We narrowed to 11,785 results for: emb

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pL0_EF-H4

Plasmid

#202178

Purpose

Level 0 plasmid containing a terminator sequence from gene 34971 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

H4 terminator

Use

Synthetic Biology

Mutation

None

Available since

Aug. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-H4

Plasmid

#202122

Purpose

Level 0 plasmid containing the promoter from gene 34971 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

H4 promoter

Use

Synthetic Biology

Mutation

None

Available since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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U6-TM2Emp-6T_iBlue

Plasmid

#200235

Purpose

Backbone for cloning short RNA triggers that activate iSBH-sgRNAs

Depositor

Tatjana Sauka-Spengler

Publication

Pelea et al bioRxiv 2023.05.08.539738

Type

Empty backbone

Expression

Mammalian

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pQE80l B-CN-B

Plasmid

#196477

Purpose

Protein expression of SpyCatcher-ELP-CarHCN-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCN

Depositor

Fei Sun

Publication

Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1

Insert

B-CN-B

Expression

Bacterial

Promoter

T7

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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BASIC_SEVA_5a6.10

Plasmid

#178934

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Publication

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Available since

Feb. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pRB30

Plasmid

#165480

Purpose

pTriEx based vector for protein expression in mammalian cells for targets fused with C-terminal Flag and His

Depositor

Renato Bruni

Publication

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

TEV-FLAG-10x His

Expression

Mammalian

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-665 (attR4-attR2)

Plasmid

#161885

Purpose

Multisite Gateway (attR4-attR2) vector for lentivirus production

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Lentiviral

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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Auxiliary Δ

Plasmid

#153355

Purpose

Provides End-to-End linker 4Δ for level 2 cloning

Depositor

Naomi Nakayama

Publication

Andreou et al bioRxiv, March 31, 2021

Insert

End-to-End linker 4Δ

Use

Synthetic Biology

Available since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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Auxiliary Γ

Plasmid

#153354

Purpose

Provides End-to-End linker 4Γ for level 2 cloning

Depositor

Naomi Nakayama

Publication

Andreou et al bioRxiv, March 31, 2021

Insert

End-to-End linker 4Γ

Use

Synthetic Biology

Available since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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Auxiliary B

Plasmid

#153353

Purpose

Provides End-to-End linker 4B for level 2 cloning

Depositor

Naomi Nakayama

Publication

Andreou et al bioRxiv, March 31, 2021

Insert

End-to-End linker 4B

Use

Synthetic Biology

Available since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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Auxiliary 3

Plasmid

#153351

Purpose

Provides Middle-to-End linker 3 for level 2 cloning

Depositor

Naomi Nakayama

Publication

Andreou et al bioRxiv, March 31, 2021

Insert

Middle-to-End linker 3

Use

Synthetic Biology

Available since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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Auxiliary 1

Plasmid

#153349

Purpose

Provides Middle-to-End linker 1 for level 2 cloning

Depositor

Naomi Nakayama

Publication

Andreou et al bioRxiv, March 31, 2021

Insert

Middle-to-End linker 1

Use

Synthetic Biology

Available since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pTK746

Plasmid

#114712

Purpose

Express NuMA(1-2115ΔNLS)5A-3-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Publication

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(1-2115ΔNLS)5A-3-RFP-Nano

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pTK713

Plasmid

#114699

Purpose

Express NuMA(1-2115ΔNLS)-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Publication

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(1-2115ΔNLS)-RFP-Nano

Available since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMXLK_pFa

Plasmid

#114229

Purpose

POC12367, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type p-a

Depositor

Christopher A. O'Callaghan

Publication

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMXLK_dFq

Plasmid

#114237

Purpose

POC12377, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type d-q

Depositor

Christopher A. O'Callaghan

Publication

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMXLP_pFa

Plasmid

#114239

Purpose

POC12379, MetClo assembly vector with p15A replication origin and ampicillin resistance for BsaI-based MetClo, adaptor sequence type p-a

Depositor

Christopher A. O'Callaghan

Publication

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pTU1-D-yckABD

Plasmid

#112790

Purpose

Downstream homology arm, Pair of HAs spans yckA and yckB, which share homology with ABC transporters in B. subtilis

Depositor

Chris French

Publication

Wicke et al bioRxiv 185108

Insert

yckABD

Expression

Bacterial

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pTU1-A-yckABU

Plasmid

#112789

Purpose

Upstream homology arm, Pair of HAs spans yckA and yckB, which share homology with ABC transporters in B. subtilis

Depositor

Chris French

Publication

Wicke et al bioRxiv 185108

Insert

yckABU

Expression

Bacterial

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pTU1-C-cat

Plasmid

#112791

Purpose

Chloramphenicol resistance cassette (expressing chloramphenicol acetyltransferase)

Depositor

Chris French

Publication

Wicke et al bioRxiv 185108

Insert

cat

Expression

Bacterial

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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