We narrowed to 150,751 results for: ins

1. pCDNA3-myc3-ROC1

   Plasmid

   #20717

   Depositor

   Yue Xiong

   Article

   He et al Genes Dev. 2006 Nov 1. 20(21):2949-54.

   Insert

   RING finger protein 1 (ROC1) (RBX1 Human)
   

   Tags

   3xMyc

   Expression

   Mammalian

   Available Since

   March 31, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSF-G1(7FTrp)RS

   Plasmid

   #177310

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Qianzhu et al ACS Sens. 2022 Jan 10. doi: 10.1021/acss

   Insert

   tRNA synthetase
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACUH-GFP1-10 mitochondrial matrix

   Plasmid

   #172063

   Purpose

   UAS vector to express GFP1-10 in the mitochondrial matrix

   Depositor

   Daichi Kamiyama

   Article

   Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   GFP1-10 mitochondrial matrix
   

   Expression

   Insect

   Available Since

   Oct. 20, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pSEM318 - sgRNA mosTI unc-119 single-copy

   Plasmid

   #159822

   Purpose

   Single copy or array insertion by MosTI using split unc-119 selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   sgRNA mosTI unc-119 single-copy
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLL7.0: tgRFPt-SSPB WT

   Plasmid

   #60415

   Purpose

   Mammalian Expression of tgRFPt-Nano

   Depositor

   Brian Kuhlman

   Article

   Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

   Insert

   tgRFPt-SSPB
   

   Use

   Cre/Lox and Lentiviral

   Tags

   TagRFPt

   Expression

   Mammalian

   Mutation

   Y11K and A15E

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-P4:gs:nls:CIBN

   Plasmid

   #135986

   Purpose

   Expresses the P4 coiled coil fused to the CIBN blue light-inducible domain in mammalian cells

   Depositor

   Roman Jerala

   Article

   Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

   Insert

   P4 coiled-coil fused to the CIBN domain
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pECIA2

   Plasmid

   #47032

   Purpose

   This is a Gateway destination vector for secreted expression in Drosophila cell culture by induction via CuSO4. Inserts will be C-terminally tagged with Fc, V5 and hexahistidine tags.

   Depositor

   Chris Garcia

   Article

   Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.

   Type

   Empty backbone

   Use

   Secreted expression in drosophila culture

   Tags

   Fc(human IgG1), HRV 3C Protease Cleavage site, He…

   Expression

   Insect

   Promoter

   Metallothionein (Copper-Inducible)

   Available Since

   Aug. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19 PKC delta

   Plasmid

   #8427

   Depositor

   Frederic Mushinski

   Article

   Mischak et al Biochemistry 1991 Aug 13;30(32):7925-31.

   Insert

   Mouse Protein Kinase C delta (Prkcd Mouse)
   

   Expression

   Bacterial

   Available Since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRVL-5

   Plasmid

   #104583

   Purpose

   Contains human IgG1 CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functions

   Depositor

   Daniel Christ

   Article

   Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

   Insert

   Human IgG1 CH1/CH2/CH3 (IGHG1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a Gx-NL

    Plasmid

    #169105

    Purpose

    Expresses protein G fused to NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - NanoLuc
    

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available Since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSEM319 - array co-injection fragment mosTI unc-119

    Plasmid

    #159823

    Purpose

    Single copy or array insertion by MosTI using split unc-119 selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    array co-injection fragment mosTI unc-119
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLL7.0: Venus-iLID-Mito (From ActA)

    Plasmid

    #60413

    Purpose

    Mammalian Expression of Venus-iLID-Mito

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    Venus-iLID-Mito
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CAG-UnaG-P2A-FusionRed

    Plasmid

    #191109

    Purpose

    Mammalian cell line expression plasmids for production of UnaG and FusionRed proteins

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    UnaG
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    chicken β-actin promoter

    Available Since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a Gx-mNG-NL

    Plasmid

    #169106

    Purpose

    Expresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - mNeonGreen - NanoLuc
    

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRVL-3

    Plasmid

    #104581

    Purpose

    Contains mouse IgG2c CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions

    Depositor

    Daniel Christ

    Article

    Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

    Insert

    Mouse IgG2c CH1/CH2/CH3 (mutated, no effector functions)
    

    Expression

    Mammalian

    Mutation

    L234A/L235E/G237A and E318A/K320A/K322A

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLL7.0: Venus-iLID-CAAX (from KRas4B)

    Plasmid

    #60411

    Purpose

    Mammalian Expression of Venus-iLID-CAAX

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    Venus-iLID-CAAX
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR_sfGFP-TurboID

    Plasmid

    #240226

    Purpose

    Gateway entry clone with TurboID tagged with superfolder GFP (contains stop codon)

    Depositor

    Justin Bosch, Norbert Perrimon

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Insert

    sfGFP-TurboID
    

    Use

    Gateway shuttle vector

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX Rin1-SH2

    Plasmid

    #46474

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Rin1 (RIN1 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 54-204

    Promoter

    Tac

    Available Since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)-MC38-TCRA-33-MC_hammer

    Plasmid

    #138989

    Purpose

    IVT template for the alpha subunit of the mouse TCR #33 that is reactive against MC38

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    TCRA (Tcra Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLL7.0: tgRFPt-SSPB R73Q

    Plasmid

    #60416

    Purpose

    Mammalian Expression of tgRFPt-Micro

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    tgRFPt-SSPB R73Q
    

    Use

    Cre/Lox and Lentiviral

    Tags

    TagRFPt

    Expression

    Mammalian

    Mutation

    R73Q, Y11K and A15E

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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