We narrowed to 5,046 results for: cre encoding plasmid

1. pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA

   Plasmid

   #20299

   Purpose

   Cre-activated AAV expression of mCherry

   Depositor

   Karl Deisseroth

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-DIO-CAG-hM3Dq-P2A-dTomato

   Plasmid

   #202036

   Purpose

   AAV-mediated, cre-dependent expression of the pharmacogenetic actuator hM3Dq-2A-dTomato

   Depositor

   Peter Jonas, Yoav Ben-Simon

   Insert

   hM3Dq
   

   Use

   AAV

   Tags

   dTomato

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-DIO-CAG-hM4Di-P2A-dTomato

   Plasmid

   #202037

   Purpose

   AAV-mediated, cre-dependent expression of the pharmacogenetic actuator hM4Di-2A-dTomato

   Depositor

   Peter Jonas, Yoav Ben-Simon

   Insert

   hM4Di
   

   Use

   AAV

   Tags

   dTomato

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1a-double floxed-EYFP-WPRE-HGHpA

   Plasmid

   #20296

   Purpose

   Cre-activated AAV expression of EYFP

   Depositor

   Karl Deisseroth

   Insert

   EYFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. E. coli MEV20

   Bacterial Strain

   #197113

   Purpose

   E. coli BL21(DE3) derivative for diterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Bacterial Resistance

   None

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

6. E. coli MEV15

   Bacterial Strain

   #197112

   Purpose

   E. coli BL21(DE3) derivative for sesquiterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Bacterial Resistance

   None

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

7. pRDA_852

   Plasmid

   #185527

   Purpose

   Lentiviral vector that enables Cre-mediated expression of two sgRNAs. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

   Depositor

   Arlene Sharpe

   Inserts

   First sgRNA cassette
   Second sgRNA cassette
   

   Use

   CRISPR, Cre/Lox, and Lentiviral ; Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 and Mouse U6

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCALNL-tdTomato

   Plasmid

   #194205

   Purpose

   Cre/LoxP-dependent expression of a tdTomato. Expression of Cre excises a STOP codon before tdTomato, facilitating fluorescence expression of tdTomato.

   Depositor

   Angelique Bordey

   Insert

   tdTomato
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Human CRISPR Metabolic Gene Knockout Library

   Pooled Library

   #110066

   Purpose

   Encodes gRNAs for targeting metabolic genes in CRISPR screens.

   Depositor

   David Sabatini

   Article

   Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

   Use

   Lentiviral

   Available since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1-IL2sp-6His-Asprosin plasmid

    Plasmid

    #190683

    Purpose

    Expresses His-tagged asprosin in mammalian cells. An IL2 signal peptide at N-terminus allows secretion of asprosin from the transduced cells.

    Depositor

    Atul Chopra

    Article

    Mishra et al STAR Protoc. 2022 Dec 16;3(4):101762. do

    Insert

    IL2-6xHis-Asprosin
    

    Use

    Tags

    IL2 signal peptide, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT22iD36CriT(#388)

    Plasmid

    #184062

    Purpose

    cyclofen-inducible CRE activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Insert

    CRE-ERT2
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    Expression

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Dio3

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA

    Plasmid

    #20297

    Purpose

    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation

    Depositor

    Karl Deisseroth

    Has Service

    AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

    Insert

    channelrhodopsin-2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    humanized ChR2 gene with histidine 134 changed to…

    Promoter

    EF1alpha

    Available since

    June 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGY577

    Plasmid

    #166663

    Purpose

    Light-inducible Cre recombinase (LiCre) lentiviral expression plasmid for light-induced Cre/Lox recombination

    Depositor

    Gaël Yvert

    Article

    Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

    Insert

    LiCre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Cre: E340A, D341A

    Promoter

    CMV

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-nEF-Con/Fon DREADD Gi-mCherry

    Plasmid

    #177672

    Purpose

    Expresses DREADD hM4D (Gi) in Cre and Flp expressing cells

    Depositor

    Karl Deisseroth

    Has Service

    AAV8

    Insert

    DREADD Gi-mCherry
    

    Use

    AAV, Cre/Lox, and Mouse Targeting ; Intrsect

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    nEF

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA

    Plasmid

    #20298

    Purpose

    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

    Depositor

    Karl Deisseroth

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    channelrhodopsin-2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    humanized ChR2 gene with histidine 134 changed to…

    Promoter

    EF1alpha

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-nEF-Con/Foff DREADD Gi-mCherry

    Plasmid

    #177673

    Purpose

    Expresses DREADD hM4D (Gi) only in Cre expressing cells and not in Flp expressing cells

    Depositor

    Karl Deisseroth

    Has Service

    AAV8

    Insert

    DREADD Gi-mCherry
    

    Use

    AAV, Cre/Lox, and Mouse Targeting ; Intrsect

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    nEF

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-SEO-CaMKII-EGFP

    Plasmid

    #177018

    Purpose

    For expression of EGFP in a single-floxed, excisable open reading frame (cre-off), under control of the CaMKIIa promoter

    Depositor

    Peter Jonas

    Insert

    EGFP
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOTTC1761 - pAAV SYN1 DIO HA-hM3D(Gq)

    Plasmid

    #125147

    Purpose

    An adeno-associated viral vector expressing Cre-dependent HA-tagged inhibitory DREADD (hM3D(Gq)) from a synapsin promoter

    Depositor

    Christopher Richie

    Insert

    HA-tagged hM3D(Gq) (CHRM3 Human)
    

    Use

    AAV and Cre/Lox

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    SYN1

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOTTC1740 - pAAV SYN1 DIO HA-hM4D(Gi)

    Plasmid

    #125146

    Purpose

    An adeno-associated viral vector expressing Cre-dependent HA-tagged inhibitory DREADD (hM4D(Gi)) from a synapsin promoter

    Depositor

    Christopher Richie

    Insert

    HA-tagged hM4D(Gi) (CHRM4 Human)
    

    Use

    AAV and Cre/Lox

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    SYN1

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNK-TGCK

    Plasmid

    #45359

    Depositor

    Sean Wu

    Inserts

    tTA
    eGFP-Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Nkx2.5 cardiac enhancer and promoter fragme…

    Available since

    Aug. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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