We narrowed to 7,548 results for: ski

1. pWZLblasti-HA-ERK2 D321N

   Plasmid

   #53173

   Purpose

   Expresses HA tagged human ERK2 D321N from blasticidin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   ERK2 (MAPK1 Human, Mustard Weed)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D321N

   Promoter

   Available since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pCC_06 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9NG-NLS-VPR-2A-Puro-WPRE

   Plasmid

   #139091

   Purpose

   Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   dSpCas9-NG-VPR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CDKN1A (p21) targeting gRNA

   Plasmid

   #215319

   Purpose

   Expresses gRNA targeting CDKN1A (p21) and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEX CblB-SH2

   Plasmid

   #46413

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   CblB (CBLB Human)
   

   Use

   Tags

   AviTag, GST, and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 42-359

   Promoter

   Tac

   Available since

   Aug. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. FUW-TetO-Klf5

   Plasmid

   #128830

   Purpose

   dox-dependent expression of mouse Klf5

   Depositor

   Yossi Buganim

   Article

   Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

   Insert

   Klf5 (Klf5 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV.CAG.SF-Venus-iGluSnFR.S72A

   Plasmid

   #106203

   Purpose

   Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-Venus-iGluSnFR.S72A
   

   Use

   AAV

   Tags

   Expression

   Mutation

   SFGFP: T203Y, F46L, T65G, S72A GltI: S72A

   Promoter

   CAG

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV.GFAP.SF-Venus-iGluSnFR.A184S

   Plasmid

   #106195

   Purpose

   Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-Venus-iGluSnFR.A184S
   

   Use

   AAV

   Tags

   Expression

   Mutation

   SFGFP: T203Y, F46L, T65G, S72A GltI: A184S

   Promoter

   GFAP

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBABE-hygro-BAP1-C91S-HA

   Plasmid

   #154021

   Purpose

   Mammalian Expression of HA-tagged p.C91S Mutant BAP1

   Depositor

   Samuel Peña-Llopis

   Article

   Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:

   Insert

   BAP1 (BAP1 Human)
   

   Use

   Retroviral

   Tags

   Hemagglutinin (HA)

   Expression

   Mammalian

   Mutation

   p.C91S (Missense Mutation, Catalytically Inactive)

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV2-CAG-mScarlet-I-Cdt1

   Plasmid

   #191100

   Purpose

   To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

   Depositor

   Murray Blackmore, Pantelis Tsoulfas

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   mScarlet-I-Cdt1 (30-120)
   

   Use

   AAV and Synthetic Biology

   Tags

   mScarlet-I fused to residues 30-120 of human Cdt1…

   Expression

   Mammalian

   Mutation

   Optimized to human codon usage

   Promoter

   CMV enhancer and CAG

   Available since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. human rSHIP2 (18-893)

    Plasmid

    #128568

    Purpose

    Expression of human SHIP2 in bacteria

    Depositor

    Christophe Erneux

    Article

    Pesesse et al FEBS Lett. 1998 Oct 23;437(3):301-3.

    Insert

    SHIP2 (INPPL1 Human)
    

    Use

    Tags

    His Xpress (N-terminal)

    Expression

    Bacterial

    Mutation

    SHIP2 coding sequence from amino acid 18 (A) till…

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5-FRT-TO-Nsp8-Nsp7-HF_GC3opt

    Plasmid

    #157702

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp8-Nsp7-HF_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. psiCHECK2-BRCA1 3'UTR

    Plasmid

    #29477

    Depositor

    Judy Lieberman

    Article

    Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

    Insert

    BRCA1 3'UTR (BRCA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pInducer10-SAE1_sh1

    Plasmid

    #168984

    Purpose

    Expresses RFP cDNA and SAE1 shRNA 1 in mammalian cells

    Depositor

    Ji Luo

    Article

    Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    SUMO1 Activating Enzyme Subunit 1 (SAE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc promoter

    Available since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX Btk-SH2

    Plasmid

    #46412

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Btk (BTK Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 271-385

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LENTICRISPR-HNRNPD_sgRNA1

    Plasmid

    #201598

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    HNRNPD (HNRNPD Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PB-PE SpG

    Plasmid

    #179081

    Purpose

    All-in-one prime editor piggyBac transposon, SpG variant

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9 SpG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    D1135L, S1136W, G1218K, E1219Q, R1335Q, T1337R

    Promoter

    CAG

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AT5G62710 X045_pECIA2

    Plasmid

    #115130

    Purpose

    Bait vector AT5G62710 X045_pECIA2 should be used with prey vector AT5G62710 X045_pECIA14.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT5G62710 (AT5G62710 Mustard Weed)
    

    Use

    Tags

    Fc, V5 and hexahistidine tags

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein (Copper-Inducible)

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. BE3-NLS-NLS-P2A-BLAST

    Plasmid

    #135348

    Purpose

    Expression of BE3 (Base editor generation three) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    BE3-2XNLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AT1G35710 X075_pECIA2

    Plasmid

    #115098

    Purpose

    Bait vector AT1G35710 X075_pECIA2 should be used with prey vector AT1G35710 X075_pECIA14.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT1G35710 (AT1G35710 Mustard Weed)
    

    Use

    Tags

    Fc, V5 and hexahistidine tags

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein (Copper-Inducible)

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AT1G56140 X032_pECIA2

    Plasmid

    #115062

    Purpose

    Bait vector AT1G56140 X032_pECIA2 should be used with prey vector AT1G56140 X032_pECIA14.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT1G56140 (AT1G56140 Mustard Weed)
    

    Use

    Tags

    Fc, V5 and hexahistidine tags

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein (Copper-Inducible)

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge