We narrowed to 59,919 results for: %s

1. Td-CBEmax

   Plasmid

   #196600

   Purpose

   Mammalian expression of Td-CBEmax

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

   Insert

   bpNLS-TadA-8e (N46L/E27R)-32aa linker-nSpCas9-bpNLS-P2A-UGI-bpNLS
   

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   TadA-8e (N46L/E27R); S. pyogenes Cas9 (D10A)

   Available Since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. px458-CCND1

   Plasmid

   #172630

   Purpose

   Expresses a gRNA against CCND1 and Cas9 from S. pyogenes with 2A-EGFP

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   gRNA against CCND1 (CCND1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO-PHB-8

   Plasmid

   #122233

   Purpose

   Expresses shRNA targeting the coding sequence of human PHB

   Depositor

   Alex Kentsis

   Article

   Ortiz et al JCI Insight. 2019 Aug 8;4(15). pii: 1270

   Insert

   shRNA targeting PHB (see partial depositor seq) (PHB1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Aug. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO-PHB-6

   Plasmid

   #122232

   Purpose

   Expresses shRNA targeting the coding sequence of human PHB

   Depositor

   Alex Kentsis

   Article

   Ortiz et al JCI Insight. 2019 Aug 8;4(15). pii: 1270

   Insert

   shRNA targeting PHB (see partial depositor seq) (PHB1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Aug. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOPINVHH_A8_His

   Plasmid

   #201718

   Purpose

   Expression of nanobody

   Depositor

   Ray Owens

   Article

   Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

   Insert

   VHH_A8 (S lama glama (llama))
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Available Since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOPINVHH_A10-5_His

   Plasmid

   #201720

   Purpose

   Expression of nanobody

   Depositor

   Ray Owens

   Article

   Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

   Insert

   VHH_A10-5 (S lama glama (llama))
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Available Since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOPINVHH_B5-5_His

   Plasmid

   #201721

   Purpose

   Expression of nanobody

   Depositor

   Ray Owens

   Article

   Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

   Insert

   VHH B5-5 (S lama glama (llama))
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Available Since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. MAC-C Sars-CoV-2 S_L241-243del

   Plasmid

   #194841

   Purpose

   Sars-CoV-2 S_L241-243del in MAC-C destination vector

   Depositor

   Markku Varjosalo

   Article

   Varjosalo et al 10 May 2024, Research Square

   Insert

   S_L241-243del (S Sars-CoV-2)
   

   Tags

   MAC-C

   Expression

   Mammalian

   Mutation

   L241-243del

   Available Since

   May 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. px458-AMBRA1 #3

   Plasmid

   #172607

   Purpose

   Expresses gRNA #3 against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   gRNA against AMBRA1 (AMBRA1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. px458-AMBRA1 (optimized for N-terminal knock-in)

    Plasmid

    #172608

    Purpose

    Expresses a gRNA against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP. This gRNA is suitable for the N-terminal knock-in of AMBRA1.

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    gRNA against AMBRA1 (AMBRA1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-U6-sasgRNACMV- mCherry-WPREpA

    Plasmid

    #217015

    Purpose

    S. aureus sgRNAs backbone along with mCherry expression

    Depositor

    Nadav Ahituv

    Article

    Matharu et al Science. 2018 Dec 13. pii: science.aau06

    Insert

    mCherry
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. TPI-PTC160

    Plasmid

    #130698

    Purpose

    Expresses TPI-PTC160 NMD reporter (It has a Premature termination codon (PTC) at 160th Amino acid)

    Depositor

    Marvin Tanenbaum

    Article

    Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

    Insert

    TPI-PTC160 (TPI1 Human)
    

    Expression

    Mammalian

    Mutation

    PTC160

    Promoter

    Pcmv

    Available Since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTpal-fdc

    Plasmid

    #78288

    Purpose

    Expresses A. thaliana PAL2 and S. cerevisiae FDC1 in E. coli

    Depositor

    David Nielsen

    Article

    McKenna et al Biotechnol J. 2013 Dec;8(12):1465-75. do

    Inserts

    PAL2 (PAL2 Mustard Weed)
    FDC1 (FDC1 Budding Yeast)
    

    Expression

    Bacterial

    Mutation

    *see below

    Promoter

    trc

    Available Since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFPV25.1_rpsM_mCerulean3

    Plasmid

    #124904

    Purpose

    rpsM promoter (S. typhimurium) driving mCerulean3 (codon-optimized for S. typhimurium)

    Depositor

    Markus Covert

    Article

    Lane et al Cell Syst. 2019 Mar 27;8(3):183-196.e4.

    Insert

    rpsM promoter and mCerulean3
    

    Expression

    Bacterial

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAW-AlphaLobe

    Plasmid

    #113255

    Purpose

    E. coli expression vector for His-MBP-Cas9 Alpha-Helical Lobe

    Depositor

    Jennifer Doudna

    Article

    Wright et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    S. pyogenes Cas9 alpha-helical lobe
    

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    residues 56–714; See paper for description

    Promoter

    T7

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. TPI-PTC1

    Plasmid

    #130699

    Purpose

    Expresses TPI-PTC1 NMD reporter (It has a Premature termination codon (PTC) at First Amino acid)

    Depositor

    Marvin Tanenbaum

    Article

    Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

    Insert

    TPI-PTC1 (TPI1 Human)
    

    Expression

    Mammalian

    Mutation

    PTC1

    Promoter

    Pcmv

    Available Since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL3-U6-sgRNA_SRD5A1-mcherry

    Plasmid

    #159780

    Purpose

    S. pyogenes sgRNA collocated with pegRNA targeting human SRD5A1 gene

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

    Insert

    spacer of sgRNA targeting SRD5A1 gene (SRD5A1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-Sga-Cas9

    Plasmid

    #227005

    Purpose

    Mammalian expression of Nuclease active S. gallolyticus Cas9

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-Sin-Cas9

    Plasmid

    #227006

    Purpose

    Mammalian expression of Nuclease active S. iniae Cas9

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-Spa-Cas9

    Plasmid

    #227007

    Purpose

    Mammalian expression of Nuclease active S. parasanguinis Cas9

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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