We narrowed to 1,231 results for: POLI

1. pLV-EF1A-HUWE1-1503-1780_EGFP-Hygro

   Plasmid

   #238096

   Purpose

   Expression of structurally predicted WWE domain of HUWE1 with EGFP on C terminal

   Depositor

   Robert Sobol

   Article

   Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 1-166 only

   Promoter

   EF1alpha

   Available Since

   Aug. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-Hygro-EF1A-Af1521(K35E/Y145R)-myc

   Plasmid

   #196237

   Purpose

   Af1521(K35E/Y145R) macrodomain with a myc tag fused to the C terminus & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

   Insert

   Af1521(K35E/Y145R) encoding a C-terminal myc tag
   

   Use

   Lentiviral

   Tags

   myc tag

   Expression

   Mammalian

   Mutation

   amino acid 35 lysine is replaced with glutamic ac…

   Promoter

   EF1A

   Available Since

   Aug. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-4T-3-GST-Tev-Af1521-myc

   Plasmid

   #196239

   Purpose

   N-terminal GST fusion of Af1521(WT) with a TEV protease site located between the GST tag and Af1521 and a myc tag on the C terminus

   Depositor

   Robert Sobol

   Article

   Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

   Insert

   N-terminal GST fusion of Af1521(WT) with a TEV protease site between GST and Af1521 encoding a C-terminal myc tag
   

   Tags

   GST tag and myc tag

   Expression

   Bacterial

   Available Since

   Aug. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. yTREX-ApR

   Plasmid

   #177307

   Purpose

   genomic integration of genes (inserted in I-SceI-site) via transposon Tn5

   Depositor

   Karl-Erich Jaeger

   Article

   Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

   Type

   Empty backbone

   Use

   Synthetic Biology; Yeast expression, tn5 genomic …

   Expression

   Bacterial

   Available Since

   Aug. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. ABEmax-Puro V2

   Plasmid

   #226955

   Purpose

   ABEmax plasmid enabling A:T to G:C base editing using a single plasmid.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. DG SaCas9 GFP V3

   Plasmid

   #226961

   Purpose

   CBh-SaCas9-2A-GFP, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. DG SaCas9 puro V3

   Plasmid

   #226960

   Purpose

   CBh-SaCas9-2A-Puro, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. rUBR5_2383-2442_pGEX6P1

   Plasmid

   #209791

   Purpose

   Bacterial expression of UBR5 Mlle domain

   Depositor

   Kalle Gehring

   Article

   Lim et al J Biol Chem. 2006 May 19;281(20):14376-8

   Insert

   Mlle domain of UBR5 (Ubr5 Rat)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 2383-2442 only

   Promoter

   tac

   Available Since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. LARP1_323-509

   Plasmid

   #209800

   Purpose

   Bacterial expression of human LARP1 La-module

   Depositor

   Kalle Gehring

   Article

   Kozlov et al Nucleic Acids Res. 2022 Sep 9;50(16):953

   Insert

   LARP1 La-module (LARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   aa 323-509 only

   Promoter

   T7

   Available Since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. LARP1_323-410

    Plasmid

    #209801

    Purpose

    Bacterial expression of human LARP1 LaM domain

    Depositor

    Kalle Gehring

    Article

    Kozlov et al Nucleic Acids Res. 2022 Sep 9;50(16):953

    Insert

    LARP1 LaM (LARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 323-410 only

    Promoter

    T7

    Available Since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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