We narrowed to 7,602 results for: Pet2;

1. pET28a-anticalin (S87amb)-His

   Plasmid

   #168044

   Purpose

   Expresses anticalin targeting CTLA-4 with the codon encoding S87 changed to amber codon (TAG).

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Tags

   6x Histag

   Expression

   Bacterial

   Mutation

   Ser 87 was mutated to amber codon (TAG)

   Promoter

   T7 promoter

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-anticalin (N116amb)-His

   Plasmid

   #168045

   Purpose

   Expresses anticalin targeting CTLA-4 with the codon encoding N116 changed to amber codon (TAG).

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Tags

   6x Histag

   Expression

   Bacterial

   Mutation

   Asn 116 was mutated to amber codon (TAG)

   Promoter

   T7 promoter

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-anticalin (N21amb)-His

   Plasmid

   #168041

   Purpose

   Expresses anticalin targeting CTLA-4 with the codon encoding N21 changed to amber codon (TAG).

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Tags

   6x Histag

   Expression

   Bacterial

   Mutation

   Asn 21 was mutated to amber codon (TAG)

   Promoter

   T7 promoter

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-anticalin (I55amb)-His

   Plasmid

   #168042

   Purpose

   Expresses anticalin targeting CTLA-4 with the codon encoding I55 changed to amber codon (TAG).

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Tags

   6x Histag

   Expression

   Bacterial

   Mutation

   Ile 55 was mutated to amber codon (TAG)

   Promoter

   T7 promoter

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Pet22b-mEGFP-YAP-C-IDR

   Plasmid

   #166441

   Purpose

   Bacterial expression of 6x His tagged mEGFP-YAP1-C-IDR fusion protein

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   mEGFP, YAP-C-IDR(266-504) (YAP1 Human)
   

   Expression

   Bacterial

   Available Since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. BPV00914 pET21GG2 H6-TF-BECN (1-450) [VA-MA-LA]

   Plasmid

   #136688

   Purpose

   Expresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor). Three mutations are added to the CC and ECD/BARA interface.

   Depositor

   Colin Garvie

   Article

   Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

   Insert

   Beclin-1 (BECN1 Human)
   

   Tags

   Trigger Factor

   Expression

   Bacterial

   Mutation

   DNA optimized for E. coli expression w/ three mut…

   Promoter

   T7

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET24a-VHH-tev-2xTS

   Plasmid

   #117750

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP, tev cleavage site and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37

   Insert

   anti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
   

   Tags

   BAP, HA, His6, T7, and Tyrosine sulfation (TS) se…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET29b-clbH-C-A2-PCP S1524A

   Plasmid

   #114158

   Purpose

   Expresses ClbH-C-A2-PCP S1524A

   Depositor

   Emily Balskus

   Article

   Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

   Insert

   clbH
   

   Tags

   S-Tag, Thrombin site

   Expression

   Bacterial

   Mutation

   Only contains the C-A2-PCP domains of ClbH with S…

   Available Since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a_6HT-TBP6.7(delta C-term)

   Plasmid

   #112731

   Purpose

   This mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(delta C-term)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   delta C-term

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET22b-ecDHFR-dCys-T46C

    Plasmid

    #110543

    Purpose

    Bacterial expression of E.coli DHFR C85A/C152S/T46C mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

    Insert

    ecDHFR
    

    Expression

    Bacterial

    Mutation

    T46 mutated to C46, C85 mutated to A85, C152 muta…

    Promoter

    T7

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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