We narrowed to 9,872 results for: otos

1. Skylan-S-N1

   Plasmid

   #129737

   Purpose

   A green photo-switchable fluorescent protein for super-resolution optical fluctuation imaging (SOFI)

   Depositor

   Pingyong Xu

   Article

   Zhang et al ACS Nano. 2015 Mar 24;9(3):2659-67. doi:

   Type

   Empty backbone

   Use

   Tags

   Skylan-S

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. wt HIV-1 integrase in pET15b

   Plasmid

   #61668

   Purpose

   wild type HIV-1 integrase in pET-15b

   Depositor

   Robert Craigie

   Article

   Jenkins et al J Biol Chem. 1996 Mar 29;271(13):7712-8.

   Insert

   wild type HIV-1 integrase
   

   Use

   Tags

   His

   Expression

   Mutation

   Promoter

   T7 promotor

   Available since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

3. tol2-4xnr UAS-PA Rac1-mCherry

   Plasmid

   #41878

   Depositor

   Anna Huttenlocher

   Insert

   photoactivatable Rac1 (RAC1 Human)
   

   Use

   Zebrafish expression

   Tags

   6x His and mCherry

   Expression

   Mutation

   Rac1 starts at I4 and contains mutations Q61L, E9…

   Promoter

   Available since

   Oct. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNUT1

   Plasmid

   #175746

   Purpose

   Single-component photo-inducible (LOV2-based) nucleolus-targeting tool 1

   Depositor

   Yubin Zhou

   Article

   Tan et al Nucleic Acids Res. 2022 Mar 23. pii: 655

   Insert

   mCherry-LOV2-NoLS (HIV Rev transactivating protein)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-aDTX-lumitoxin

   Plasmid

   #51686

   Purpose

   Expression vector for aDTX-lumitoxin

   Depositor

   Edward Boyden

   Article

   Schmidt et al Nat Commun. 2014 Jan 10;5:3019. doi: 10.

   Insert

   aDTX-lumitoxin
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCZGY2842

   Plasmid

   #87762

   Purpose

   Punc-25-PH::miniSOG, expression of membrane targeted PH-miniSOG in GABAergic motor neurons.

   Depositor

   Andrew Chisholm

   Article

   Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

   Insert

   Punc-25-PH-miniSOG
   

   Use

   Tags

   PH

   Expression

   Worm

   Mutation

   Promoter

   Punc-25

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSF-KpAGO207-1251

   Plasmid

   #117854

   Purpose

   Expressed KpAGO 207-1251 in E. coli under IPTG-inducible promotor

   Depositor

   Kotaro Nakanishi, Dinshaw Patel

   Article

   Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

   Insert

   Argonaute
   

   Use

   Tags

   6xHis-SUMO encoded in vector

   Expression

   Bacterial

   Mutation

   missing first 206 amino acid residues

   Promoter

   T7

   Available since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. cp196V1.2

   Plasmid

   #52100

   Purpose

   A improved cpmCherry variant

   Depositor

   Robert Campbell

   Article

   Carlson et al Protein Eng Des Sel. 2013 Dec;26(12):763

   Insert

   cp196V1.2
   

   Use

   Tags

   6 His tag

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD promotor

   Available since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1-DTXK-lumitoxin

   Plasmid

   #51691

   Purpose

   Expression vector for DTXK-lumitoxin

   Depositor

   Edward Boyden

   Article

   Schmidt et al Nat Commun. 2014 Jan 10;5:3019. doi: 10.

   Insert

   DTXK-lumitoxin
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. mEos2-Talin-N-22

    Plasmid

    #57420

    Purpose

    Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

    Insert

    Talin
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. poRBS

    Plasmid

    #154125

    Purpose

    Expresses orthogonal rRNAs operon in bacterial cells

    Depositor

    Alexander Mankin

    Article

    Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

    Insert

    engineered bacterial rRNAs operon under control of pL promotor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. mV-CrPH-LOV1_227

    Plasmid

    #58687

    Purpose

    Expresses mVenus-fusion protein of CrPH-LOV1 domain (Chlamydomonas reinhardtii Phototropin)

    Depositor

    Harald Janovjak

    Article

    Grusch et al EMBO J. 2014 Jul 1. pii: e201387695.

    Insert

    mV-CrPH-LOV1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. mV-AtPH1-LOV2_229

    Plasmid

    #58688

    Purpose

    Expresses mVenus-fusion protein of AtPH1-LOV2 domain (Arabidopsis thaliana Phototropin 1)

    Depositor

    Harald Janovjak

    Article

    Grusch et al EMBO J. 2014 Jul 1. pii: e201387695.

