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pTK162_mCherry-4.1G-hardened

Plasmid

#46361

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

4.1G (EPB41L2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Modified for siRNA resistance to gcTccTcaTttCgaAc…

Promoter

CMV

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pAN19-3xFLAG-ccvTIR1-NOSt

Plasmid

#108546

Purpose

Cloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminator

Depositor

Keiko Torii

Article

Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

Insert

TRANSPORT INHIBITOR RESPONSE 1 [F79G] fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)

Use

Cloning vector

Tags

3xFLAG

Mutation

Changed F79 to G

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pAN19-3xFLAG-ccvTIR1-NOSt

Plasmid

#108546

Purpose

Cloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminator

Depositor

Keiko Torii

Article

Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

Insert

TRANSPORT INHIBITOR RESPONSE 1 [F79G] fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)

Use

Cloning vector

Tags

3xFLAG

Mutation

Changed F79 to G

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/GFP-GIGYF1

Plasmid

#141188

Purpose

Expresses GFP-GIGYF1 in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

Insert

GIGYF1 (GIGYF1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/GFP-GIGYF1

Plasmid

#141188

Purpose

Expresses GFP-GIGYF1 in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

Insert

GIGYF1 (GIGYF1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR/D-Topo genomic A. thaliana IMPORTIN ALPHA 1

Plasmid

#175814

Purpose

pENTR plasmid with genomic sequence of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 1 (AT3G06720) without stop codon for C-terminal epitope tags

Depositor

Mark Banfield, Lennart Wirthmueller

Article

Wirthmueller et al Plant J. 2015 Jan;81(1):40-52. doi: 10.1

Insert

IMPORTIN ALPHA ISOFORM 1 (IMPA-1 Mustard Weed)

Use

Pentr plasmid for gateway cloning

Tags

none

Promoter

none

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR/D-Topo genomic A. thaliana IMPORTIN ALPHA 1

Plasmid

#175814

Purpose

pENTR plasmid with genomic sequence of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 1 (AT3G06720) without stop codon for C-terminal epitope tags

Depositor

Mark Banfield, Lennart Wirthmueller

Article

Wirthmueller et al Plant J. 2015 Jan;81(1):40-52. doi: 10.1

Insert

IMPORTIN ALPHA ISOFORM 1 (IMPA-1 Mustard Weed)

Use

Pentr plasmid for gateway cloning

Tags

none

Promoter

none

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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TFORF0751

Plasmid

#143729

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

HIVEP1 (HIVEP1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF0751

Plasmid

#143729

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

HIVEP1 (HIVEP1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF2205

Plasmid

#144004

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

POU2F3 (POU2F3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF2205

Plasmid

#144004

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

POU2F3 (POU2F3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF0052

Plasmid

#141497

Purpose

Lentiviral vector for overexpressing the MAZ transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MAZ (MAZ Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF0052

Plasmid

#141497

Purpose

Lentiviral vector for overexpressing the MAZ transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MAZ (MAZ Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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R

Plasmid

#167170

Purpose

Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant in Sf9 cells

Depositor

Susanne Dobler

Article

Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

Inserts

ATP1A1-R
ATP1B1

Expression

Bacterial and Insect

Promoter

P10 and PH

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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R

Plasmid

#167170

Purpose

Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant in Sf9 cells

Depositor

Susanne Dobler

Article

Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

Inserts

ATP1A1-R
ATP1B1

Expression

Bacterial and Insect

Promoter

P10 and PH

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pGEMHE:hTRAAK(Q258K)

Plasmid

#133077

Purpose

X. laevis expression vector. It will generate the human TRAAK channel

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1), Q258K (Kcnk2 Mouse)

Mutation

Q258K

Promoter

T7

Available Since

Oct. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMHE:hTRAAK(Q258K)

Plasmid

#133077

Purpose

X. laevis expression vector. It will generate the human TRAAK channel

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1), Q258K (Kcnk2 Mouse)

Mutation

Q258K

Promoter

T7

Available Since

Oct. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-UNC93A_STOP

Plasmid

#161448

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

UNC93A (UNC93A Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-UNC93A_STOP

Plasmid

#161448

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

UNC93A (UNC93A Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC25A16_STOP

Plasmid

#161145

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A16 (SLC25A16 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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