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Plasmid#102670PurposeTetracycline inducible expression of GFPmut2 using autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD-BirA-eCPX-AP
Plasmid#121907PurposeExpresses BirA and eCPX-APDepositorInsertsUseTags6xHisExpressionBacterialMutationPromoterT7 and pBAD promoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUF-AsCpf1-pre-gRNA
Plasmid#137849PurposeAsCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.DepositorInsertAsCpf1 and pre-gRNA cassette
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMS15: pTwinStrep-SUMO-AvTnsB
Plasmid#168148PurposeAvTnsB expression construct for protein purification.DepositorInsertAvCAST TnsB
UseTags6X His, Twin Strep and SUMOExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS16: pTwinStrep-SUMO-AvTnsC
Plasmid#168149PurposeAvTnsC expression construct for protein purification.DepositorInsertAvCAST TnsC
UseTags6X His, Twin Strep and SUMOExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS14: pTwinStrep-SUMO-AvTnsA
Plasmid#168147PurposeAvTnsA expression construct for protein purification.DepositorInsertAvCAST TnsA
UseTags6X His, Twin Strep and SUMOExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUF-LbCpf1-pre-gRNA
Plasmid#137848PurposeLbCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.DepositorInsertLbCpf1-NLS-FLAG and gRNA cassette
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZH519
Plasmid#102672PurposeTetracycline inducible expression of GFPmut2 using autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZH522
Plasmid#102675PurposeTetracycline inducible expression of GFPmut2 with constitutive TetR; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pV1539
Plasmid#111434PurposeGenedrive entry vector for Candida albicansDepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMP4516
Plasmid#107775PurposeExpression of ECFPDepositorInsertpLacZ-ECFP in pBBR1-mcs5
UseTagspLacZ-ECFPExpressionBacterialMutationPromoterpLacZAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZH518
Plasmid#102671PurposeTetracycline inducible expression of GFPmut2 using autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1+ HA-hAO7
Plasmid#137840PurposeN-terminal tagged human AO7 (aa 2-439) for expression in mammalian cellsDepositorInsertAO7 (RNF25 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
CP1300-ABEmax
Plasmid#119810PurposeA-to-G base editorDepositorInsertecTadA-ecTadA*(7.10)-nSpCas9 (CP1300)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV+ plasmid with onboard mCherry cassette
Plasmid#169741PurposeCMV+ plasmid that also encodes a separate mCherry cassette. mCherry is used to monitor plasmid dosage.DepositorInserteGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpCMV-tetO2Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-sCS4-2T
Plasmid#28250DepositorInsertsingle chain Cse4-Scm3-H4 with 2 Thrombin site
UseTagsHisExpressionBacterialMutationA thrombin cutting site between Cse4 and Scm3. An…PromoterAvailable sinceJuly 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pCK671.306
Plasmid#192641PurposeExpresses dxCas9-NG and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmRDepositorInsertJ306 scRNA
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterBBa_J23119Available sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Plas-gRNA-LEU2
Plasmid#157656PurposeGFP and gRNA tag for protein and mRNA quantification, to attache to the 3' end of the gene coding sequenceDepositorInsertGFP-gRNA
UseInsert storage (replicative in e. coli)TagsGFP-gRNAExpressionMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-DUET-1-mRhubarb719-HO
Plasmid#141200PurposeExpresses mRhubarb719 fluorescent protein and heme oxygenaseDepositorInsertsmRhubarb719
heme oxygenase
UseTags6x-HisExpressionBacterialMutationR134H, Y198S, T202Y, V203I, W309R, Q310A compared…PromoterT7Available sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only