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We narrowed to 11,576 results for: Vars;

Showing: 5781 - 5800 of 11576 results

  2. pEGB_3alpha2 OplacI:mini35S:RDF:Tnos (GB1534)

    Plasmid
    #160617
    Purpose
    TU for the regulated expression of the PhiC31 phage recombination directionality factor (RDF) gene driven by a synthetic promoter consisting of the LacI operator fused to the minimal 35S promoter.
    Depositor
    Diego Orzaez
    Article
    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379
    Insert
    RDF
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    mini35s
    Available Since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160617-plasmid-map-sequence-id-322019

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  3. pUPD2 PhiC31 RL (attR:P35S:TMtb:attL) (GB1506)

    Plasmid
    #160582
    Purpose
    35S promoter inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attR and attL)
    Depositor
    Diego Orzaez
    Article
    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379
    Insert
    attR:P35S:TMtb:attL
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available Since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160582-plasmid-map-sequence-id-322083

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  4. pUPD 5'UTR:YFP:Tnos (GB1483)

    Plasmid
    #160557
    Purpose
    Part 3/3 for assembly of site-specific recombination based Registers. Composed of the 5'UTR sequence of 35S promoter, coding sequence of YFP and NOS terminator (5'UTR:YFP:TNos)
    Depositor
    Diego Orzaez
    Article
    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379
    Insert
    5'UTR:YFP:Tnos
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available Since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160557-plasmid-map-sequence-id-324725

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  6. pUPD2 Assembly Register 3/3 Part - 5'UTR:Luc:Tnos (GB1500)

    Plasmid
    #160581
    Purpose
    Part 3/3 for assembly of site-specific recombination based Registers. Composed of the 5'UTR sequence of 35S promoter, coding sequence of Luc and NOS terminator
    Depositor
    Diego Orzaez
    Article
    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379
    Insert
    Luc
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available Since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160581-plasmid-map-sequence-id-322088

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  7. pEGB3 alpha2 OplexA:mini35S:PhiC31int:Tnos (GB1529)

    Plasmid
    #160614
    Purpose
    TU for the regulated expression of the PhiC31 phage integrase gene driven by a synthetic promoter consisting of the LexA operator fused to the minimal 35S promoter.
    Depositor
    Diego Orzaez
    Article
    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379
    Insert
    PhiC31
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    mini35s
    Available Since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160614-plasmid-map-sequence-id-322024

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  8. pEGB_3alpha1 OplacI:mini35S:phiC31:Tnos (GB1530)

    Plasmid
    #160615
    Purpose
    TU for the regulated expression of the PhiC31 phage integrase gene driven by a synthetic promoter consisting of the LacI operator fused to the minimal 35S promoter.
    Depositor
    Diego Orzaez
    Article
    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379
    Insert
    PhiC31
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    mini35s
    Available Since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160615-plasmid-map-sequence-id-322022

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  13. B-HypaSpCas9

    Plasmid
    #126763
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-HypaSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HypaSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-HypaSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N692A; M694A; Q695A; H698A; amino acids 1005-1013…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126763-plasmid-map-sequence-id-410968

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  14. pET-FLAG-B-eSpCas9

    Plasmid
    #126772
    Purpose
    Expression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-eSpCas9
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    K848A; K1003A; R1060A; amino acids 1005-1013 repl…
    Promoter
    T7
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126772-plasmid-map-sequence-id-410600

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  15. pX330-Flag-SpCas9-HF1 (without sgRNA; with silent mutations)

    Plasmid
    #126755
    Purpose
    Expression plasmid for human codon-optimized increased fidelity SpCas9-HF1 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    SpCas9-HF1
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126755-plasmid-map-sequence-id-410971

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Showing: 5781 - 5800 of 11576 results