We narrowed to 9,744 results for: try

1. pIIS18-BufAI-2

   Plasmid

   #128651

   Purpose

   Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

   Depositor

   Tohru Suzuki

   Article

   Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pIIS18-BufAI-1

   Plasmid

   #128650

   Purpose

   Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

   Depositor

   Tohru Suzuki

   Article

   Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CALCOCO1 L140A-mycDDK

   Plasmid

   #137767

   Purpose

   CALCOCO1 CLIR mutant

   Depositor

   Carol Mercer

   Article

   Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

   Insert

   CALCOCO1 L140A
   

   Tags

   mycDDK

   Expression

   Mammalian

   Mutation

   L140A

   Promoter

   CMV

   Available Since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CALCOCO1 R12H-mycDDK

   Plasmid

   #137770

   Purpose

   CALCOCO1 R12H mutant

   Depositor

   Carol Mercer

   Article

   Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

   Insert

   CALCOCO1 R12H
   

   Tags

   mycDDK

   Expression

   Mammalian

   Mutation

   R12H

   Promoter

   CMV

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDONR223 LC3B2 no-stop

   Plasmid

   #123207

   Purpose

   Gateway entry clone encoding human MAP1LC3B2 lacking stop codon, suitable for C-terminal tagging

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)
   

   Use

   Gateway entry vector / entry clone

   Mutation

   Stop codon removed

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRPa-c6MYCn

   Plasmid

   #69242

   Purpose

   Tet-inducible RRNA promoter driven plasmid for expression of N or C-terminally c-Myc tagged protein from a specific RRNA spacer locus in T. brucei.

   Depositor

   David Horn

   Article

   Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7

   Type

   Empty backbone

   Use

   Expression in trypanosoma brucei

   Tags

   cMyc

   Promoter

   RRNA promoter

   Available Since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRP-c6MYCn

   Plasmid

   #69243

   Purpose

   Tet-inducible RRNA promoter driven plasmid for inducible expression of N- or C-terminally c-Myc tagged protein from a random RRNA spacer locus in T. brucei.

   Depositor

   David Horn

   Article

   Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7

   Type

   Empty backbone

   Use

   Expression in trypanosoma brucei

   Tags

   cMyc

   Promoter

   RRNA promoter

   Available Since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRPa-iSL(MCS3+4)

   Plasmid

   #69246

   Purpose

   Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.

   Depositor

   David Horn

   Article

   Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7

   Type

   Empty backbone

   Use

   Expression in trypanosoma brucei

   Promoter

   RRNA promoter

   Available Since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPOTv7-hyg-AtAID:HA (p5339)

   Plasmid

   #239363

   Purpose

   pPOTv7 based plasmid template for addition of HA-epitope tagged Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using hygromycin

   Depositor

   Mark Carrington

   Article

   Alexandra et al bioRxiv 2025.06.17.660065

   Insert

   Arabidopsis Auxin Inducible Degradation domain
   

   Use

   Bacterial

   Tags

   1 x HA epitope

   Promoter

   none

   Available Since

   July 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)

    Plasmid

    #171129

    Purpose

    LwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)

    Depositor

    Benjamin Kleinstiver

    Article

    Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

    Insert

    U6 promoter entry plasmid for LwaCas13a crRNA cloning
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRSF-G1(7FTrp)RS

    Plasmid

    #177310

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al ACS Sens. 2022 Jan 10. doi: 10.1021/acss

    Insert

    tRNA synthetase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSMOX-mNG::LacI-V3

    Plasmid

    #210224

    Purpose

    Integrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Insert

    mNeongreen Lac repressor fusion protein
    

    Use

    Trypanosoma brucei expression

    Promoter

    Pol II readthrough transcription after integration

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTrex-Neo-tdTomato

    Plasmid

    #47975

    Purpose

    Trasgenically expresses tandem tdtomato fluorescent protein in T. cruzi

    Depositor

    Rick Tarleton

    Article

    Canavaci et al PLoS Negl Trop Dis. 2010 Jul 13;4(7):e74

    Insert

    tdTomato
    

    Use

    Trypanasoma cruzi expression

    Promoter

    T. cruzi rRNA

    Available Since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. MC177VSG221RNAi

    Plasmid

    #59730

    Purpose

    For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.

    Depositor

    Gloria Rudenko

    Article

    Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;10

    Insert

    VSG221 RNAi fragment
    

    Use

    RNAi

    Promoter

    opposing T7 promoters

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-EF1a-DIO-tdTomato-WPRE

    Plasmid

    #133786

    Purpose

    Cre-dependent tdTomato fluorescent reporter for cell labeling

    Depositor

    Michael Stryker

    Article

    Sun et al Proc Natl Acad Sci U S A. 2019 Oct 22;11

    Insert

    pAAV-EF1a-DIO-tdTomato-WPRE
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Available Since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRSF-G1(7AW)RS

    Plasmid

    #207516

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Aza-L-Tryptophan (7AW) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Abdelkader et al ACS Sens. 2023 Oct 27. doi: 10.1021/acss

    Insert

    tRNA synthetase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR223 LC3C WT

    Plasmid

    #123078

    Purpose

    Gateway entry clone encoding human MAP1LC3C wild-type

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)
    

    Use

    Gateway entry vector / entry clone

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMSP1D1R1

    Plasmid

    #97323

    Purpose

    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional R residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

    Depositor

    Michael Marty

    Article

    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

    Insert

    MSP1D1R1
    

    Tags

    6xHis - TEV

    Expression

    Bacterial

    Mutation

    Addition of R residue after T25

    Promoter

    T7

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMSP1D1G1

    Plasmid

    #97325

    Purpose

    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

    Depositor

    Michael Marty

    Article

    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

    Insert

    MSP1D1G1
    

    Tags

    6xHis - TEV

    Expression

    Bacterial

    Mutation

    Addition of G residue after T25

    Promoter

    T7

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR223 GABARAP WT

    Plasmid

    #123080

    Purpose

    Gateway entry clone encoding human GABARAP wild-type

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)
    

    Use

    Gateway entry vector / entry clone

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge