We narrowed to 7,267 results for: trac

1. pMflET-o4

   Plasmid

   #101314

   Purpose

   Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and ereB resistance genes, origin of transfer of RP4 plasmid.

   Depositor

   Sébastien Rodrigue

   Article

   Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

   Inserts

   oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
   ereB resistance gene
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDY118A

   Plasmid

   #182958

   Purpose

   pSC101 ori, chl34 resistant. Cas9 induced at 0.4 μg/ml anhydrotetracycline, recombineering proteins induced by a 15 min heat-shock at 42°C, I-Scel (induced by 0.1 mM IPTG) is to cleave the pDonor2.

   Depositor

   Shelley Copley

   Article

   Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12

   Inserts

   Cas9
   Gam, Beta, Exo
   I-scel
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTripz Casp1-IRES-mCherry

   Plasmid

   #187294

   Purpose

   This is Caspase1 from mouse (NM_009807.2) with its CARD domain replaced by the FKBP-V domain (dimerising domain).

   Depositor

   Alpha Yap

   Article

   Bonfim-Melo et al Cell Rep. 2022 Feb 1;38(5):110316. doi:

   Insert

   Caspase1 (Casp1 Mouse)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   CMV

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV iGluu

   Plasmid

   #105832

   Purpose

   Fast indicator for extracellular glutamate imaging

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGluSnFR S72T variant
   

   Use

   Tags

   Myc and PDGFR

   Expression

   Mammalian

   Mutation

   Changed Ser 72 to Thr

   Promoter

   CMV

   Available since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCEP4-myc-H2-Dd

   Plasmid

   #135518

   Purpose

   Mammalian expression of myc-tagged H2-Dd

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   H2-Dd (H2-D1 Mouse)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   C121S

   Promoter

   CMV

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMRE-Tn5-162

   Plasmid

   #118543

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   sGFP2
   

   Use

   Minitn5 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMRE-Tn7-161

   Plasmid

   #118573

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mTurquoise2
   

   Use

   Minitn7 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRE-Tn7-166

   Plasmid

   #118578

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mCardinal
   

   Use

   Minitn7 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPICZ-pre-pro-alphaf-E2-Crimson

   Plasmid

   #117658

   Purpose

   Test intracellular localisation of E2-Crimson and study the secretion efficiency

   Depositor

   Pau Ferrer, Benjamin Glick

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   pre-pro-alphaf-E2-Crimson
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   This plasmid encodes the fluorescent protein E2-C…

   Promoter

   pAOX1

   Available since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 V5 His-TOPO-GluCl optbeta-m-YFP Y182F RSR_AAA (Opt β-Gluclv2.0)

    Plasmid

    #47542

    Purpose

    engineered receptor improves heteromeric receptor expression and sensitivity to IVM.

    Depositor

    Henry Lester

    Article

    Frazier et al J Biol Chem. 2013 May 29.

    Insert

    Glutamate-gated chloride channels
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    retention motif in ER (starting at R340) changed …

    Promoter

    CMV

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSCT-GAL93-SFPQ

    Plasmid

    #46320

    Depositor

    Steven Brown

    Article

    Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

    Insert

    SFPQ (SFPQ Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pMSCV-IRES-GFP-CD4-TLR4 cyto

    Plasmid

    #133064

    Purpose

    Retroviral expression of mouse CD4 extracellular domain fused with the cytosolic tail of TLR4

    Depositor

    Jonathan Kagan

    Article

    Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

    Insert

    CD4-TLR4cyto (Tlr4 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-syn-SnFR-gamma8-minWPRE

    Plasmid

    #165498

    Purpose

    AAV for glutamate reporter fused to TARP gamma-8

    Depositor

    Andrew Plested

    Article

    Hao et al eLife. 2023 Jan 9;12:e84029. doi: 10.755

    Insert

    iGluSnFR extracellular domain fused to TARP gamma-8 via NETO2 TM domain (Cacng8 Synthetic, Rat)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSynapsin

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAH-CTX1-rhadCas9-native

    Plasmid

    #129392

    Purpose

    Derived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Aspartate 10 to Alanine, Histidine 840 to Alanine

    Promoter

    PrhaBAD

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. sq5

    Plasmid

    #24499

    Depositor

    Bruce Conklin

    Article

    Conklin et al Mol Pharmacol. 1996 Oct . 50(4):885-90.

    Insert

    Gs alpha (Gnas Rat)
    

    Use

    Tags

    HA (see comments)

    Expression

    Mammalian

    Mutation

    This is Gs alpha with the C-terminal amino acids …

    Promoter

    Available since

    Aug. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

16. CMV-10xPP7 oligo-library based

    Plasmid

    #158199

    Purpose

    Cassette of 10 different binding sites with high affinity to PP7 phage coat protein, as extracted from the oligo pool experiment.

    Depositor

    Roee Amit

    Article

    Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:

    Insert

    10 binding sites of PP7 from the oligo library
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. cfmTurquoise-N

    Plasmid

    #139284

    Purpose

    Cys-free mTurquoise (improved ECFP) for extracellular FRET analysis. This variant contains the same set of mutations (C48S and C70M ) as cfSGFP2. Molecular brightness is similar to mTurquoise.

    Depositor

    Ikuo Wada

    Article

    Omae et al Transfusion. 2019 Jul;59(7):2429-2435. d

    Insert

    cfmTurquoise
    

    Use

    Tags

    Multi-cloning sites

    Expression

    Mammalian

    Mutation

    Cys48Ser and Cys70/Met

    Promoter

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDM1383

    Plasmid

    #207423

    Purpose

    Extrachromosomal expression HSPC300-GFP and mCherry-Hs Raf1 (1–134 RBD)

    Depositor

    Rob Kay

    Article

    Veltman et al Elife. 2016 Dec 13;5:e20085. doi: 10.755

    Inserts

    HSPC300
    Raf1 (RAF1 Human)
    

    Use

    Unspecified

    Tags

    GFP and mCherry

    Expression

    Mutation

    aa 1–134 (RBD) only

    Promoter

    Available since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCXLE-hS + eGFP

    Plasmid

    #74945

    Purpose

    Dosage control and tracing of reprogramming episomes

    Depositor

    Ann Zovein

    Article

    Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

    Insert

    Sox2 (SOX2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

20. pET28a(DAP10)

    Plasmid

    #177904

    Purpose

    Bacterial expression of his10-tagged human DAP10 Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    DAP10 (aa 70-92 only) (HCST Human)
    

    Use

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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