We narrowed to 3,227 results for: multiplex

1. pVITRO1-TagRFP-NLS

   Plasmid

   #184446

   Purpose

   Nucleus-targeted TagRFP expression in mammalian cells

   Depositor

   Chuan-Hsiang Huang

   Article

   Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

   Insert

   TagRFP-3xNLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Rat EF1α

   Available since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVITRO1-mCardinal-NES

   Plasmid

   #184453

   Purpose

   Cytosolic mCardinal expression in mammalian cells

   Depositor

   Chuan-Hsiang Huang

   Article

   Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

   Insert

   mCardinal-NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Rat EF1α

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330S-4

   Plasmid

   #58780

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ141C

   Plasmid

   #69292

   Purpose

   Gateway entry vector; single gRNA under OsU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO_NES_HaloTag9

   Plasmid

   #175537

   Purpose

   Strict cytosolic expression of HaloTag9 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   NES-HaloTag9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSALECT-EcoBam

   Plasmid

   #59705

   Purpose

   Folding reporter with ampicillin resistance as a readout

   Depositor

   Christopher Anderson

   Article

   Hsiau et al PLoS One. 2015 Mar 19;10(3):e0119927. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA5/FRT/TO_[Cox8a]x2_HaloTag7

   Plasmid

   #175529

   Purpose

   Expression of HaloTag7 at the inner membrane of mitochondria in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Cox8a-HaloTag7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. NM1-5S-tRNA-SgH

   Plasmid

   #128178

   Purpose

   Expresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoter

   Depositor

   Huimin Zhao

   Article

   Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

   Insert

   gRNA cloning cassette
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   5S rRNA-tRNA(Arg)

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. 2xMyc-tdMCP-SNAP (tdMCP standard)

   Plasmid

   #226166

   Purpose

   For transient expression of the tdMCP-protein standard. Used in LABEL-seq abundance assays.

   Depositor

   Dustin Maly

   Article

   Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

   Insert

   2xMyc-tdMCP-SNAPtag
   

   Use

   Lentiviral

   Tags

   2xMyc-tdMCP and EGFP-IRES-BlastR

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pScI_dCas9-CDA

    Plasmid

    #108549

    Purpose

    Bacterial Target-AID vector

    Depositor

    Akihiko Kondo

    Article

    Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

    Insert

    dCas9-PmCDA1
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    D10A and H840A for SpCas9

    Promoter

    lambda OR

    Available since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEX128·gRNA

    Plasmid

    #187600

    Purpose

    Template for amplification of gRNA with Cas6 recognition site

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

    Insert

    gRNA template
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    no promoter

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330A-1x3

    Plasmid

    #58767

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHAGE-EFS-PCP-3XNeon-nls

    Plasmid

    #75386

    Purpose

    PCP-3XNeon-nls

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    PCP
    

    Use

    Lentiviral

    Tags

    3XmNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. NM9-SpCas9-NLS3

    Plasmid

    #128177

    Purpose

    Expresses R. toruloides codon-optimized SpCas9 under PGK1 promoter

    Depositor

    Huimin Zhao

    Article

    Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

    Insert

    SpCas9-NLS3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pPGK1

    Available since

    April 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb

    Plasmid

    #175534

    Purpose

    Expression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    IgKchL_HA-HaloTag7-myc_PDGFRtmb
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX330S-5

    Plasmid

    #58781

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLH-sgRNA1-2XboxB

    Plasmid

    #75391

    Purpose

    sgRNA1-2XboxB

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-2XboxB
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCR8-attR4-pegRNA-attR3

    Plasmid

    #213056

    Purpose

    Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attR4 and attR3

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Insert

    sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    35S-CmYCLV-AtU6

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCR8-attL5-pegRNA-attL4

    Plasmid

    #213055

    Purpose

    Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attL4

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Insert

    sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    35S-CmYCLV-AtU6

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCR8-attL1-pegRNA-attR5

    Plasmid

    #213053

    Purpose

    Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR5

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Insert

    sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    35S-CmYCLV-AtU6

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge