We narrowed to 41,906 results for: tro

1. pEBTetBl-CLIP-Cep128

   Plasmid

   #136872

   Purpose

   Mammalian expression of the centrosomal protein Cep128 N-terminally fused to CLIP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   CLIP-Cep128 (CEP128 Synthetic, Human)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pS6-hα2M-H6

   Plasmid

   #128495

   Purpose

   Expresses human α2M (from G27 to S1468) in mammalian cells. With C-t H6x tag.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

   Insert

   A2M (G27-S1468) (A2M Human)
   

   Use

   Tags

   His tag (6x)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-N1/mCherry-DMDEx23

   Plasmid

   #211367

   Purpose

   Expresses a DMD exon 23 skipping reporter in mammalian cells (mCherry-DMDEx23)

   Depositor

   Ulrich Lächelt

   Article

   Lessl et al Bioconjug Chem. 2023 Dec 20;34(12):2263-

   Insert

   mCherry-DMDEx23
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   mCherry interrupted by mdx dystrophin exon 23 bet…

   Promoter

   CMV

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV Syn hBE Rh Guanylyl Cyclase1 2A tDimer

   Plasmid

   #66779

   Purpose

   The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling

   Depositor

   Thomas Oertner

   Article

   Scheib et al Sci Signal. 2015 Aug 11;8(389):rs8. doi:

   Inserts

   hbeRhGC
   red fluorescent protein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human Synapsin

   Available since

   Oct. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWPT-/mEGFP-1T-IRES-mCherry

   Plasmid

   #190190

   Purpose

   Lentiviral expression vector with altered Kozak sequence to direct EGFP expression. mCherry expression is regulated by an IRES.

   Depositor

   Alessandro Quattrone

   Article

   Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

   Inserts

   mEGFP
   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   changed EGFP Kozak sequence inserting a C>T mo…

   Promoter

   EIF1-short

   Available since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMYs-MFN2-IRES-mitoRFP

   Plasmid

   #121997

   Purpose

   Expression of MFN2 and mitoRFP in mammalial cells

   Depositor

   Qing Deng

   Article

   Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

   Insert

   MFN2-IRES-mitoRFP (MFN2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   silent Q386Q Q387Q V388V Y389Y C390C E391E E392E

   Promoter

   Available since

   Sept. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. CIBN-rTetR

   Plasmid

   #183919

   Purpose

   Optogenetic CIBN localizer domain coupled to reverse Tet repressor; binds tetO sites upon doxycycline addition; CIBN is bound by PHR upon blue light exposure

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   CIBN-rTetR (CIB1 Synthetic, Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEBTet-SNAP-Cep78

   Plasmid

   #136827

   Purpose

   Mammalian expression of the centrosomal protein Cep78 N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-Cep78 (CEP78 Synthetic, Human)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDisplay-GRAB_NPYmut

   Plasmid

   #208678

   Purpose

   Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) control sensor GRAB_NPYmut in mammalian cells

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based neuropeptide Y (NPY) control sensor GRAB_NPYmut
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBabe-TetCMV-puro-mVenus-PA-Rac1

    Plasmid

    #22008

    Purpose

    Mammalian expression of photoactivatable Rac1

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1 (RAC1 Human, Avena sativa (oat))
    

    Use

    Retroviral

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    LOV(Leu404-Leu546), Rac1 starts at I4 and contain…

    Promoter

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pEBTet-SNAP-Cep128

    Plasmid

    #136823

    Purpose

    Mammalian expression of the centrosomal protein Cep128 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Cep128 (CEP128 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. gfap-EGFP donor

    Plasmid

    #65564

    Purpose

    Intron targeting-mediated EGFP knockin at the zebrafish gfap locus

    Depositor

    Jiu-lin Du

    Article

    Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

    Insert

    gfap-EGFP (gfap Zebrafish)
    

    Use

    Zebrafish injection

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBactin-Nedd1-mNeonGreen

    Plasmid

    #196864

    Purpose

    Expression of neural precursor cell expressed developmentally down-regulated 1 (Need1) fused to mNeonGreen

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Nedd1-mNeonGreen (Nedd1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-RIαΔ(D/D+linker)-EGFP

    Plasmid

    #181843

    Purpose

    GFP-tagged PKA RIα subunit lacking the docking/dimerization (D/D) and linker regions; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    RIα-(D/D+linker)EGFP (PRKAR1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of residues 12-112 in PKA RIα.

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSCV-Flag-Zfp423-deltaSBD

    Plasmid

    #35971

    Depositor

    Bruce Spiegelman

    Article

    Gupta et al Nature. 2010 Mar 25;464(7288):619-23. do

    Insert

    Zinc Finger Protein 423 (Zfp423 Mouse)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    lacks zinc fingers 14-19 (aa 640 -838) (delta SMA…

    Promoter

    Available since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pEF5/FRT/DEST-3xHA/mGli2 Pc-gA

    Plasmid

    #51255

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 216,220,224,230,248 mutated to Ala

    Promoter

    Available since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pBabe-TetCMV-puro-mVenus-PA-Rac1-T17N

    Plasmid

    #22018

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mVenus-PA-Rac1-T17N (RAC1 Human, Avena sativa (oat))
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Rac1 starts at I4 and contains mutation T17N.

    Promoter

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pEBTet-SNAP-Cep76

    Plasmid

    #136813

    Purpose

    Mammalian expression of the centrosomal protein Cep76 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Cep76 (CEP76 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5-FRT-T0-Flag-HROB

    Plasmid

    #135298

    Purpose

    Expresses Flag-tagged HROB in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Hustedt et al Genes Dev. 2019 Oct 1;33(19-20):1397-141

    Insert

    HROB (HROB Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pEF5/FRT/DEST-3xHA/mGli2 Pc-gA/P1-6A

    Plasmid

    #51257

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248,789,805,817,848,923,956 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 216,220,224,230,248,789,805,817,848,9…

    Promoter

    EF1a

    Available since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only