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pEB0_14_QS

Plasmid

#236217

Purpose

Plasmid encoding QS as a Type 3 part to be used in the Dueber Yeast MoClo Toolkit.

Depositor

Yvonne Nygård

Article

Blick et al Synth Biol (Oxf). 2025 Dec 4;10(1):ysaf0

Insert

QS

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 18, 2026

Availability

Academic Institutions and Nonprofits only

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pDBB62

Plasmid

#248965

Purpose

Optogenetic expression of NShh driven by five GAL4/GAVPO binding sites in front of a synthetic YB-TATA minimal promoter in a PiggyBac integration plasmid.

Depositor

James Briscoe

Article

Benzinger et al Dev Cell. 2025 Aug 19:S1534-5807(25)0049

Insert

NShh-IRES2-H2B-mScarletI

Expression

Mammalian

Promoter

UASybTATA

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Sec7-4V5-APEX2-URA

Plasmid

#207060

Purpose

Expressing Sec7-4V5-APEX2. Insufficient signal for EM.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Sec7

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pSEC7

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Snf7-4V5-APEX2-URA

Plasmid

#207058

Purpose

Expressing Snf7-4V5-APEX2. Insufficient signal for EM.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Snf7

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pSNF7

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-THP-(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

Plasmid

#242904

Purpose

Expresses cytosolic low affinity ratiometric ATP sensor in Dopaminergic Neurons

Depositor

Timothy Ryan

Article

Pulido et al Proc Natl Acad Sci U S A. 2025 Dec 16;12

Insert

(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

Use

Lentiviral

Expression

Mammalian

Promoter

rat Tyrosine hydroxylase

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pMITO-mTFP1

Plasmid

#244030

Purpose

anchoring mTFP1 to the mitochondrial membrane

Depositor

Markus Affolter

Article

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

CISD1-mTFP1

Tags

sunTagV4

Expression

Mammalian

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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CAG-rtTA-2A-tagBFP-bGH_pKG3264

Plasmid

#246372

Purpose

Expression plasmid for rtTA-2A-tagBFP driven from the CAG promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

rtTA

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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EFS-rtTA-2A-mRuby2-bGH_pKG2064

Plasmid

#246373

Purpose

Expression plasmid for rtTA-2A-mRuby2 driven from the EFS promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

rtTA

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-iRFP670-SV40_pKG726

Plasmid

#246374

Purpose

Expression plasmid for iRFP670 driven from the EF1a promoter. Co-transfection marker for transfection experiments.

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

iRFP670

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-rtTA-BG-CMV-PuroR-BGH_pKG1701

Plasmid

#246376

Purpose

Expression plasmid for rtTA and PuroR

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

rtTA

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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RcP9_AraC_S170_LOV_P190_Sil_90bp

Plasmid

#242158

Purpose

Recombineering Plasmid 9. Positive control to test for successful editing using AraC S170 LOV (silent mutation of P190, 90bp retron)

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

Gene III

Expression

Bacterial

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-DARPin 1238_G01-YPet

Plasmid

#244039

Purpose

anti mTFP1DARPin_G01 fused to YPet

Depositor

Markus Affolter

Article

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_G01-YPet

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-DARPin 1238_E11-YPet

Plasmid

#244038

Purpose

anti mTFP1DARPin_E11 fused to YPet

Depositor

Markus Affolter

Article

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_E11-YPet

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-DARPin 1238_G01-mCherry

Plasmid

#244029

Purpose

anti mTFP1DARPin_G01 fused to mCherry

Depositor

Markus Affolter

Article

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_G01-mCherry

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-DARPin 1238_E11-mCherry

Plasmid

#244028

Purpose

anti mTFP1DARPin_E11 fused to mCherry

Depositor

Markus Affolter

Article

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_E11-mCherry

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mTFP1-Rab5c

Plasmid

#244031

Purpose

fusion of Rab5c to mTFP1

Depositor

Markus Affolter

Article

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

mTFP1-Rab5c

Expression

Mammalian

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pRRL_CMVp-EGFP

Plasmid

#244970

Purpose

Express EGFP under CMV promoter. Xho I-CMVp-AgeI-EGFP.

