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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,938 results for: emb

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Showing: 5421 - 5440 of 11938 results

  7. DCT.VV.Orf212

    Plasmid
    #12900
    Purpose
    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.
    Depositor
    Jack Bennink, Jon Yewdell
    Publication
    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VV.Orf212
    Expression
    Mammalian
    Available since
    Dec. 29, 2006
    Availability
    Academic Institutions and Nonprofits only

  11. pETSUMO2_Vitiosangium-bGSDM-thrombin

    Plasmid
    #222099
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Vitiosangium species with a thrombin protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Publication
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Vitiosangium-bGSDM-thrombin ORF
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222099-plasmid-map-sequence-id-437094

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  17. pscMS2(WTx2)-EGFP

    Plasmid
    #140258
    Purpose
    MS2CP-dependent translational repression
    Depositor
    Hirohide Saito
    Publication
    Ono et al ACS Synth Biol. 2020 Jan 17;9(1):169-174
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Two MS2CP-binding sites embedded in scaffold
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    140258-plasmid-map-sequence-id-311696

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  18. pDUAL_2_T5

    Plasmid
    #155175
    Purpose
    see pDUAL_T5: exchanged origin of replication (pBR322) and introduced bom site
    Depositor
    Bernd Nidetzky
    Publication
    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    155175-plasmid-map-sequence-id-310562

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  19. pEcRNAP5

    Plasmid
    #128939
    Purpose
    pET21a derivative encoding E. coli core RNA polymerase subunits: RpoAppx234-241-(His)10, B, C, Z
    Depositor
    Seth Darst
    Publication
    Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:
    Inserts
    RpoAppx234-241-(His)10 (rpoA Escherichia coli)
    RpoB
    RpoC
    RpoZ
    Tags
    His
    Expression
    Bacterial
    Mutation
    Residues 234-241 are substituted for a PreScissio…
    Promoter
    T7
    Available since
    Aug. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    128939-plasmid-map-sequence-id-254599

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Showing: 5421 - 5440 of 11938 results