    Insert

    mV-AtPH1-LOV2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pICSL30010

    Plasmid

    #50305

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS no stop codon, mEOS2 photoactivatable fluorescent protein (Lobophyllia hemprichii)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. PBP-E3-HA

    Plasmid

    #236182

    Purpose

    Other half of photoactivatable GFE3. When co-expressed with tagRFP-GPHN.FingR-PhoCl (Plasmid #236183), ablates inhibitory synapses after exposure to 400 nm light.

    Depositor

    Don Arnold

    Article

    Bareghamyan et al eLife. 2025 Apr 29;13:RP103757. doi: 10.

    Insert

    PBP-E3-HA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. Hv_HPT:pCSRM2C:Gus_introns

    Plasmid

    #215191

    Purpose

    Evaluating guard cell specific promotors in Barley

    Depositor

    Penny Hundleby

    Article

    Nguyen et al Plant Direct. 2025 Feb 12;9(2):e70045. d

    Insert

    Scream2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    BdSCRM2

    Available since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19::ZmUBI-AvrPWT4

    Plasmid

    #237516

    Purpose

    Protoplast expression

    Depositor

    Gitta Coaker

    Article

    Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

    Insert

    AvrPwt4
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC19::ZmUBI-VirPWT4

    Plasmid

    #237517

    Purpose

    Protoplast expression

    Depositor

    Gitta Coaker

    Article

    Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

    Insert

    VirPwt4
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC19::ZmUBI-RWT4Ca

    Plasmid

    #237509

    Purpose

    Protoplast expression

    Depositor

    Gitta Coaker

    Article

    Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

    Insert

    Rwt4Ca
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC19::ZmUBI-RWT4Cs

    Plasmid

    #237510

    Purpose

    Protoplast expression

    Depositor

    Gitta Coaker

    Article

    Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

    Insert

    Rwt4Cs
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. 6x Halo-EGFP- 2x PACT

    Plasmid

    #107265

    Purpose

    6x Halo-EGFP- 2x PACT construct downstream of T7 promotor for in vitro transcription

    Depositor

    Michael Lampson

    Article

    Akera et al Science. 2017 Nov 3;358(6363):668-672. d

    Insert

    6x Halo-EGFP- 2x PACT
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pEVOL-pAzF

    Plasmid

    #31186

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG

    Depositor

    Peter Schultz

    Article

    Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7

    Insert

    M.j. p-azidohenylalanine RS (2 copies +tRNA)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32T, E107N, D158P, I159L, L162Q, D286R

    Promoter

    Available since

    Dec. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA3-10His-mStayGold (E138D)

    Plasmid

    #211363

    Purpose

    Mammalian expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Contains a N-terminal 10xHis tag.

    Depositor

    Mohan Balasubramanian

    Article

    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

    Insert

    mStayGold
    

    Use

    Tags

    10xHis-tag

    Expression

    Mammalian

    Mutation

    E138D

    Promoter

    CMV

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. ClopHensorN

    Plasmid

    #50758

    Purpose

    Mammalian expression vector expressing the ClopHensorN chloride and proton sensor

    Depositor

    Colin Akerman

    Article

    Raimondo et al Front Cell Neurosci. 2013 Nov 13;7:202.

    Insert

    ClopHensorN
    

    Use

    Tags

    Strep-II

    Expression

    Mammalian

    Mutation

    E2GFP is EGFP-T203Y

    Promoter

    CAG

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLX-sgRNA

    Plasmid

    #50662

    Purpose

    Lentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)

    Depositor

    David Sabatini, Eric Lander

    Article

    Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

    Insert

    sgAAVS1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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26. pUC57-CmYLCV

    Plasmid

    #205245

    Purpose

    For plant prime editing in wheat plants or monocotyledons protoplasts

    Depositor

    Zong Yuan

    Article

    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

    Insert

    CmYLCV-csy4_binding_site-esgRNA-tevopreQ1-csy4_binding_site
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CmYLCV

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

    Plasmid

    #175570

    Purpose

    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    XbaI site added upstream of U6 promotor. G to C …

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSECRETS-A

    Plasmid

    #196986

    Purpose

    For SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).

    Depositor

    Eric Josephs

    Article

    Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pltetO1

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMU-MITr

    Plasmid

    #61198

    Purpose

    Fluorescent reporter for motochondria ScCOX4sp-mCherry expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    ScCOX4sp-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGEMHE-vhhGFP4-hIgG1-Fc

    Plasmid

    #105576

    Purpose

    T7 promotor drives in vitro mRNA transcription of GFP nanobody-Fc antibody domain fusion

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    Human IgG1 Fc-domain fused with GFP nanobody (IGHG1 Human)
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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