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Addgene

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We narrowed to 23,338 results for: Nov;

Showing: 8251 - 8280 of 23338 results

  5. p-mCherry-C1-iC++_CA

    Plasmid
    #98168
    Purpose
    slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with cyan light, inactivation max with 590 nm (accelarates closure to ms). Codon optimized for mammalian expression.
    Depositor
    Peter Hegemann
    Article
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Insert
    Synthetic construct iC++_C128A gene
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    T59S, E83N, E90Q, E101S, V117R, E123S, C128A, V24…
    Promoter
    CMV (+enhancer)
    Available since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98168-plasmid-map-sequence-id-182759

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  6. AAV-TRE-NS1-dTomato

    Plasmid
    #175278
    Purpose
    AAV vector mediating inducible bicistronic expression of NS1 gene of YFV-17D and dTomato
    Depositor
    Wei Xu
    Article
    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s
    Insert
    NS1; dTomato (POLY Yellow fever virus strain 17D)
    Use
    AAV
    Tags
    dTomato (from bidirectional promoter)
    Expression
    Mutation
    Promoter
    bidirectional TRE promoter
    Available since
    Dec. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175278-plasmid-map-sequence-id-349702

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  7. pelB-MBP-BB0238-ss

    Plasmid
    #137046
    Purpose
    E. coli expression clone (T7lac promoter) for mature BB0238 (aa 21-256) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66635.2 (BB_0238 Borrelia burgdorferi B31)
    Use
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature BB0238: lacks the BB0238 signal peptide
    Promoter
    T7lac
    Available since
    April 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    137046-plasmid-map-sequence-id-292071

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  9. pLKO.1-TRC.mKO2_shB3GNT5.1

    Plasmid
    #110325
    Purpose
    TRCN0000034809 (Target GCCTATGTAATCTCCGGTGAT), silence human B3GNT5 gene and express monomeric Kusabira-Orange2
    Depositor
    Sandra Martha Gomes Dias
    Article
    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1
    Insert
    B3GNT5 (B3GNT5 Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    RNA polymerase III promoter for human U6 snRNA fo…
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110325-plasmid-map-sequence-id-213398

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  10. pLKO_HA.NWS.VSVg_NGFR

    Plasmid
    #158243
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.NWS.VSVg
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158243-plasmid-map-sequence-id-329479

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  15. pLKO_HA.FLAG.VSVg_NGFR

    Plasmid
    #158245
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.FLAG.VSVg
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158245-plasmid-map-sequence-id-327706

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  23. PB-PEmax NG

    Plasmid
    #187894
    Purpose
    All-in-one prime editor piggyBac transposon with PEmax, NG variant
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9_H840A_KK_NG-MMLV-RT_co-P2A-PAC_dTK
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    BPSV40 NLS and c-Myc NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    187894-plasmid-map-sequence-id-370740

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  24. pLKO_NWS.FLAG.VSVg_NGFR

    Plasmid
    #158233
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    NWS.FLAG.VSVg
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158233-plasmid-map-sequence-id-327650

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Showing: 8251 - 8280 of 23338 results