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AiP20171 - pAAV-hsA2-AiE0086m-minRho-iCre(R297T)-BGHpA

Plasmid

#230807

Purpose

AiE0086m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minRho

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12214 - pAAV-ProB12-SYFP2-WPRE3-BGHpA (Alias: CN2214)

Plasmid

#208161

Purpose

AAV vector for SYFP2 expression in brain astrocytes

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

Beta Globin minimal promoter

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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AiP11712 - pAAV-hsA2-AiE0219h-minRho-SYFP2-WPRE3-BGHpA (Alias: CN1712)

Plasmid

#230101

Purpose

AiE0219h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minRho

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVLINKv1-pCALM1-Cre-3'-KIF1A-FLAG

Plasmid

#244343

Purpose

3' AAVLINK plasmid for KIF1A expression

Depositor

Zhonghua Lu

Article

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

KIF1A (KIF1A Human)

Use

AAV

Tags

FLAG

Promoter

pCALM1

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVLINKv1-pCALM1-Cre-3'-CHD1-FLAG

Plasmid

#244347

Purpose

3' AAVLINK plasmid for CHD1 expression

Depositor

Zhonghua Lu

Article

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

CHD1 (CHD1 Human)

Use

AAV

Tags

FLAG

Promoter

pCALM1

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-mGFAP(ABC1D)-2pabPAC(F198Y)

Plasmid

#234548

Purpose

to express a version with reduced constitutive activity (thanks to point mutation F198Y) of the optogenetic tool 2pabPAC, which increases cAMP levels when stimulated by blue light, in astrocytes

Depositor

Hajime Hirase

Article

Vittani et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

2pabPAC(F198Y)

Use

AAV

Mutation

F198Y

Promoter

mGFAP(ABC1D)

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-SspB-EGFP-VP16-P2A-FLAG-Gal4DBD-CapN-SsrA-CapC-HA

Plasmid

#213536

Purpose

Expresses the split transcription factors in mouse liver

Depositor

Wenjing Wang

Article

Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

Insert

SspB-EGFP-VP16-P2A-FLAG-Gal4DBD-CapN-SsrA-CapC-HA-HA

Use

AAV

Tags

EGFP and HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR30(sapI)-CMV-intron-hfCas13d-pA

Plasmid

#195865

Purpose

To express hfCas13d

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

high fidelity (hf) version of CasRx (hfCas13d)

Use

AAV

Mutation

Yes: GTGGAATACATTACCAACGTGGTGTACGTG

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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AAV8-hSyn-flex-miR30-eGFP-shNts

Plasmid

#132717

Purpose

Encodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Nts gene which encodes neurotensin

Depositor

Robert Messing

Article

Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

Insert

GFP-shNts (Nts Rat)

Use

AAV

Promoter

Synapsin 1

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-ON-ArgiNLS-oScarlet

Plasmid

#252221

Purpose

Flp-dependent expression of a single-cell discriminating version of oScarlet fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

oScarlet

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-(n1)StayGold(c4)-P2A-mCherry

Plasmid

#229603

Purpose

Expresses (n1)StayGold(c4) and mCherry in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

(n1)StayGold(c4)

Use

AAV

Expression

Mammalian

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mBaoJin-P2A-FusionRed

Plasmid

#229572

Purpose

Expresses mBaoJin and FusionRed in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

mBaoJin

Use

AAV

Expression

Mammalian

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mNeonGreen-P2A-mCherry

Plasmid

#229600

Purpose

Expresses mNeonGreen and mCherry in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

mNeonGreen

Use

AAV

Expression

Mammalian

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-CasRx-P2A-mCherry-pA

Plasmid

#233025

Purpose

To Express HA tagged CasRX and mcherry from the mammalian EFS promoter. The CasRx and mCherry are separated by a P2A site

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx/mCherry

Use

AAV

Tags

mCherry

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon-EYFP

Plasmid

#231926

Purpose

Cre-on/Flp-on EYFP under the Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

eYFP with introns

Use

AAV

Promoter

Ef1a

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-EYFP

Plasmid

#231927

Purpose

Cre-off/Flp-on EYFP under the Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

eYFP with introns

Use

AAV

Promoter

Ef1a

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEX-ArgiNLS-EGFP

Plasmid

#220601

Purpose

Cre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

EGFP

Use

AAV and Cre/Lox

Tags

ArgiNLS

Expression

Mammalian

Promoter

CAG

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEX-ArgiNLS-AausFP1

Plasmid

#220602

Purpose

Cre-dependent expression of a single-cell discriminating version of AausFP1 fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

AausFP1

Use

AAV and Cre/Lox

Tags

ArgiNLS

Expression

Mammalian

Promoter

CAG

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-GRAB_g5-HT2m

Plasmid

#208722

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT2m in neurons in the presence of Cre recombinase

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT2m

Use

AAV

Promoter

EF1a

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-GRAB_g5-HT3.0mut

Plasmid

#208724

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT3.0mut in neurons in the presence of Cre recombinase

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT3.0mut

Use

AAV

Promoter

EF1a

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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