We narrowed to 23,338 results for: Nov;

1. pSpCas9-Puro HLAG-2B-sgRNA

   Plasmid

   #182552

   Purpose

   Cas9 from S.pyogenes with 2A-Puro, and the 2B-sgRNA to targeting exon 2 of human HLA-G gene

   Depositor

   Santiago Miriuka

   Article

   Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

   Insert

   hSpCas9-2A-Puro-HLAG-2B-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiCRISPR v2-Nr4a1sgRNA1

   Plasmid

   #160945

   Purpose

   Guide RNA 1 to generate Nr4a1 knockout by CRISPR

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Nr4a1 gRNA 1 (Nr4a1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEGFP-RNF138-delta 18-58

   Plasmid

   #78921

   Purpose

   Mammalian expression of RNF138 with delta 18-58 and an EGFP fusion

   Depositor

   Michael Hendzel

   Article

   Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

   Insert

   RNF138 (RNF138 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   delta 18-58

   Promoter

   CMV

   Available since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pJC-elav1.8kb-TALE1-VP64-P10

   Plasmid

   #104609

   Purpose

   Expresses TALE1 under the control of a 1.8kb elav enhancer element

   Depositor

   Tudor Fulga

   Article

   Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

   Insert

   1.8kb elav enhancer element (elav Fly)
   

   Use

   TALEN

   Tags

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   Jan. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Mouse aB crystallin 250bp promoter/AcGFP

   Plasmid

   #98102

   Purpose

   Mouse aB crystallin 250bp promoter segment driving GFP expression

   Depositor

   Mason Posner

   Article

   Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

   Insert

   Alpha B-crystallin promoter 250 bp fragment, Mus musculus (Cryab Mouse)
   

   Use

   Gfp expressing

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Alpha B-crystallin promoter 250 bp fragment (-259…

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 (-) hYAP 1-2gamma

   Plasmid

   #124145

   Purpose

   Protein expression for funtional studies of cell growth promotion

   Depositor

   Marius Sudol, Megan Finch-Edmondson

   Article

   Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   None (Wild Type)

   Promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HOXB6 (human) FLAG pMSCV

   Plasmid

   #8534

   Depositor

   Corey Largman

   Article

   Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

   Insert

   HOXB6 (HOXB6 Human)
   

   Use

   Retroviral

   Tags

   FLAG and IRES-GFP

   Expression

   Mammalian

   Mutation

   contains improved ribosomal binding site and FLAG…

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

8. PB-PE VQR

   Plasmid

   #162797

   Purpose

   All-in-one prime editor piggyBac transposon, VQR variant

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

   Insert

   SpCas9_H840A_VQR-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
   

   Use

   CRISPR and Synthetic Biology; Piggybac transposon

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   D1135V, R1335Q, T1337R

   Promoter

   CAG

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJC-mhc2.4kb-TALE3-VP64-P10

   Plasmid

   #104611

   Purpose

   Expresses TALE3 under the control of a 2.4kb mhc enhancer element

   Depositor

   Tudor Fulga

   Article

   Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

   Insert

   2.4kb mhc enhancer element (Mhc Fly)
   

   Use

   TALEN

   Tags

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   Jan. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLVX-CMV-XRCC1-gRNA res-Neo

    Plasmid

    #176149

    Purpose

    XRCC1 with dual PAM resistance to XRCC1 gRNA1 and XRCC1 gRNA2 & a neomycin/G418 resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    X-ray repair cross complementing 1 (XRCC1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Dual PAM resistance to XRCC1 gRNA 1 and XRCC1 gRN…

    Promoter

    CMV

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-PE NG

    Plasmid

    #174554

    Purpose

    All-in-one prime editor piggyBac transposon, NG variant

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9 NG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    L1111R, G1218R, E1219F, A1322R, R1335V, T1337R

    Promoter

    CAG

    Available since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSUPER-retro-puro-Smad2

    Plasmid

    #89827

    Purpose

    Knockdown human Smad2 expression in human mammalian cells

    Depositor

    Shengyu Yang

    Article

    Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

    Insert

    SMAD2 shRNA (SMAD2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Mouse aB crystallin 800bp promoter/AcGFP

    Plasmid

    #98103

    Purpose

    Mouse aB crystallin 800bp promoter segment driving GFP expression

    Depositor

    Mason Posner

    Article

    Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

    Insert

    Alpha B-crystallin promoter 800 bp fragment, Mus musculus (Cryab Mouse)
    

    Use

    Gfp expressing

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Alpha B-crystallin promoter 800 bp fragment (-833…

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV_Donor/Reporter-COL4A5

    Plasmid

    #130280

    Purpose

    COL4A5 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A5 donor template

    Depositor

    Silvestro Conticello

    Article

    Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

    Insert

    mCherry-eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. sgSf3b1(T1)

    Plasmid

    #90424

    Purpose

    induces dsDNA break within mouse Sf3b1 gene for subsequent homology-directed recombination and coding sequence mutagenesis at codon 700

    Depositor

    Alex Minella

    Article

    Gupta et al Nucleic Acids Res. 2018 Nov 20. pii: 519

    Insert

    mus Sf3b1 gRNA (Sf3b1 Mouse)
    

    Use

    CRISPR; Guiderna encoding for gene editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLKO_S.C.V5_NGFR

    Plasmid

    #158331

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    S.C.V5

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. FLAG-EglN2 wt-pBabe-Puro

    Plasmid

    #22704

    Depositor

    William Kaelin

    Article

    Zhang et al Cancer Cell. 2009 Nov 6. 16(5):413-24.

    Insert

    Egg laying Nine - 2 (EglN2) (EGLN2 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    wt cDNA

    Promoter

    Available since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJEP312-pAAV-CMV-SaCas9-P2A-HAFLAGHA-KASH-pA

    Plasmid

    #113689

    Purpose

    SaCas9 driven by CMV. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    SaCas9 (NEWENTRY )
    

    Use

    AAV and CRISPR

    Tags

    Flag, HAx2, KASH, and NLS

    Expression

    Mutation

    Promoter

    Cytomegalo Virus(CMV)

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMSCVpuro-F-Gcn5-NLSm

    Plasmid

    #63708

    Purpose

    retroviral expression of cytoplasm-localized mouse Gcn5 in mammalian cells

    Depositor

    Kai Ge

    Article

    Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

    Insert

    Gcn5 (Kat2a Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    mutated K425, K427 and K438 to R (arginine)

    Promoter

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.1 (-) hYAP 1-1alpha

    Plasmid

    #124139

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Marius Sudol, Megan Finch-Edmondson

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None (Wild Type)

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. R989-X79-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) M188L

    Plasmid

    #159692

    Purpose

    Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) M188L

    Depositor

    Dominic Esposito

    Article

    Procter et al Protein Expr Purif. 2018 Nov;151:99-105.

    Insert

    His6-MBP-tev-G-Hs.KRAS4b(1-188) M188L (KRAS Human)
    

    Use

    Tags

    His6-MBP-tev

    Expression

    Insect

    Mutation

    M188L

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. HOXB5 (human) FLAG-MSCV

    Plasmid

    #8532

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB5 (HOXB5 Human)
    

    Use

    Retroviral

    Tags

    FLAG and IRES-GFP

    Expression

    Mammalian

    Mutation

    contains improved ribosomal binding site and FLAG…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

23. pNIA-CEN-mVenus-FLAG-LANS1-Set2

    Plasmid

    #122003

    Purpose

    mVenus-FLAG-LANS-Set2: mVenus- and FLAG-tagged LANS1-Set2 for light control of Set2 localization in yeast

    Depositor

    Brian Kuhlman

    Article

    Lerner et al Genome Res. 2020 Nov;30(11):1605-1617. d

    Insert

    mVenus-FLAG-LANS1-Set2 (SET2 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    mVenus-FLAG

    Expression

    Yeast

    Mutation

    Set2 point mutations K538G, K539S, R549G, K550S, …

    Promoter

    ADH1

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLKO_V5.AU1.FLAG_NGFR

    Plasmid

    #158240

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    V5.AU1.FLAG

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLKO.1-TRC.mKO2_shGLS

    Plasmid

    #110319

    Purpose

    shGLS (Target CAACTGGCCAAATTCAGTC), silence human GLS gene and express monomeric Kusabira-Orange2

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    GLS Glutaminase (GLS Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEGFP-RNF138-delta 86-105

    Plasmid

    #78922

    Purpose

    Mammalian expression of RNF138 with delta 86-105 and an EGFP fusion

    Depositor

    Michael Hendzel

    Article

    Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

    Insert

    RNF138 (RNF138 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    delta 86-105

    Promoter

    CMV

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

27. pEGFP-RNF138-delta 158-180

    Plasmid

    #78923

    Purpose

    Mammalian expression of RNF138 with delta 158-180 and an EGFP fusion

    Depositor

    Michael Hendzel

    Article

    Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

    Insert

    RNF138 (RNF138 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    delta 159-180

    Promoter

    CMV

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

28. pEGFP-RNF138-delta 189-215

    Plasmid

    #78924

    Purpose

    Mammalian expression of RNF138 with delta 189-215 and an EGFP fusion

    Depositor

    Michael Hendzel

    Article

    Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

    Insert

    RNF138 (RNF138 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    delta 189-215

    Promoter

    CMV

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

29. pLKO_HSV.Ollas.FLAG_NGFR

    Plasmid

    #158270

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    HSV.Ollas.FLAG

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3-HA-BHLHE40(S243A/E248A)

    Plasmid

    #110155

    Purpose

    Expression of human BHLHE40 (S243A/E248A) in mammalian cells

    Depositor

    Daniele Guardavaccaro

    Article

    Kim et al Mol Cell Biol. 2014 Nov 15;34(22):4177-8

    Insert

    BHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S243A/E248A

    Promoter

    CMV

    Available since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge