We narrowed to 12,091 results for: pcdna3.1

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HA-KIF5A

Plasmid

#166958

Purpose

Expresses HA-tagged KIF5A protein in pcDNA3.1

Depositor

Rosalind Segal

Publication

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KIF5A (Kif5a Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MTS-KD-c-Src-FLAG

Plasmid

#44653

Depositor

Yoshimi Homma

Publication

Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)

Tags

FLAG tag and mitochondria-targeting sequence (MTS…

Expression

Mammalian

Mutation

changed lysine 298 to methionine

Promoter

CMV promoter

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3-MTS-KD-c-Src-FLAG

Plasmid

#44653

Depositor

Yoshimi Homma

Publication

Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)

Tags

FLAG tag and mitochondria-targeting sequence (MTS…

Expression

Mammalian

Mutation

changed lysine 298 to methionine

Promoter

CMV promoter

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Gal4-HA-HIF1α(530-652)

Plasmid

#24887

Depositor

William Kaelin

Publication

Ivan et al Science. 2001 Apr 20. 292(5516):464-8.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hypoxia inducible factor1alpha (HIF1A Human)

Tags

Gal4dbd and HA-tag

Expression

Mammalian

Mutation

partial HIF1alpha cDNA (encoding aa 530-652)fused…

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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His-Precission-NEDD4L (381-975)_pcDNA3.1(+)

Plasmid

#187725

Purpose

Express his-tagged NEDD4L W2-W3-W4-HECT (381) in mammalian cells

Depositor

Sandra Gabelli

Publication

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 381-975 (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Mammalian

Mutation

deletion of residues 1-380

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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His-Precission-NEDD4L (492-975)_pcDNA3.1(+)

Plasmid

#187726

Purpose

Express his-tagged NEDD4L W3-W4-HECT in mammalian cells

Depositor

Sandra Gabelli

Publication

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 492-975 (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Mammalian

Mutation

deletion of residues 1-491

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot4_275-575-del377-402_AG

Plasmid

#148753

Purpose

Mammalian Expression of HsNot4_275-575-del377-402

Depositor

Elisa Izaurralde

Publication

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-del377-402 (CNOT4 Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot4_275-575-del377-424_AG

Plasmid

#148754

Purpose

Mammalian Expression of HsNot4_275-575-del377-424

Depositor

Elisa Izaurralde

Publication

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-del377-424 (CNOT4 Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot4_275-575-del400-428_AG

Plasmid

#148755

Purpose

Mammalian Expression of HsNot4_275-575-del400-428

Depositor

Elisa Izaurralde

Publication

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-del400-428 (CNOT4 Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg5-del133-155-G120E_T

Plasmid

#147554

Purpose

Mammalian Expression of HsSmg5-del133-155-G120E

Depositor

Elisa Izaurralde

Publication

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-del133-155-G120E (SMG5 Human)

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HshnRNPH1_185-449-del291-361_Z

Plasmid

#148123

Purpose

Mammalian Expression of HshnRNPH1_185-449-del291-361

Depositor

Elisa Izaurralde

Insert

HshnRNPH1_185-449-del291-361 (HNRNPH1 Human)

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Myr-DnaJB1(H33Q)-PKAcat-mCherry

Plasmid

#181855

Purpose

mCherry-tagged DnaJB1-PKA catalytic subunit fusion N-terminal myristoylation site and H33Q DnaJB1 mutation to block Hsp70 binding; for mammalian expression.

Depositor

Jin Zhang

Publication

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

Myr-DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Histidine 33 in DnaJB1 changed to glutamine.

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-tdMCP2-GyG-(GGS)X6-HaloTag C261

Plasmid

#171434

Purpose

Expression of tdMCP2-GyG-(GGS)X6-HaloTag C261 in mammalian cells

Depositor

Yujie Sun

Publication

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

tdMCP2-GyG-(GGS)X6-HaloTag C261

Expression

Mammalian

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG F370G

Plasmid

#169960

Purpose

Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)

Depositor

Hao Wu

Publication

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG and Ub

Expression

Mammalian

Mutation

F370G

Promoter

CMV

Available since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8-FLAG R394E + S297A

Plasmid

#169955

Purpose

Mammalian expression of CARD8-FLAG with a site III mutation (R394E) with an additional autoprocessing mutation (S297A)

Depositor

Hao Wu

Publication

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S297A, R394E

Promoter

CMV

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8-FLAG F405G + S297A

Plasmid

#169957

Purpose

Mammalian expression of CARD8-FLAG with a site III mutation (F405G) with an additional autoprocessing mutation (S297A)

Depositor

Hao Wu

Publication

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S297A, F405G

Promoter

CMV

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG L368G

Plasmid

#169959

Purpose

Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)

Depositor

Hao Wu

Publication

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG and Ub

Expression

Mammalian

Mutation

L368G

Promoter

CMV

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG R394E

Plasmid

#169961

Purpose

Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)

Depositor

Hao Wu

Publication

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG and Ub

Expression

Mammalian

Mutation

R394E

Promoter

CMV

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG F405G

Plasmid

#170119

Purpose

Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed).

Depositor

Hao Wu

Publication

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG and Ub

Expression

Mammalian

Mutation

F405G

Promoter

CMV

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8-FLAG E274R + S297A

Plasmid

#169951

Purpose

Mammalian expression of CARD8-FLAG that cannot bind DPP9 due to a mutation at site I (E274R) with an additional autoprocessing mutation (S297A)

Depositor

Hao Wu

Publication

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

E274R, S297A

Promoter

CMV

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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