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Plasmid#153284PurposeThe attB plasmid harboring the Hsp70Bb-Cas9-T2A-eGFP-p10 and a mini-white marker.DepositorInsertpHsp70Bb-SpCas9-T2A-eGFP-p10 (cas9 Synthetic)
UseCRISPRTagseGFPExpressionInsectMutationPromoterDmel Hsp70BbAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-01*-(ΔLF)
Plasmid#80045PurposeExpression of Vpr binding mutant EPN-01*DepositorInsertEPN-01*(L33R)
UseTagsMycExpressionMammalianMutationVpr binding site LF45 in p6 mutatedPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-01(ΔL1+L2)
Plasmid#80051PurposeExpression of EPN-01 mutated in both L domainsDepositorInsertEPN-01(deltaPTAP/deltaYP)
UseTagsMycExpressionMammalianMutationPTAP10AAAA, YP37AAPromoterCMVAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-01*(ΔI)
Plasmid#80041PurposeExpression of EPN-01 assembly mutant L33RDepositorInsertEPN-01*(L33R)
UseTagsMycExpressionMammalianMutationchanged Leu33 in I3-01 to AlaPromoterCMVAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJL1-sfGFP-DQNAT
Plasmid#198210PurposepJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing an optimal DQNAT sequon followed by a 6x-His tagDepositorInsertsfGFP_DQNAT
UseTags6x His Tag and DQNAT glycosylation tagExpressionBacterialMutationPromoterT7Available sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-VSP4-E228Q
Plasmid#80351PurposeExpression of GFP-tagged, dominant negative VPS4 mutantDepositorInsertVPS4-E228Q (VPS4A Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
OA-1067B
Plasmid#200252PurposepBac-U6-gRNA(doublesex)-3xp3-tdTomatoDepositorInsert4 gRNAs targeting Doublesex
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti.PGK.blast-Renilla_Luciferase
Plasmid#74444PurposeControl lentiviral vector expressing Renilla luciferaseDepositorInsertRenilla Luciferase
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDMS151A_Tet-On-3G
Plasmid#191577PurposeClonTech-Takara's doxycycline-inducible, rtTA transactivator (Tet-On-3G) cloned into a 2nd generation lentiviral transfer plasmid (pGIPZ).DepositorInsertTet-On(R) 3G
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVSL10
Plasmid#201182PurposeBst polymerase large fragment (BF) F710Y/D598A, His6-tagged, HRV 3C-cleavableDepositorInsertBst polymerase large fragment (BF) F710Y/D598A
UseTagsHRV 3C protease cleavage site and His6ExpressionBacterialMutationF710Y D598APromoterT5-lacOAvailable sinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only