We narrowed to 677 results for: Bacillus

1. pTTCC

   Plasmid

   #164264

   Purpose

   Expresses a thermostable Cas9 enzyme and gRNA in Thermus thermophilus

   Depositor

   Gudmundur Hreggvidsson

   Article

   Adalsteinsson et al Sci Rep. 2021 May 5;11(1):9586. doi: 10.

   Inserts

   Cas9
   gRNA scaffold
   

   Expression

   Bacterial

   Promoter

   Pcel and trc

   Available Since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21a(+)-Bst LF-6xHis

   Plasmid

   #159148

   Purpose

   expresses His-tagged large fragment of Bst polymerase

   Depositor

   Andrea Pauli

   Article

   Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d

   Insert

   large fragment of Geobacillus stearothermophilus DNA polymerase I
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Sept. 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pThermoCas9_ctrl

   Plasmid

   #100981

   Purpose

   Expresses ThermoCas9 and its sgRNA module

   Depositor

   John van der Oost

   Article

   Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:

   Inserts

   Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
   ThermoCas9 single guide RNA expressing module
   

   Tags

   None

   Expression

   Bacterial

   Promoter

   B. coagulans DSM 1 pta promoter and B. smithii xy…

   Available Since

   Nov. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pThermoCas9i_ctrl

   Plasmid

   #100982

   Purpose

   Expresses the catalytically inactive version of ThermoCas9 (Thermo(d)Cas9) and its sgRNA module

   Depositor

   John van der Oost

   Article

   Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:

   Inserts

   Catalytically inactive variant of the Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
   ThermoCas9 single guide RNA expressing module
   

   Tags

   None

   Expression

   Bacterial

   Mutation

   changed aspartate 8 to alanine and histidine 582 …

   Promoter

   B. coagulans DSM 1 pta promoter and B. smithii xy…

   Available Since

   Nov. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGLU_81

   Plasmid

   #225782

   Purpose

   RS421 library variant 13401

   Depositor

   Nili Ostrov

   Insert

   13401_[abr]
   

   Use

   Synthetic Biology

   Mutation

   N/A

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGLU_99

   Plasmid

   #225812

   Purpose

   RS421 library variant 13401

   Depositor

   Nili Ostrov

   Insert

   13401_[Himar]
   

   Use

   Synthetic Biology

   Mutation

   N/A

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGLU_117

   Plasmid

   #225797

   Purpose

   RS421 library variant 13401

   Depositor

   Nili Ostrov

   Insert

   13401_[Tn5]
   

   Use

   Synthetic Biology

   Mutation

   N/A

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL0_169 [pTHT15 ORI]

   Plasmid

   #210688

   Purpose

   Level 0 part

   Depositor

   Charlie Gilbert, Cultivarium Tools, Nili Ostrov

   Insert

   pTHT15 ORI
   

   Use

   Synthetic Biology

   Available Since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKAR2-Br512g3.1

   Plasmid

   #171200

   Purpose

   Expresses Br512g3.1 polymerase in E.coli

   Depositor

   Andrew Ellington

   Article

   Bhadra et al medRxiv 2022.08.09.22278605

   Insert

   Engineered Bst DNA Polymerase I Large Fragment
   

   Tags

   8x His tag; Villin Headpiece 47 amino acid (vHP47…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKAR2-Br512g3.2

    Plasmid

    #171201

    Purpose

    Expresses Br512g3.2 polymerase in E.coli

    Depositor

    Andrew Ellington

    Article

    Bhadra et al medRxiv 2022.08.09.22278605

    Insert

    Engineered Bst DNA Polymerase I Large Fragment
    

    Tags

    8x His tag; Villin Headpiece 47 amino acid (vHP47…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-28a(+)-FPPS

    Plasmid

    #108952

    Purpose

    heterologous expression of FPPS of G. stearothermophilus in E. coli

    Depositor

    Frank Schulz

    Article

    Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

    Insert

    Farnesyldiphosphat synthase (FPPS)
    

    Tags

    His6-Tag

    Expression

    Bacterial

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. S7SZ03

    Plasmid

    #163199

    Purpose

    Inducible expression of UniProt identifier S7SZ03

    Depositor

    Martin Engqvist

    Article

    Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

    Insert

    S7SZ03
    

    Tags

    10xHis and T7

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCB591

    Plasmid

    #141096

    Purpose

    E. coli-Lactobacilli shuttle vector for recombineering template cloning

    Depositor

    Chase Beisel

    Article

    Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS-ermGFP

    Plasmid

    #27172

    Depositor

    Michela Lizier

    Article

    Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

    Insert

    erythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5
    

    Expression

    Bacterial

    Mutation

    N/A

    Available Since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTec_mCherry

    Plasmid

    #229145

    Purpose

    Promoter strength evaluation of Ptec

    Depositor

    Shrikrishnan Sankaran

    Article

    Blanch-Asensio et al Microb Cell Fact. 2024 Feb 7;23(1):42. d

    Insert

    mCherry
    

    Expression

    Bacterial

    Mutation

    No

    Promoter

    Ptec

    Available Since

    Jan. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSL4164 (pEffector_IscB,ωRNA)

    Plasmid

    #210874

    Purpose

    Expresses GstIscB and native ωRNA from constitutive promoters

    Depositor

    Samuel H. Sternberg

    Article

    Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

    Insert

    GstIscB
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-AsCas12f1-v5.1+T1-empty

    Plasmid

    #204977

    Purpose

    AsCas12f1-v5.1+T1-based Human cell genome editing

    Depositor

    Quanjiang Ji

    Article

    Wu et al Nature Catalysis (2023) 6, 695–709

    Type

    Empty backbone

    Use

    CRISPR

    Mutation

    N70Q/K103R/A104R/S118A/D364R

    Promoter

    PCMV,U6

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSL4369 (pEffector_TnpB2,ωRNA)

    Plasmid

    #210871

    Purpose

    Expresses GstTnpB2 and native ωRNA from constitutive promoters

    Depositor

    Samuel H. Sternberg

    Article

    Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

    Insert

    GstTnpB2
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSL4672 (pEffector_TnpB3,ωRNA)

    Plasmid

    #210872

    Purpose

    Expresses GstTnpB3 and native ωRNA from constitutive promoters

    Depositor

    Samuel H. Sternberg

    Article

    Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

    Insert

    GstTnpB3
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSL4673 (pEffector_TnpB4,ωRNA)

    Plasmid

    #210873

    Purpose

    Expresses GstTnpB4 and native ωRNA from constitutive promoters

    Depositor

    Samuel H. Sternberg

    Article

    Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

    Insert

    GstTnpB4
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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