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p349_DG

Plasmid

#234679

Purpose

Golden gate domesticated pET28b with His10-GST-3C

Depositor

Matthew Wohlever

Publication

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-GST-3C

Expression

Bacterial

Mutation

No

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p351_DG

Plasmid

#234681

Purpose

Golden gate domesticated pET28b with His10-mNeongreen-3C

Depositor

Matthew Wohlever

Publication

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-mNeongreen-3C

Expression

Bacterial

Mutation

No

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p358_DG

Plasmid

#234701

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-bdNEDD8

Depositor

Matthew Wohlever

Publication

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-bdNEDD8

Expression

Bacterial

Mutation

No

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p348_DG

Plasmid

#234678

Purpose

Golden gate domesticated pET28b with His10-MBP-3C

Depositor

Matthew Wohlever

Publication

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-MBP-3C

Expression

Bacterial

Mutation

No

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p350_DG

Plasmid

#234680

Purpose

Golden gate domesticated pET28b with His10-bdNEDD8

Depositor

Matthew Wohlever

Publication

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-bdNEDD8

Expression

Bacterial

Mutation

No

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p355_DG

Plasmid

#234698

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-Thrombin

Depositor

Matthew Wohlever

Publication

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-Thrombin

Expression

Bacterial

Mutation

No

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pOPTG-Tau-RING

Plasmid

#233691

Purpose

Expression of Tau-RING protein in bacteria

Depositor

Leo James

Publication

Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

Insert

pOPTG

Expression

Bacterial

Available since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_FLAG3

Plasmid

#223896

Purpose

4G-cloning donor for C-terminal FLAG3 tag

Depositor

Stephan Gruber

Publication

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

FLAG3

Use

Golden-gate donor

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT20

Plasmid

#200990

Purpose

pL0V-Lv2-pUC18R6KT-miniTn7-GG-Esp3I sites for Antb modules, ApR

Depositor

Philip Poole

Publication

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Type

Empty backbone

Expression

Bacterial

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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AA295

Plasmid

#215986

Purpose

Fragmid fragment: (N' terminus) SV40 Nuclear Localization Signal

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has service

Cloning Grade DNA

Insert

Start; SV40NLS_v1.19

Use

Fragment

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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AA345

Plasmid

#216031

Purpose

Fragmid fragment: (Cas protein) Cas13d

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has service

Cloning Grade DNA

Insert

Cas13d_v1.1 [Dj]

Use

CRISPR; Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA068

Plasmid

#215994

Purpose

Fragmid fragment: (Selection) puromycin resistance

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has service

Cloning Grade DNA

Insert

Start; PuroR_v1.2

Use

Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA179

Plasmid

#215939

Purpose

Fragmid fragment: (guide cassette) short sequence with no known function

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has service

Cloning Grade DNA

Insert

Filler

Use

CRISPR; Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA082

Plasmid

#215997

Purpose

Fragmid fragment: (Selection) fluorescent reporter

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has service

Cloning Grade DNA

Insert

Start; mTagBFP2_v1.1

Use

Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRDA_832

Plasmid

#216085

Purpose

Cas9 [Sp] knockout targeting CD47, positive control

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD47 guide

Use

CRISPR and Lentiviral; Positive controls

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRDA_836

Plasmid

#216088

Purpose

Cas9 [Sp] knockout targeting CD55, positive control

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD55 guide

Use

CRISPR and Lentiviral; Positive controls

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRDB_118

Plasmid

#216089

Purpose

Cas9 [Sp] CRISPRa targeting CD47, positive control

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD47 guide

Use

CRISPR and Lentiviral; Positive controls

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA012

Plasmid

#215974

Purpose

Fragmid fragment: (N' terminus) Bipartite Nuclear Localization Signal

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has service

Cloning Grade DNA

Insert

Start; BPNLS_v1.1

Use

Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pKE014

Plasmid

#207908

Purpose

GG entry for zCas9i expression

Depositor

Alexis Maizel

Publication

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

SpCas9_introns_2xNLS-Pea3A(T) term

Use

CRISPR

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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OmpT

Plasmid

#213137

Purpose

OmpT_native membrane expression

Depositor

Sebastian Hiller

Publication

Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

Insert

OmpT

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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