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HOXB6 (human) FLAG pMSCV

Plasmid

#8534

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB6 (HOXB6 Human)

Use

Retroviral

Tags

FLAG and IRES-GFP

Expression

Mammalian

Mutation

contains improved ribosomal binding site and FLAG…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
HOXB6 (human) FLAG pMSCV

Plasmid

#8534

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB6 (HOXB6 Human)

Use

Retroviral

Tags

FLAG and IRES-GFP

Expression

Mammalian

Mutation

contains improved ribosomal binding site and FLAG…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Otx2 x2)

Plasmid

#171102

Purpose

CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Otx2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9 (Otx2 Mouse, S. pyogenes)

Use

CRISPR

Promoter

Ef1a

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Otx2 x2)

Plasmid

#171102

Purpose

CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Otx2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9 (Otx2 S. pyogenes, Mouse)

Use

CRISPR

Promoter

Ef1a

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pKG01484_pLentiX1-TRE-mRuby2-bGH-EFS-rtTA-P2A-mGL-WPRE

Plasmid

#252554

Purpose

Inducible Tet all-in-one circuit with downstream tandem syntax, lentiviral vector

Depositor

Kate Galloway

Article

Johnstone et al bioRxiv [Preprint]. 2025 Jan 19:2025.01.

Insert

rtTA-P2A-mGreenLantern

Use

Lentiviral

Expression

Mammalian

Promoter

EFS (human elongation factor 1-alpha - short/intr…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pKG01484_pLentiX1-TRE-mRuby2-bGH-EFS-rtTA-P2A-mGL-WPRE

Plasmid

#252554

Purpose

Inducible Tet all-in-one circuit with downstream tandem syntax, lentiviral vector

Depositor

Kate Galloway

Article

Johnstone et al bioRxiv [Preprint]. 2025 Jan 19:2025.01.

Insert

rtTA-P2A-mGreenLantern

Use

Lentiviral

Expression

Mammalian

Promoter

EFS (human elongation factor 1-alpha - short/intr…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG03549_pLentiX1-EFS-rtTA-P2A-mGL-WPRE-TRE-mRuby2-bGH

Plasmid

#252556

Purpose

Inducible Tet all-in-one circuit with upstream tandem syntax, lentiviral vector

Depositor

Kate Galloway

Article

Johnstone et al bioRxiv [Preprint]. 2025 Jan 19:2025.01.

Insert

rtTA-P2A-mGreenLantern

Use

Lentiviral

Expression

Mammalian

Promoter

EFS (human elongation factor 1-alpha - short/intr…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG03572_pLentiX1-(TRE-mRuby2-bGH)-cHS4.core-EFS-rtTA-P2A-mGL-WPRE

Plasmid

#252557

Purpose

Inducible Tet all-in-one circuit with divergent syntax and intergenic cHS4 core insulator, lentiviral vector

Depositor

Kate Galloway

Article

Johnstone et al bioRxiv [Preprint]. 2025 Jan 19:2025.01.

Insert

rtTA-P2A-mGreenLantern

Use

Lentiviral

Expression

Mammalian

Promoter

EFS (human elongation factor 1-alpha - short/intr…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG03576_pLentiX1-(TRE-mRuby2-bGH)-cHS4.full-EFS-rtTA-P2A-mGL-WPRE

Plasmid

#252560

Purpose

Inducible Tet all-in-one circuit with divergent syntax and intergenic cHS4 full insulator, lentiviral vector

Depositor

Kate Galloway

Article

Johnstone et al bioRxiv [Preprint]. 2025 Jan 19:2025.01.

Insert

rtTA-P2A-mGreenLantern

Use

Lentiviral

Expression

Mammalian

Promoter

EFS (human elongation factor 1-alpha - short/intr…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG03549_pLentiX1-EFS-rtTA-P2A-mGL-WPRE-TRE-mRuby2-bGH

Plasmid

#252556

Purpose

Inducible Tet all-in-one circuit with upstream tandem syntax, lentiviral vector

Depositor

Kate Galloway

Article

Johnstone et al bioRxiv [Preprint]. 2025 Jan 19:2025.01.

