We narrowed to 8,386 results for: Dos

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Str-KDEL_SBP-EGFP-LAMP1-Y414A

Plasmid

#222319

Purpose

Synchronize the trafficking of LAMP1-Y414A from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAMP1-Y414A fused to SBP-EGFP (LAMP1 Human)

Expression

Mammalian

Mutation

Tyr414 to Ala

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-mCherry-LAMP1-Y414A

Plasmid

#222320

Purpose

Synchronize the trafficking of LAMP1-A17 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAMP1-Y414A fused to SBP-mCherry (LAMP1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-LAP

Plasmid

#222322

Purpose

Synchronize the trafficking of LAP from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAP fused to SBP-EGFP (ACP2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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AA498

Plasmid

#231119

Purpose

Fragmid fragment: (guide cassette) ABE activity-based selection CD274 positive control

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Insert

sgCD274 + ABE splice-targeting sgRNA

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AA505

Plasmid

#231120

Purpose

Fragmid fragment: (guide cassette) CBE activity-based selection CD274 positive control

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Insert

sgCD274 + CBE splice-targeting sgRNA

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pFB-Dual-MBP-NavAb (KAV/G94C/Q150C)

Plasmid

#226884

Purpose

Expresses a mutant of the Arcobacter butzleri voltage-gated sodium channel fused to an MBP tag in insect cells

Depositor

Ning Zheng

Article

Wisedchaisri et al Cell. 2019 Aug 8;178(4):993-1003.e12. do

Insert

NaVAb

Tags

MBP

Expression

Insect

Mutation

R4A, N49K, L109A, M116V

Promoter

Polyhedrin

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pJExpress401-KDAC8 H143A

Plasmid

#224342

Purpose

Expresses human KDAC8 (HDAC8) H143A in E. coli

Depositor

Terry Watt

Article

Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

Insert

KDAC8 H143A (HDAC8 Human)

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Histidine 143 mutated to alanine

Promoter

T5

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFastBacI-KDAC7 CD

Plasmid

#224345

Purpose

Expresses human KDAC7 (HDAC7) catalytic domain in insect cells

Depositor

Terry Watt

Article

Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

Insert

KDAC7 (HDAC7 Human)

Tags

TEV-cleavable His6

Expression

Insect

Mutation

Only includes residues 521-942 (catalytic domain).

Promoter

Polyhedrin

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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300_pETcon_SARS2_FLip

Plasmid

#222230

Purpose

yeast surface display of the SARS-CoV-2 FLip variant RBD

Depositor

Tyler Starr

Article

Taylor et al Virus Evol. 2024 Sep 2;10(1):veae067. do

Insert

SARS-CoV-2 FLip RBD (S SARS-CoV-2 virus, Budding Yeast)

Tags

HA and c-Myc

Expression

Yeast

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEXP(FLAG/HA-NSP1-K164-H165A)

Plasmid

#188782

Purpose

Expresses FLAG/HA-NSP1-K164-H165A in mammalian cells (SARS-CoV-2 mutant NSP1)

Depositor

Markus Hafner

Article

Anastasakis et al PLoS One. 2024 Jan 26;19(1):e0297262. do

Insert

FLAG/HA-NSP1-K164-H165A (SARS-CoV-2)

Use

Frt site to insert into hek293 t-rex genome

Tags

FLAG/HA

Expression

Mammalian

Mutation

Changed Lysine 164 to Alanine and Histidine 165 t…

Promoter

CMV Dox inducible

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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RT-5

Plasmid

#220442

Purpose

Construct used to generate the PUF3/PUP2/3HA tagging strains. (aka pBSII/PUF3 5’ flank/PUP2-DADA/3HA/URA3/PUF3 3’ flank)

Depositor

Marvin Wickens

Article

Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

Insert

PUF3 (puf-3 Nematode)

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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RT-4

Plasmid

#220443

Purpose

Construct used to generate the PUF3/PUP2/3HA tagging strains. Surrounding the PUP2/3HA/URA3 are large regions of homology to integrate at the 3’ end of the PUF3 gene.

Depositor

Marvin Wickens

Article

Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

Insert

PUF3 (PUF3 Budding Yeast)

Tags

3xHA

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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SCML2_3

Plasmid

#220878

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Article

Nady et al J Mol Biol. 2012 Nov 9;423(5):702-18. do

Insert

SCML2 (SCML2 Human)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

June 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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CMV DJ-1 C106A

Plasmid

#220914

Purpose

Expresses DJ-1 with the C106A mutation

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

DJ-1 (PARK7 Human)

Expression

Mammalian

Mutation

C106A

Promoter

CMV

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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AAV hSyn mRuby

Plasmid

#220912

Purpose

Neuronal specific expression of mRuby

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

mRuby

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pPI470

Plasmid

#207436

Purpose

Inducible extrachromosomal overexpression vector - mNeonGreen - C-terminal

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

C-terminal mNeonGreen

Use

Unspecified

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pPI450

Plasmid

#207433

Purpose

Inducible extrachromosomal overexpression vector - mNeonGreen - N-terminal

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

N-terminal mNeonGreen

Use

Unspecified

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pPI465

Plasmid

#207435

Purpose

REMI integrated Expression for mNeonGreen Fusions N-terminal

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

N-terminal mNeonGreen

Use

Unspecified

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pPI471

Plasmid

#207437

Purpose

REMI integrated Expression for mNeonGreen Fusions C-terminal

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

C-terminal mNeonGreen

Use

Unspecified

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-EFNB2 (D62Q)

Plasmid

#200976

Purpose

Mammalian expression plasmid for myc-tagged EFNB2 (D62Q specificity mutant)

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EFNB2 (EFNB2 Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

D62Q (increases specificity towards henipaviral G…

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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