Depositor

Xinnan Wang

Article

Kwak et al Biochim Biophys Acta Gene Regul Mech. 20

Insert

CMVp-EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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AGG1983 UbL40-mCherry

Plasmid

#242440

Purpose

Ae aegypti UbL40 promoter expressing mCherry

Depositor

Luke Alphey

Article

Nevard et al Commun Biol. 2025 Aug 4;8(1):1154. doi:

Insert

mCherry

Expression

Insect

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EPB41L4A-AS1

Plasmid

#242748

Purpose

Expresses EPB41L4A-AS1 in mammalian cells

Depositor

Igor Ulitsky

Article

Monziani et al bioRxiv 2025.04.08.647803

Insert

EPB41L4A-AS1 (EPB41L4A-AS1 Human)

Expression

Mammalian

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Puro-CMV-EPB41L4A-AS1 unspliced-WT

Plasmid

#242749

Purpose

Expresses unspliced EPB41L4A-AS1 in mammalian cells

Depositor

Igor Ulitsky

Article

Monziani et al bioRxiv 2025.04.08.647803

Insert

EPB41L4A-AS1 (EPB41L4A-AS1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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VEE-moxBFP-IRES-PuroR

Plasmid

#242413

Purpose

Spectral unmixing: moxBFP only

Depositor

Ewan St. John Smith

Article

Lim et al bioRxiv 2024.09.24.614636

Inserts

moxBFP
IRES-PuroR

Use

Self-amplifying rna

Expression

Mammalian

Available Since

Sept. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-miRFP703-dSal-VP16minADx3-hCRY2-NLS

Plasmid

#241843

Purpose

Expresses an actuator of Opto-p53 in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Tsuruoka et al Cell Struct Funct. 2025 Jun 12. doi: 10.

Insert

VP16

Tags

CRY2 and miRFP703

Expression

Mammalian

Mutation

VP16 minimal activation domain x 3

Promoter

CAGGS promoter

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGY240

Plasmid

#239811

Purpose

Express H3-EGFP and H2B-mRFP1 simultaneously in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Inserts

H3
H2B

Use

Fungal expression

Tags

EGFP and mRFP1

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGY251

Plasmid

#239813

Purpose

Express H2B-NLS-EGFP in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

NLS of H2B

Use

Fungal expression

Tags

EGFP

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGY203

Plasmid

#239802

Purpose

Express GUS in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

GUS

Use

Fungal expression

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGY209

Plasmid

#239804

Purpose

Express mRFP1 in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

mRFP1

Use

Fungal expression

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGY219

Plasmid

#239806

Purpose

Express H2B-EGFP in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

H2B

Use

Fungal expression

Tags

EGFP

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGY220

Plasmid

#239807

Purpose

Express H3-EGFP in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

H3

Use

Fungal expression

Tags

EGFP

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGY224

Plasmid

#239808

Purpose

Express H2B-mRFP1 in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

H2B

Use

Fungal expression

Tags

mRFP1

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGY225

Plasmid

#239809

Purpose

Express H3-mRFP1 in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

H3

Use

Fungal expression

Tags

mRFP1

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGY232

Plasmid

#239810

Purpose

Express EGFP-P2A-mRFP1 in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Inserts

EGFP
mRFP1

Use

Fungal expression

Tags

2A

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pWormgate2-myo-2p-gfp-halo

Plasmid

#227169

Purpose

For organ-specific expression of GFP-Halo in the pharyngeal muscle of C. elegans.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

GFP

Tags

Halotag

Expression

Worm

Promoter

myo-2

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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BtA2

Plasmid

#238982

Purpose

In vitro transcription of bovine arrestin-2 long isoform

Depositor

Jeffrey Benovic, Vsevolod Gurevich

Article

Sterne-Marr et al J Biol Chem. 1993 Jul 25;268(21):15640-8

Insert

arrestin-2 long (ARRB2 Bovine)

Use

In vitro transcription

Mutation

none

Promoter

SP6

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRSET-cfp29

Plasmid

#233651

Purpose

Mycobacterium tuberculosis Encapsulin CFP29 expression plasmid in E. coli

Depositor

Ye Gao

Article

Berger et al Commun Biol. 2025 Feb 15;8(1):245. doi:

Insert

Encapsulin

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRO286

Plasmid

#233812

Purpose

MoClo-YTK; type2A; Auxin systems; amtRO7

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

amtRO7

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CT751

Plasmid

#233657

Purpose

Dox-inducible VP64-MyoD

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

VP64-MyoD

Expression

Mammalian

Mutation

None

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CT754

Plasmid

#233659

Purpose

FLEx switch with tTA

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

tTA FLEx switch

Expression

Mammalian

Mutation

None

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ dgat1b-FLAG

Plasmid

#232144

Purpose

Expresses zebrafish dgat1b with a C-terminal FLAG tag under control of the CMV promoter

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

dgat1b

Use

Zebrafish expression

Tags

FLAG

Promoter

CMV

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-ABCB10-eYFP

Plasmid

#206038

Purpose

Express YFP in mitochondria

Depositor

Lufang Zhou

Article

Ernst et al Biophys J. 2019 Aug 20;117(4):631-645. d

Insert

EYFP

Tags

ABCB10 mitochondrial leading sequence

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGlab592

Plasmid

#236554

Purpose

240 basepair upstream sequence of KSHV ORF68 driving HaloTag expression

Depositor

Britt Glaunsinger

Article

Morgens et al Mol Syst Biol. 2025 Jan;21(1):30-58. doi

Insert

KSHV ORF68 upstream 240bp

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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