We narrowed to 7,669 results for: Ski

1. pDY0738 pCAG-NLS-SscINT

   Plasmid

   #179113

   Purpose

   SscINT expression vector

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   pCAG-NLS-SscINT
   

   Use

   CRISPR

   Available Since

   Feb. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDY0739 pCAG-NLS-SacINT

   Plasmid

   #179114

   Purpose

   SacINT expression vector

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   pCAG-NLS-SacINT
   

   Use

   CRISPR

   Available Since

   Feb. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT3EF5a-PIK3CA H1047R

   Plasmid

   #180034

   Purpose

   Entry vector of PIK3CA H1047R for gateway cloning.

   Depositor

   Xin Chen

   Article

   Wang et al Liver Int. 2015 Dec 30. doi: 10.1111/liv

   Insert

   PIK3CA H1047R (PIK3CA Human)
   

   Use

   Entry vector

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   H1047R

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR-53BP1 1-1972 WT

   Plasmid

   #53446

   Purpose

   Recombinational donor/master vector

   Depositor

   Daniel Durocher

   Article

   Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

   Insert

   53BP1 (TP53BP1 Human)
   

   Use

   Gateway

   Mutation

   siRNA resistant and mutations A128V, T720A, A1347…

   Available Since

   April 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pDY0312 PhiBT Integrase

   Plasmid

   #179111

   Purpose

   PhiBT Integrase expression vector

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   PhiBT Integrase
   

   Use

   CRISPR

   Available Since

   Feb. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-PEmax NG

   Plasmid

   #187894

   Purpose

   All-in-one prime editor piggyBac transposon with PEmax, NG variant

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

   Insert

   SpCas9_H840A_KK_NG-MMLV-RT_co-P2A-PAC_dTK
   

   Use

   CRISPR and Synthetic Biology; Piggybac transposon

   Tags

   BPSV40 NLS and c-Myc NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. shRNA Caspase 1 (pSicoR mCherry)

   Plasmid

   #53575

   Purpose

   shRNA against human pro-caspase 1

   Depositor

   Warner Greene

   Article

   Doitsh et al Nature. 2014 Jan 23;505(7484):509-14. do

   Insert

   shRNA Caspase 1 (CASP1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   U6 for shRNA; EF1a for mCherry

   Available Since

   Aug. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pS34.EF1α<GFP-2A-Cas9-RC

   Plasmid

   #207404

   Purpose

   Cas9-RC and GFP from an EF1α promoter. High-performance knockin through DSB repair by homology-directed recombination (HDR) or homology-mediated end joining (HMEJ). [Lab plasmid ID: N213]

   Depositor

   Alexandros Poulopoulos

   Article

   Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

   Insert

   Cas9-RC
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GFP-2A

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEM-2t

   Plasmid

   #159752

   Purpose

   CRISPR/Cas9 genome editing in plants; a template for PCR-derived fragments used in the assembly of polycistronic transcripts, where sgRNAs are separated by an Arabidopsis alanine tRNA.

   Depositor

   Wolfgang Lukowitz

   Article

   Angulo et al bioRxiv

   Insert

   sgRNA
   

   Use

   CRISPR

   Available Since

   Oct. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pYEE

    Plasmid

    #159750

    Purpose

    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.

    Depositor

    Wolfgang Lukowitz

    Article

    Angulo et al bioRxiv

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6_rd6_nsgRNA(PP7)

    Plasmid

    #232434

    Purpose

    nsgRNA for PE3b correction of the rd6 mutation, contains a PP7 in the scaffold tetraloop

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    PP7-tagged nicking gRNA (PE3b) for rd6 correction driven by human U6 promoter
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYUU

    Plasmid

    #159751

    Purpose

    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.

    Depositor

    Wolfgang Lukowitz

    Article

    Angulo et al bioRxiv

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-FRT/T0-eGFPnls-53BP1 1220-1631 WT

    Plasmid

    #60814

    Purpose

    mammalian expression vector

    Depositor

    Daniel Durocher

    Article

    Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

    Insert

    53BP1 (TP53BP1 Human)
    

    Tags

    eGFP-NLS (SV40)

    Expression

    Mammalian

    Available Since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pNL-CEF-CD79A-YFP

    Plasmid

    #187004

    Purpose

    Expression of human CD79A as YFP fusion protein

    Depositor

    Hermann Eibel

    Article

    Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

    Insert

    CD79A (CD79A Human)
    

    Use

    Lentiviral

    Tags

    YFP

    Available Since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. CamKII-ADAM23-pHluorin

    Plasmid

    #185539

    Purpose

    Expresses ADAM23-pHluorin under the CamKII promoter

    Depositor

    Jaime de Juan-Sanz

    Article

    Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

    Insert

    ADAM23 (Adam23 Rat)
    

    Use

    Lentiviral

    Tags

    pHluorin

    Expression

    Mammalian

    Promoter

    CamKII

    Available Since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCW57.1 _3xHA-Ago2

    Plasmid

    #73538

    Purpose

    Inducible lentiviral expression of Ago2

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    Ago2 (Ago2 Mouse)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet ON

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6_rd12_pegRNA_(PP7-C4-Q1)

    Plasmid

    #232435

    Purpose

    pegRNA with optimized 3' modifications to correct the rd12 mutation

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    3'-PP7-tagged pegRNA for rd12 correction driven by human U6 promoter
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pU6_rd12_nsgRNA(PP7)

    Plasmid

    #232436

    Purpose

    nsgRNA for PE3b correction of the rd12 mutation, contains a PP7 in the scaffold tetraloop

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    PP7-tagged nicking gRNA (PE3b) for rd12 correction driven by human U6 promoter
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.hSynapsin.SF-Venus-iGluSnFR.A184V

    Plasmid

    #106178

    Purpose

    Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-Venus-iGluSnFR.A184V
    

    Use

    AAV

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: A184V

    Promoter

    hSynapsin

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.CAG-FLEX.SF-Venus-iGluSnFR.A184S

    Plasmid

    #106189

    Purpose

    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-Venus-iGluSnFR.A184S
    

    Use

    AAV

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S

    Promoter

    CAG

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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