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pnYC-NpM-SpDcp2opt_1-242-E147Q_AD

Plasmid

#148546

Purpose

Bacterial Expression of SpDcp2opt_1-242-E147Q

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpDcp2opt_1-242-E147Q (dcp2 Fission Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pILGFP4D42

Plasmid

#185840

Purpose

Testing the TEF1 promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PTEF1+[Z268]>yEGFP>TPGK1-TURA3

Expression

Yeast

Mutation

None

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP10CF71

Plasmid

#185860

Purpose

Testing the SkGAL2 promoter inserted with 4 Z268 elements using a green fluorescent protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1- PSkGAL2+[Z268]>(BamHI)yEGFP>TURA3

Expression

Yeast

Mutation

None

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP10CF72 (B9)

Plasmid

#185858

Purpose

Testing the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1- PSkGAL2M5(5*PZ4)>(BamHI)yEGFP>TURA3

Expression

Yeast

Mutation

None

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP10CF72

Plasmid

#185857

Purpose

Testing the SkGAL2 promoter inserted with 4 PZ4 elements using a green fluorescent protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1- PSkGAL2M4(4*PZ4)>(BamHI)yEGFP>TURA3

Expression

Yeast

Mutation

None

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP10E1B

Plasmid

#185856

Purpose

Testing the SkGAL2 promoter inserted with 3 PZ4 elements using a green fluorescent protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1- PSkGAL2M2(3*PZ4)>(BamHI)yEGFP>TURA3

Expression

Yeast

Mutation

None

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP10E1A

Plasmid

#185855

Purpose

Testing the SkGAL2 promoter inserted with 2 PZ4 elements using a green fluorescent protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1- PSkGAL2M1(2*PZ4)> (BamHI)yEGFP>TURA3

Expression

Yeast

Mutation

None

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP3A2 (1)

Plasmid

#185851

Purpose

Testing the TEF1 promoter appended with 2 tetracycline riboswitch using a green fluorescence protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PTEF1>2*TcRb>yEGFP>TPGK1-TURA3

Expression

Yeast

Mutation

None

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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KBB441

Plasmid

#185131

Purpose

Swi6 full length with L->A mutated in nuclear localization signal

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Tags

GFP

Expression

Yeast

Mutation

SWI6 deletion of amino acids 450-458

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9341 (pgRNA_IX-1_NatMX)

Plasmid

#161593

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IX-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9343 (pgRNA_XV-1_NatMX)

Plasmid

#161595

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XV-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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KBB345

Plasmid

#185078

Purpose

rfa3D46 truncation fused to GFP

Depositor

Ken Belanger

Article

Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

Insert

RFA3

Tags

GFP

Expression

Yeast

Mutation

Rfa3 truncation aa 1-46

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB419

Plasmid

#185089

Purpose

Swi6L-GFP expressing N-term 273 amino acids of Swi6

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Tags

GFP

Expression

Yeast

Mutation

Swi6 truncation at aa 273

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB354

Plasmid

#185082

Purpose

Swi6L-GFP expressin N-term 273 amino acids of Swi6

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Tags

GFP

Expression

Yeast

Mutation

Swi6 truncation aa 1-273

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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KBB416

Plasmid

#185087

Purpose

Swi6L-GFP with nuclear export signal mutated to alanines

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Tags

GFP

Expression

Yeast

Mutation

Swi6 nuclear export signal L to A mutations

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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KBB349

Plasmid

#185080

Purpose

rfa2D248 truncation fused to GFP

Depositor

Ken Belanger

Article

Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

Insert

RFA2

Tags

GFP

Expression

Yeast

Mutation

Rfa2 truncation aa 1-247

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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KBB353

Plasmid

#185081

Purpose

Swi6M-GFP expressing N-term 181 amino acids of Swi6

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Tags

GFP

Expression

Yeast

Mutation

Swi6 truncation aa 1-181

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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KBB484

Plasmid

#185132

Purpose

SWI6-GFP full length under control of GAL1 promotion

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Tags

GFP

Expression

Yeast

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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KBB486

Plasmid

#185133

Purpose

Full length CRM1 fused to binding domain of two-hybrid plasmid

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

CRM1

Expression

Yeast

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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KBB491

Plasmid

#185134

Purpose

Full length SWI6 fused to activation domain of two-hybrid plasmid

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Expression

Yeast

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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