA targeting mouse YAP

Depositor

Yu Huang

Article

Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

Insert

SaCas9 gRNA targeting Yap1 (Yap1 Mouse)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pSpCas9nucl-T2A-EGFP - BAXgRNA1- BAXgRNA2

Plasmid

#167296

Purpose

Plasmid encoding for 2 gRNAs targeting the human BAX gene and a CMV driven nuclease Cas9 followed by self-cleaving EGFP

Depositor

Agnel Sfeir

Article

Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

Insert

BAX (BAX Human)

Expression

Mammalian

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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gRNA_Cloning Vector BbsI

Plasmid

#128433

Purpose

Embty backbone vector for expression of gRNA for spCas9 genome editing or epigenome editing

Depositor

Izuho Hatada

Type

Empty backbone

Expression

Mammalian

Promoter

U6 promoter

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDY1513 AAVS1 sgRNA

Plasmid

#234832

Purpose

sgRNA for AAVS1 STITCHR insertion

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

Insert

AAVS1 sgRNA

Use

CRISPR

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pU6 SgRNA RBM20

Plasmid

#162735

Purpose

Mammalian expression

Depositor

Amit Choudhary

Article

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Insert

Spacer sequence for RBM20

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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L2_lacZgRNA-Cas9-CsA

Plasmid

#136138

Purpose

Plasmid to accept gRNA target with SapI, lacZ blue-white screening. Contains Cas9 and HygR.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-35Sx2:HygR p5-MpU6:lacZgRNA p5-MpEF1a:Cas9-NLS

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-RHOA_sgRNA

Plasmid

#183878

Purpose

pX459V2.0-HypaCas9 plasmid with RHOA sgRNA for N-terminal tagging of RhoA in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

RHOA sgRNA spacer (RHOA Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-ABE-NT-sgRNA

Plasmid

#112734

Purpose

AAV inverted terminal repeat based vector plasmid encoding E. coli TadA, the N-terminal half of nCas9 and sgRNA

Depositor

Jin-Soo Kim

Article

Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.

Insert

ABE-NT-sgRNA

Use

AAV

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-ACTB_sgRNA

Plasmid

#183885

Purpose

pX459V2.0-HypaCas9 plasmid with ACTB sgRNA for N-terminal tagging of beta-actin in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

ACTB sgRNA spacer (ACTB Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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px330-UFM1 sgRNA2

Plasmid

#134634

Purpose

contains sgRNA targeting human UFM1 for gene knockout

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFM1 sgRNA2 (UFM1 Human)

Expression

Mammalian

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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px330-UFM1 sgRNA1

Plasmid

#134633

Purpose

contains sgRNA targeting human UFM1 for gene knockout

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFM1 sgRNA1 (UFM1 Human)

Expression

Mammalian

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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