We narrowed to 13,186 results for: Mpl;

1. psiCHECK2-miR-309

   Plasmid

   #135697

   Purpose

   Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-309)

   Depositor

   Florian Karreth

   Insert

   miR-309 reverse complement
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. psiCHECK2-miR-543

   Plasmid

   #135699

   Purpose

   Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-543)

   Depositor

   Florian Karreth

   Insert

   miR-543 reverse complement
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. psiCHECK2-miR-653

   Plasmid

   #135700

   Purpose

   Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-653)

   Depositor

   Florian Karreth

   Insert

   miR-653 reverse complement
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. psiCHECK2-miR-134

   Plasmid

   #135701

   Purpose

   Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-134)

   Depositor

   Florian Karreth

   Insert

   miR-134 reverse complement
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. psiCHECK2-miR-125

   Plasmid

   #135702

   Purpose

   Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-125)

   Depositor

   Florian Karreth

   Insert

   miR-125 reverse complement
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. psiCHECK2-miR-133

   Plasmid

   #135704

   Purpose

   Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-133)

   Depositor

   Florian Karreth

   Insert

   miR-133 reverse complement
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. psiCHECK2-miR-497

   Plasmid

   #135706

   Purpose

   Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-497)

   Depositor

   Florian Karreth

   Insert

   miR-497 reverse complement
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. psiCHECK2-miR-183-5p

   Plasmid

   #135719

   Purpose

   Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-183-5p)

   Depositor

   Florian Karreth

   Insert

   miR-183-5p reverse complement
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. psiCHECK2-miR-194-5p

   Plasmid

   #135720

   Purpose

   Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-194-5p)

   Depositor

   Florian Karreth

   Insert

   miR-194-5p reverse complement
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPD088 ZF43x7-Spaced_EYFP

    Plasmid

    #138880

    Purpose

    EYFP reporter vector for the ZF43x7-Spaced promoter

    Depositor

    Joshua Leonard

    Insert

    ZF43x7-Spaced
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF43x7-Spaced

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPD068 ZF43x5-Spaced_EYFP

    Plasmid

    #138878

    Purpose

    EYFP reporter vector for the ZF43x5-Spaced promoter

    Depositor

    Joshua Leonard

    Insert

    ZF43x5-Spaced
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF43x5-Spaced

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. T-B18

    Bacterial Strain

    #159446

    Purpose

    E. coli BW25113 ΔfrmA, harboring plasmid Addgene plasmid # 129104, was adaptively evolved to grow efficiently on threonine and perform significant biosynthesis while employing methanol-derived carbon

    Depositor

    Maciek R. Antoniewicz, Eleftherios Terry Papoutsakis

    Article

    Har et al Appl Microbiol Biotechnol. 2021 Jan;105(

    Bacterial Resistance

    Ampicillin and kanamycin

    Available since

    Aug. 18, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

13. BW-Para

    Bacterial Strain

    #172602

    Purpose

    The BW-Para E. coli strain is used to screen the functions of chimeric AraC/XylS transcription activators using beta-galactosidase assays.

    Depositor

    Liskin Swint-Kruse

    Article

    Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

    Bacterial Resistance

    None

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

14. pCS2+MCS-P2A-sfGFP

    Plasmid

    #74668

    Purpose

    Plasmid for expression of wild-type or mutant cDNA fragments (mutation reporter) or complete cDNAs in frame with superfolderGFP.

    Depositor

    Jason Berman

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pOpen-P45

    Plasmid

    #165570

    Purpose

    Significantly improves maximum amplicon size and PCR yields for Family B polymerases like Pfu or Phusion. Comparable to ArchaeMaxx/PfuTurbo.

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    P45
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 23, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. mCherry-MannII-N-10

    Plasmid

    #55074

    Purpose

    Localization: Golgi Complex, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    MannII
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    aa 1-112 of MannII

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pCMVlac-dirTopo-AU1-aGFP

    Plasmid

    #61971

    Purpose

    A directional cloning vector of PCR-amplified cDNAs for mammalian expression

    Depositor

    Hiroshi Udo

    Inserts

    lacZ alpha
    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    It contains silent mutations

    Promoter

    Available since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC-dirTopo-P2A-aGFP

    Plasmid

    #61974

    Purpose

    A directional cloning vector of PCR-amplified cDNAs for mammalian expression

    Depositor

    Hiroshi Udo

    Inserts

    lacZ alpha
    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    It contains silent mutations

    Promoter

    Available since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC-dirTopo-AU1-aGFP

    Plasmid

    #61973

    Purpose

    A directional cloning vector of PCR-amplified cDNAs for mammalian expression

    Depositor

    Hiroshi Udo

    Inserts

    lacZ alpha
    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    It contains silent mutations

    Promoter

    Available since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIAeBlue

    Plasmid

    #185891

    Purpose

    In vivo gene amplification (HamAmp) of AeBlue expression module at RPL25 locus

    Depositor

    Claudia Vickers

    Insert

    PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>AeBlue>TPGK1- PBTS1> RPL25(partial; Arm2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pIEforRed

    Plasmid

    #185892

    Purpose

    In vivo gene amplification (HamAmp) of EforRed expression module at RPL25 locus

    Depositor

    Claudia Vickers

    Insert

    PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>EforRed>TPGK1-PBTS1> RPL25(partial; Arm2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mTurquoise-MannII-N-10

    Plasmid

    #55569

    Purpose

    Localization: Golgi Complex, Excitation: 434, Emission: 474

    Depositor

    Michael Davidson

    Insert

    MannII
    

    Use

    Tags

    mTurquoise

    Expression

    Mammalian

    Mutation

    aa 1-112 of MannII

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

23. pOpen-dbh

    Plasmid

    #165513

    Purpose

    Thermostable gamma-family lesion-bypass DNA Polymerase that efficiently synthesizes DNA across a variety of DNA template lesions.

