We narrowed to 150,751 results for: ins

1. pMIIS793

   Plasmid

   #238206

   Purpose

   Expresses TaTasR with an N-terminal Twin-Strep-SUMO tag. KanR

   Depositor

   Feng Zhang

   Article

   Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

   Insert

   TaTasR
   

   Tags

   TwinStrepII-bdSUMO

   Expression

   Bacterial

   Available Since

   May 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIRE4-Azi

   Plasmid

   #105829

   Purpose

   Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells

   Depositor

   Irene Coin

   Article

   Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.

   Inserts

   Humanized EAziRS
   4 copies of the U6-BstYam tRNA cassette
   

   Expression

   Mammalian

   Mutation

   (Y37L, D182S, F183M, L186A, D265R)TyrRS

   Promoter

   PGK and U6

   Available Since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBABE puro human IRS-1

   Plasmid

   #11025

   Depositor

   Ronald Kahn

   Article

   Bruning et al Mol Cell Biol. 1997 Mar . 17(3):1513-21.

   Insert

   IRS-1 (IRS1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. BPK764

   Plasmid

   #65767

   Purpose

   Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Bacterial

   Promoter

   T7 (x2)

   Available Since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-hLC3B-pcDNA3.1

   Plasmid

   #40827

   Depositor

   David Rubinsztein

   Article

   Jahreiss et al Traffic. 2008 Apr;9(4):574-87. Epub 2008

   Insert

   LC3B (MAP1LC3B Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV human IRS-1 CAAX

   Plasmid

   #11238

   Depositor

   Ronald Kahn

   Article

   Kriauciunas et al Mol Cell Biol. 2000 Sep . 20(18):6849-59

   Insert

   IRS-1 (IRS1 Human)
   

   Tags

   CAAX

   Expression

   Mammalian

   Mutation

   C-terminal prenylation motif makes IRS-1 localize…

   Available Since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3.1 3xFlag IFIT1

   Plasmid

   #53554

   Purpose

   mammalian expression of IFIT1

   Depositor

   Kathleen Collins

   Article

   Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

   Insert

   IFIT1 (IFIT1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. IK349

   Plasmid

   #233655

   Purpose

   Dox-inducible red light-inducible Flp-C; Flp-397C-PhyA

   Depositor

   Wilson Wong

   Article

   Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

   Insert

   Flp-397C-PhyA
   

   Expression

   Mammalian

   Mutation

   None

   Promoter

   TRE3G

   Available Since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. IK331

   Plasmid

   #233653

   Purpose

   Dox-inducible blue light-inducible Cre-N; Cre-N229-pmag

   Depositor

   Wilson Wong

   Article

   Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

   Insert

   Cre-N229-pmag
   

   Expression

   Mammalian

   Mutation

   None

   Available Since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. IK332

    Plasmid

    #233654

    Purpose

    Dox-inducible blue light-inducible Cre-C; Cre-230C-nmag

    Depositor

    Wilson Wong

    Article

    Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

    Insert

    Cre-230C-nmag
    

    Expression

    Mammalian

    Mutation

    None

    Available Since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGPTVII-Bar-U-MatryoshCaMP6s

    Plasmid

    #100024

    Purpose

    Plant expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    MatryoshCaMP6s
    

    Expression

    Plant

    Promoter

    AtUBQ10

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. dCas9-10xSunTag-P2A-tagBFP

    Plasmid

    #174141

    Purpose

    Expresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid.

    Depositor

    Albert Jeltsch

    Article

    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

    Insert

    dCas9, 10xSunTag, tagBFP
    

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CAG-HcKCR1-EYFP

    Plasmid

    #182021

    Purpose

    Express light-gated potassium channel HcKCR1-EYFP in mammalian cells

    Depositor

    Mingshan Xue

    Article

    Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

    Insert

    HcKCR1
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pR I-Ab alpha BirA

    Plasmid

    #113638

    Purpose

    Insect cell expression vector for I-Ab alpha chain fused to BirA biotinylation site

    Depositor

    James Moon

    Article

    Moon et al Methods Mol Biol. 2018;1799:165-181. doi

    Insert

    I-Ab alpha BirA (H2-Ab1 Mouse)
    

    Tags

    BirA biotinylation signal site and fused to Fos-J…

    Expression

    Insect

    Mutation

    transmembrane and cytoplasmic domains truncated

    Promoter

    Metallothionein

    Available Since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFastBac HT JS-Munc18c

    Plasmid

    #135555

    Purpose

    Express mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    Munc18c
    

    Tags

    6xHis tag and TEV site

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-ChroME2f-mRuby2-ST

    Plasmid

    #170160

    Purpose

    Cation channelrhodopsin ChroME2f fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChroME2f-mRuby2-ST
    

    Expression

    Mammalian

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. 2Bc-T MBP_BoMoC(ed)_W403A_F753A_6xH

    Plasmid

    #185713

    Purpose

    Recombinant B. mori truncated R2 RT expression

    Depositor

    Kathleen Collins

    Article

    Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

    Insert

    B. mori truncated R2 RT
    

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Changed W403 and F753 to A

    Promoter

    T7

    Available Since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRII_TOPO_hNEAT1

    Plasmid

    #61518

    Purpose

    human NEAT1_v1 short isoform 3.7kb insert in general cloning vector

    Depositor

    Archa Fox

    Article

    Clemson et al Mol Cell. 2009 Mar 27;33(6):717-26. doi:

    Insert

    NEAT1 (NEAT1 Human)
    

    Use

    General cloning vector

    Mutation

    *see comment below

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. CMV500 A-FOS

    Plasmid

    #33353

    Depositor

    Charles Vinson

    Article

    Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

    Insert

    FOS (Fos Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Dominant Negative of c-Jun; heterodimerizes and i…

    Promoter

    CMV

    Available Since

    Dec. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDB180

    Plasmid

    #80677

    Purpose

    for secretion of N-terminal OsmY, deca-histidine, SUMO tagged protein

    Depositor

    David Baker

    Article

    Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

    Type

    Empty backbone

    Tags

    OsmY-10His-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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