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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,646 results for: dykddddk

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Showing: 5621 - 5640 of 8646 results

  2. pGG369

    Plasmid
    #165606
    Purpose
    Vector for expression of ω-dCas9 and sgRNA in E. coli: lacUV2-ω-1xFLAG-dCas9(Wt PID)-1xNLS-3xHA-1xNLS and UV5-BsaI-sgRNA (BsaI sites in place of the spacer)
    Depositor
    Marcus Noyes
    Publication
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    ω-1xFLAG-dCas9(PAM-interacting domain and overall coding scheme from Wt SpCas9)-1xNLS-3xHA-1xNLS and BsaI-sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    1x FLAG, 3x HA, Omega (ω) subunit of RNA polymera…
    Expression
    Bacterial
    Mutation
    Recoding of BsaI site in the AmpR cassette, two B…
    Promoter
    lacUV2 driving ω-dCas9 and UV5 driving sgRNA
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    165606-plasmid-map-sequence-id-330964

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  8. GP805

    Plasmid
    #164545
    Purpose
    Expresses Smo-FLAG K348, 360, 434, 444, 448, 543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R
    Depositor
    Gregory Pazour
    Publication
    Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151
    Insert
    Smoothened K348, 360, 434, 444, 448, 543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R (Smo Mouse)
    Use
    Lentiviral
    Tags
    FLAG
    Mutation
    All 21 cytoplamic Lysines of Smoothened changed t…
    Promoter
    GgCryD1
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    164545-plasmid-map-sequence-id-325341

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  9. GP804

    Plasmid
    #164548
    Purpose
    Expresses Smo-FLAG K543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R
    Depositor
    Gregory Pazour
    Publication
    Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151
    Insert
    Smoothened K543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R (Smo Mouse)
    Use
    Lentiviral
    Tags
    FLAG
    Mutation
    All sixteen Lysines in cytoplasmic C-terminal tai…
    Promoter
    GgCryD1
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    164548-plasmid-map-sequence-id-325344

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  14. pMSCV_puro_41589

    Plasmid
    #111632
    Purpose
    Complementation of Dicer_KO mESCs with mutated human DICER isoform
    Depositor
    Constance Ciaudo
    Insert
    FLAG-hsDicer (K70A) (DICER1 Human)
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    K70A
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    111632-plasmid-map-sequence-id-217766

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  17. RG6-BAP1_E11-G312G

    Plasmid
    #154026
    Purpose
    Bichromatic Fluorescent Splicing Reporter Minigene for Mutant BAP1 Exon 11 c.936T>G, p.G312G (Synonymous Mutation)
    Depositor
    Samuel Peña-Llopis
    Publication
    Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:
    Insert
    BAP1 Exon 11 Fused to DsRed1 and eGFP (BAP1 Human)
    Use
    Splicing reporter
    Tags
    DsRed1, FLAG, SV40 NLS, and eGFP
    Expression
    Mammalian
    Mutation
    BAP1 c.936T>G, p.G312G
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    154026-plasmid-map-sequence-id-307080

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  19. DT043A

    Plasmid
    #159404
    Purpose
    Constitutive superfolder GFP (sfGFP) expression, controlled under T7 bacteriophage promoter
    Depositor
    Sheref Mansy
    Publication
    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea
    Insert
    pr.T7-sfGFP
    Use
    Synthetic Biology
    Tags
    FLAG
    Expression
    Bacterial
    Promoter
    T7 bacteriophage promoter
    Available since
    Feb. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    159404-plasmid-map-sequence-id-324320

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Showing: 5621 - 5640 of 8646 results