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    Sulfolobus DNA Polymerase IV
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. CAMP no 3'UTR

    Plasmid

    #129561

    Purpose

    cytomplasmic marker without terminator (less integration)

    Depositor

    Inaki Ruiz-Trillo

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCR2.1-InC

    Plasmid

    #83959

    Purpose

    Contains a synthetic insert to serve as internal control DNA template for a Salmonella Real-Time PCR assay.

    Depositor

    Kai-Shun Chen

    Insert

    Internal Control sequence
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMVlac-dirTopo-P2A-aGFP

    Plasmid

    #61972

    Purpose

    A directional cloning vector of PCR-amplified cDNAs for mammalian expression

    Depositor

    Hiroshi Udo

    Inserts

    lacZ alpha
    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    It contains silent mutations

    Promoter

    Available since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMCMV3

    Plasmid

    #85711

    Purpose

    Expression vector employing the mouse CMV major immediate-early promoter enhancer (MIEP); there is a small polylinker (PstI, SmaI, XbaI, SalI ,HpaI) followed by the SV40 polyA signal sequences

    Depositor

    Ulrich Koszinowski, Martin Messerle

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MIEPmouse CMV MIEP

    Available since

    April 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TC9pAB

    Plasmid

    #71310

    Purpose

    used as a template for in vitro transcription of Cas9 mRNA after linearizing with BbsI

    Depositor

    Karl Degenhardt

    Insert

    Cas9
    

    Use

    In vitro transcription

    Tags

    NLS

    Expression

    Mutation

    Promoter

    T7

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPD148 ZF43x10-Spaced-Far_EYFP

    Plasmid

    #138894

    Purpose

    EYFP reporter vector for the ZF43x10-Spaced-Far promoter

    Depositor

    Joshua Leonard

    Insert

    ZF43x10-Spaced-Far
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF43x10-Spaced-Far

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPD146 ZF43x6-Spaced-Far_EYFP

    Plasmid

    #138892

    Purpose

    EYFP reporter vector for the ZF43x6-Spaced-Far promoter

    Depositor

    Joshua Leonard

    Insert

    ZF43x6-Spaced-Far
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF43x6-Spaced-Far

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pPD138 ZF43x10-Spaced-Mid_EYFP

    Plasmid

    #138888

    Purpose

    EYFP reporter vector for the ZF43x10-Spaced-Mid promoter

    Depositor

    Joshua Leonard

    Insert

    ZF43x10-Spaced-Mid
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF43x10-Spaced-Mid

    Available since

    Jan. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pPD136 ZF43x6-Spaced-Mid_EYFP

    Plasmid

    #138886

    Purpose

    EYFP reporter vector for the ZF43x6-Spaced-Mid promoter

    Depositor

    Joshua Leonard

    Insert

    ZF43x6-Spaced-Mid
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF43x6-Spaced-Mid

    Available since

    Jan. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. mEmerald-TGNP-N-10

    Plasmid

    #54279

    Purpose

    Localization: Golgi Complex, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    TGNP (TGOLN2 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pOpen-T4gene30

    Plasmid

    #165554

    Purpose

    Ligase derived from T4 bacteriophage expressed in the early stage of lytic development. Has been implicated in T4 DNA synthesis and genetic recombination. May also play a role in T4 DNA repair.

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    T4-DNA Ligase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pDD400

    Plasmid

    #137024

    Purpose

    SapTrap mNG-GLO donor

    Depositor

    Daniel Dickinson, Bob Goldstein

    Insert

    C. elegans GLO-optimized mNeonGreen
    

    Use

    CRISPR; Saptrap donor for cloning repair templat…

    Tags

    C. elegans GLO-optimized mNeonGreen

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV_CbH_SIO_SyGCaMP8s_p2a_mRuby

    Plasmid

    #205280

    Purpose

    Synaptic activity can be imaged with Synaptophysin GCaMP, while simultaneously, a red fluorescence structural marker can be employed to provide a visual reference for the cellular architecture.

    Depositor

    Matthias Prigge

    Insert

    GCaMP8s
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pINER5

    Plasmid

    #185895

    Purpose

    In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).

    Depositor

    Claudia Vickers

    Insert

    PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PSk.GAL2>YFAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 25, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pLXIN2-RFP

    Plasmid

    #99205

    Purpose

    RFP expressing bicistronic retroviral vector

    Depositor

    Saroj Mathupala

    Insert

    Red fluorescent protein
    

    Use

    Retroviral; Bicistronic retroviral expression vec…

    Tags

    Expression

    Mammalian

    Mutation

    The dsRed cDNA was PCR amplified off AddGene plas…

    Promoter

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMS051

    Plasmid

    #137025

    Purpose

    SapTrap mScarlet-I-GLO donor

    Depositor

    Daniel Dickinson, Bob Goldstein

    Insert

    C. elegans GLO-optimized mScarlet
    

    Use

    CRISPR; Saptrap donor for cloning repair templat…

    Tags

    C. elegans GLO-optimized mScarlet

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDD346

    Plasmid

    #133311

    Purpose

    SapTrap mNG-C1 donor

    Depositor

    Daniel Dickinson

    Insert

    C. elegans codon-optimized mNeonGreen
    

    Use

    CRISPR; Saptrap donor for cloning repair templat…

    Tags

    C. elegans codon-optimized mNeonGreen

    Expression

    Worm

    Mutation

    Promoter

    None

    Available since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge