We narrowed to 13,396 results for: mpl

1. pET28a-anticalin (E143amb)-His

   Plasmid

   #168046

   Purpose

   Expresses anticalin targeting CTLA-4 with the codon encoding E143 changed to amber codon (TAG).

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Use

   Tags

   6x Histag

   Expression

   Bacterial

   Mutation

   Glu 143 was mutated to amber codon (TAG)

   Promoter

   T7 promoter

   Available since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-anticalin (S87amb)-His

   Plasmid

   #168044

   Purpose

   Expresses anticalin targeting CTLA-4 with the codon encoding S87 changed to amber codon (TAG).

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Use

   Tags

   6x Histag

   Expression

   Bacterial

   Mutation

   Ser 87 was mutated to amber codon (TAG)

   Promoter

   T7 promoter

   Available since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a-anticalin (N116amb)-His

   Plasmid

   #168045

   Purpose

   Expresses anticalin targeting CTLA-4 with the codon encoding N116 changed to amber codon (TAG).

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Use

   Tags

   6x Histag

   Expression

   Bacterial

   Mutation

   Asn 116 was mutated to amber codon (TAG)

   Promoter

   T7 promoter

   Available since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a-anticalin (N21amb)-His

   Plasmid

   #168041

   Purpose

   Expresses anticalin targeting CTLA-4 with the codon encoding N21 changed to amber codon (TAG).

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Use

   Tags

   6x Histag

   Expression

   Bacterial

   Mutation

   Asn 21 was mutated to amber codon (TAG)

   Promoter

   T7 promoter

   Available since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a-Fgβ-anticalin-His-ybbr

   Plasmid

   #168040

   Purpose

   Expresses anticalin targeting CTLA-4 with Fgβ peptide at the N terminus and 6x-His tag and ybbr tag at the C terminus

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Use

   Tags

   6x Histag, Fgβ peptide, and ybbr tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a(+)-HisTim12_CtoA

   Plasmid

   #170281

   Purpose

   Expresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

   Depositor

   Paul Schanda

   Article

   Weinhaupl et al Structure. 2021 May 3. pii: S0969-2126(2

   Insert

   yeast Tim12 (TIM12 Budding Yeast)
   

   Use

   Tags

   TEV-cleavable N-terminal His tag (on backbone)

   Expression

   Bacterial

   Mutation

   mutated two non-conserved Cys to Ala

   Promoter

   Available since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-anticalin (I55amb)-His

   Plasmid

   #168042

   Purpose

   Expresses anticalin targeting CTLA-4 with the codon encoding I55 changed to amber codon (TAG).

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Use

   Tags

   6x Histag

   Expression

   Bacterial

   Mutation

   Ile 55 was mutated to amber codon (TAG)

   Promoter

   T7 promoter

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS406 ATG1-ATG7-3'UTR

   Plasmid

   #166847

   Purpose

   Expresses Atg1 with ATG7 3'UTR in yeasts.

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Mol Cell. 2019 Jan 17;73(2):314-324.e4.

   Insert

   ATG1 (ATG1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ATG1 Promoter

   Available since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR-D-TOPO-kcnt2a

   Plasmid

   #164963

   Purpose

   Zebrafish kcnt2a gene CDS

   Depositor

   GuangJun Zhang

   Article

   Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

   Insert

   kcnt2a (kcnt2 Zebrafish)
   

   Use

   Pcr cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-Coh-ddFLN4-ELP(MV7E2)3-His-ybbR

    Plasmid

    #157672

    Purpose

    Expression of Coh (type III from Ruminococcus flavefaciens) with ddFLN4, ELP linker, His6x tag, and ybbR Tag.

    Depositor

    Michael Nash

    Article

    Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

    Insert

    Cohesin (R.f) with ddFLN4, ELP, Histag and ybbR tag
    

    Use

    Tags

    ddFLN4, ELP, Histag and ybbR tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFA-DAB1

    Plasmid

    #162691

    Purpose

    Expresses DabAB1 inorganic carbon transport complex

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    DabAB1 inorganic carbon transport complex
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCFJ1972 - [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)

    Plasmid

    #159844

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCFJ2301 - [1-2] ENTR - Fluor - ce-mMaple3(1 intron, syntrons(3), no_atg, no_stop)

    Plasmid

    #159865

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-mMaple3(1 intron, syntrons(3), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSWD95Y14D

    Plasmid

    #159098

    Purpose

    CckA FRET Sensor with cyclic-di-GMP point mutant

    Depositor

    Seth Childers

    Article

    Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

    Insert

    CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691(Y514D), Linker SNVTRHRSAT, mRuby3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Mutated Y514 to D514

    Promoter

    Xylose

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSWD95D293A

    Plasmid

    #159099

    Purpose

    CckA FRET Sensor with DITE motif mutant

    Depositor

    Seth Childers

    Article

    Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

    Insert

    CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691 (D293A), Linker SNVTRHRSAT, mRuby3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    CckA D293 mutated to A293 and V199I on mRuby3 (P…

    Promoter

    Xylose

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET_4E2H.210

    Plasmid

    #155212

    Purpose

    Expresses 4E2H.210 de novo protein in E.coli

    Depositor

    Bruno Correia

    Article

    Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

    Insert

    4E2H.210
    

    Use

    Tags

    6x His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEBTetBl-CLIP-Centrin3

    Plasmid

    #136855

    Purpose

    Mammalian expression of the centrosomal protein Centrin3 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Centrin3
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEBTetDBl-CLIP-Centrin3

    Plasmid

    #136856

    Purpose

    Mammalian expression of the centrosomal protein Centrin3 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Centrin3
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEBTetDBl-CLIP-FOP

    Plasmid

    #136846

    Purpose

    Mammalian expression of the centrosomal protein FGFR N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-FGFR
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEBTetDBl-CLIP-POC5

    Plasmid

    #136836

    Purpose

    Mammalian expression of the centrosomal protein POC5 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-POC5 (POC5 Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge