We narrowed to 14,352 results for: NTS

1. pAF0034 Ef1a-Kp03-2a-EGFP

   Plasmid

   #193461

   Purpose

   Expression of Kp03 recombinase from Ef1a promoter

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Kp03
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSP1673

   Plasmid

   #65769

   Purpose

   Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7 (x2)

   Available since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAF0036 Ef1a-Si74-2a-EGFP

   Plasmid

   #193463

   Purpose

   Expression of Si74 recombinase from Ef1a promoter

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Si74
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-mOXT-hM3Dq-mCherry-WPRE

   Plasmid

   #70717

   Purpose

   Expresses DREADDs Gq receptor under oxytocin promoter control

   Depositor

   Daniel Geschwind

   Article

   Penagarikano et al Sci Transl Med. 2015 Jan 21;7(271):271ra

   Insert

   hM3Dq-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   mouse oxytocin

   Available since

   Jan. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAF0432 Ef1a-Dn29-2a-EGFP

   Plasmid

   #193459

   Purpose

   Expression of Dn29 recombinase from Ef1a promoter

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Dn29
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAF0692 Ef1a-Dn29_attD-Puro-p2a-mCherry

   Plasmid

   #193465

   Purpose

   Dn29 Donor with promoter for mcherry and puro expression

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Dn29_attP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAF0035 Ef1a-Nm60-2a-EGFP

   Plasmid

   #193464

   Purpose

   Expression of Nm60 recombinase from Ef1a promoter

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Nm60
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAF0084 Ef1a-Cp36-2a-EGFP

   Plasmid

   #193462

   Purpose

   Expression of Cp36 recombinase from Ef1a promoter

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Cp36
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GFP-BAF

   Plasmid

   #227442

   Purpose

   Mammalian expression of GFP-BAF

   Depositor

   David Pellman

   Article

   Umbreit et al Science. 2020 Apr 17;368(6488):eaba0712.

   Insert

   Barrier-to-autointegration factor (BANF1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJT371 PB ITR and Cp36 attD pEF Puro-2a-mCherry donor

    Plasmid

    #184951

    Purpose

    Donor for both PiggyBac and Cp36 Recombinase

    Depositor

    Ami Bhatt, Lacra Bintu, Patrick Hsu, Michael Bassik

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    PuroR, P2A, mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SNAP-BAF

    Plasmid

    #227443

    Purpose

    Mammalian expression of SNAP-BAF

    Depositor

    David Pellman

    Article

    Umbreit et al Science. 2020 Apr 17;368(6488):eaba0712.

    Insert

    Barrier-to-autointegration factor (BANF1 Human)
    

    Use

    Lentiviral

    Tags

    SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MSP469

    Plasmid

    #65771

    Purpose

    Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135V, R1335Q, and T1337R mutations in Cas9

    Promoter

    CMV & T7

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MSP680

    Plasmid

    #65772

    Purpose

    Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135E, R1335Q, and T1337R mutations in Cas9

    Promoter

    CMV & T7

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. BPK2101

    Plasmid

    #65770

    Purpose

    Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized SaCas9, and SaCas9 gRNA
    

    Use

    CRISPR

    Tags

    NLS-3xFlag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (x2)

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSP2133

    Plasmid

    #72249

    Purpose

    Human expression plasmid for SpCas9-HF4 variant: CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 HF4(Y450A/N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    Y450A, N497A, R661A, Q695A, and Q926A

    Promoter

    CMV

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAF0362 Ef1a-Cp36_attP-Puro-p2a-mCherry donor

    Plasmid

    #193469

    Purpose

    Cp36 Donor with promoter for mcherry and puro expression

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Cp36_attP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJP-CoV2leader-nLuc-TSS

    Plasmid

    #175430

    Purpose

    Expresses nLuc with 5' leader of CoV2 in mammalian cells.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nLUC plus 5' CoV2 leader
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MSP2135

    Plasmid

    #72248

    Purpose

    Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A, and D1135E

    Promoter

    CMV

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX6P1-human full-length LIC1

    Plasmid

    #74597

    Purpose

    used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    full length human dynein light intermediate chain 1 (DYNC1LI1 Human)
    

    Use

    Tags

    N-terminal GST and Strep II

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pECE M2-SNX17 WT

    Plasmid

    #69811

    Purpose

    Expresses M2-SNX17 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    Sorting nexin-17 (SNX17 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SV40 early promoter

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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