Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-2f)

Plasmid

#241063

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-2f) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-2f) (S SARS-CoV-2)

Expression

Mammalian

Mutation

S494R,K147N,T19I,L24del,P25del,P26del,A27S,G142D,…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-2c)

Plasmid

#241060

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-2c) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-2c) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,G142D,V213G,G339D,…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-2d)

Plasmid

#241061

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-2d) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-2d) (S SARS-CoV-2)

Expression

Mammalian

Mutation

F490R,S494R,T19I,L24del,P25del,P26del,A27S,G142D,…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-2a)

Plasmid

#241058

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-2a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-2a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,G142D,V213G,G339D,…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-2h)

Plasmid

#241059

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-2h) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-2h) (S SARS-CoV-2)

Expression

Mammalian

Mutation

R346T,S494R,T19I,L24del,P25del,P26del,A27S,G142D,…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgSHMT2

Plasmid

#217435

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human SHMT2

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA targeting SHMT2 (SHMT2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 F456L (CTT)

Plasmid

#212542

Purpose

Encodes SARS-CoV-2 Spike F456L for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike F456L (CTT) (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

F456L

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-EGFP-GAPDH-CC

Plasmid

#225476

Purpose

Express mEGFP-GAPDH-CC fusion

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

GAPDH fused with CC2 domain of ROCK2 (GAPDH Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-EGFP-GAPDH-Tudor

Plasmid

#225479

Purpose

Express mEGFP-GAPDH-Tudor fusion

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

GAPDH fused with Tudor domain of TDRD3 (GAPDH Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-EGFP-GAPDH-RGG

Plasmid

#225477

Purpose

Express mEGFP-GAPDH-RGG fusion

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

GAPDH fused with RGG domain of TOP3B (GAPDH Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 G75V (GTA) T76I (ATT)

Plasmid

#212443

Purpose

Encodes SARS-CoV-2 spike G75V, T76I for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike G75V, T76I (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

G75V, T76I

Promoter

CAG

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 HV.1

Plasmid

#212573

Purpose

Encodes SARS-CoV-2 spike for HV.1 pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike HV.1 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 del E484

Plasmid

#212564

Purpose

Encodes SARS-CoV-2 spike E484 deletion for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike E484 deletion (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

E484 deletion

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 BA.4

Plasmid

#212519

Purpose

Encodes SARS-CoV-2 variant BA.4 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike BA.4 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

T19I ,deltaL24P25P26 ,A27S ,delH69-V70 ,G142D ,V2…

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 D405N (AAC) R408S (AGC)

Plasmid

#212496

Purpose

Encodes SARS-CoV-2 spike D405N, R408S for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike D405N, R408S (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

D405N, R408S

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 N679K (AAA) P681H (CAC)

Plasmid

#212480

Purpose

Encodes SARS-CoV-2 spike N679K, P681H for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike N679K, P681H (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

N679K, P681H

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 N440K (AAG) G446S (AGC)

Plasmid

#212469

Purpose

Encodes SARS-CoV-2 spike N440K, G446S for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike N440K, G446S (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

N440K, G446S

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 S477N (AAC) T478K (AAA)

Plasmid

#212470

Purpose

Encodes SARS-CoV-2 spike S477N, T478K for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike S477N, T478K (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

S477N, T478K

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 InsertionR214-EPE

Plasmid

#212461

Purpose

Encodes SARS-CoV-2 spike R214 insertion for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike R214 insertion (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

R214 insertion

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 L452Q (CAA) F490S (TCT)

Plasmid

#212449

Purpose

Encodes SARS-CoV-2 spike L452Q, F490S for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike L452Q, F490S (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

L452Q, F490S

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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