Insert

rtTA-P2A-mGreenLantern

Use

Lentiviral

Expression

Mammalian

Promoter

EFS (human elongation factor 1-alpha - short/intr…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG03572_pLentiX1-(TRE-mRuby2-bGH)-cHS4.core-EFS-rtTA-P2A-mGL-WPRE

Plasmid

#252557

Purpose

Inducible Tet all-in-one circuit with divergent syntax and intergenic cHS4 core insulator, lentiviral vector

Depositor

Kate Galloway

Article

Johnstone et al bioRxiv [Preprint]. 2025 Jan 19:2025.01.

Insert

rtTA-P2A-mGreenLantern

Use

Lentiviral

Expression

Mammalian

Promoter

EFS (human elongation factor 1-alpha - short/intr…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG03576_pLentiX1-(TRE-mRuby2-bGH)-cHS4.full-EFS-rtTA-P2A-mGL-WPRE

Plasmid

#252560

Purpose

Inducible Tet all-in-one circuit with divergent syntax and intergenic cHS4 full insulator, lentiviral vector

Depositor

Kate Galloway

Article

Johnstone et al bioRxiv [Preprint]. 2025 Jan 19:2025.01.

Insert

rtTA-P2A-mGreenLantern

Use

Lentiviral

Expression

Mammalian

Promoter

EFS (human elongation factor 1-alpha - short/intr…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ37, 115-133, 204-217 PPM1H

Plasmid

#207344

Purpose

Mammalian expression of HA-Δ37, 115-133, 204-217 PPM1H

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ37, Δ115-133, Δ204-217

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ37, 115-133, 204-217 PPM1H

Plasmid

#207344

Purpose

Mammalian expression of HA-Δ37, 115-133, 204-217 PPM1H

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ37, Δ115-133, Δ204-217

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Donor_N_FH_Piwi

Plasmid

#186651

Purpose

Donor plasmid to endogenously tag piwi gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS PIWI N-terminal 3xFlag-3xHA Tag Donor Plasmid (piwi Fly)

Tags

3xFlag-3xHA

Expression

Insect

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_N_FH_Piwi

Plasmid

#186651

Purpose

Donor plasmid to endogenously tag piwi gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS PIWI N-terminal 3xFlag-3xHA Tag Donor Plasmid (piwi Fly)

Tags

3xFlag-3xHA

Expression

Insect

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Cas9_ANKRD1_sgRNA2

Plasmid

#186669

Purpose

Plasmid containing Cas9 and ANKRD1 sgRNA2 , sgRNA sequence: cggtcagcttatatagct.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

ANKRD1 KO sgRNA Plasmid (ANKRD1 Human)

Expression

Mammalian

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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Cas9_ANKRD1_sgRNA2

Plasmid

#186669

Purpose

Plasmid containing Cas9 and ANKRD1 sgRNA2 , sgRNA sequence: cggtcagcttatatagct.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

ANKRD1 KO sgRNA Plasmid (ANKRD1 Human)

Expression

Mammalian

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR/D-Topo genomic A. thaliana IMPORTIN ALPHA 4

Plasmid

#175815

Purpose

pENTR plasmid with genomic sequence of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 4 (AT1G09270) without stop codon for C-terminal epitope tags

Depositor

Mark Banfield, Lennart Wirthmueller

Article

Wirthmueller et al Plant J. 2015 Jan;81(1):40-52. doi: 10.1

Insert

IMPORTIN ALPHA ISOFORM 4 (IMPA-4 Mustard Weed)

Use

Pentr plasmid for gateway cloning

Tags

none

Promoter

none

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR/D-Topo genomic A. thaliana IMPORTIN ALPHA 4

Plasmid

#175815

Purpose

pENTR plasmid with genomic sequence of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 4 (AT1G09270) without stop codon for C-terminal epitope tags

Depositor

Mark Banfield, Lennart Wirthmueller

Article

Wirthmueller et al Plant J. 2015 Jan;81(1):40-52. doi: 10.1

Insert

IMPORTIN ALPHA ISOFORM 4 (IMPA-4 Mustard Weed)

Use

Pentr plasmid for gateway cloning

Tags

none

Promoter

none

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge