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API5-CRISPR

Plasmid

#157664

Purpose

Expresses Cas9 and sgRNA targeting API5

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Apoptosis inhibitor 5 (API5 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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815 pSG5L HA PTEN delta274-342

Plasmid

#10738

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Mutation

Deletion of amino acids 274-342.

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_SNORD27h_115mut

Plasmid

#73068

Purpose

expression clone for human mutated SNORD27 (C/D box snoRNA U27)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD27 (SNORD27 Human)

Tags

no tag

Expression

Mammalian

Mutation

expression cassette for SNORD27 with AS box from …

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
p6596 MSCV-IP-N-HA ADRB2

Plasmid

#34895

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

ADRB2 (ADRB2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only
mEmerald-MyosinIIC-N-18

Plasmid

#54195

Purpose

Localization: Cytoskeleton, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Ebrahim et al Curr Biol. 2013 Apr 22;23(8):731-6. doi:

Insert

MyosinIIC

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
824 pGEX2T PTEN delta274-342

Plasmid

#10739

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Tags

GST

Expression

Bacterial

Mutation

Deletion of amino acids 274-342.

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
U6_sgRNA_CAG_hSt1Cas9_CNRZ1066

Plasmid

#136651

Purpose

A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:CNRZ1066 chimera) and its U6-driven sgRNA.

Depositor

Yannick Doyon

Article

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD-9:CNRZ_1066 Chimera
sgRNA for St1Cas9

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS

Expression

Mammalian

Promoter

CAG and hU6

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pbIG-Xph20-ETWV

Plasmid

#133034

Purpose

Expresses Xph20-ETWV in bacteria

Depositor

Matthieu Sainlos

Article

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Insert

Xph20-ETWV

Tags

AviTag, His10, and TEV protease cleavage site

Expression

Bacterial

Mutation

S63K

Promoter

T7

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCSP1-mp53AS-6xMUT

Plasmid

#20904

Depositor

Stefano Piccolo

Article

Cordenonsi et al Science. 2007 Feb 9. 315(5813):840-3.

Insert

p53AS (Trp53 Mouse)

Expression

Mammalian

Mutation

point mutation (Ser/Thr-Ala) of the 6 N-terminal …

Available Since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
pFLAG-WTS-1

Plasmid

#66856

Purpose

Express FLAG-tagged C.elegans WTS-1 in mammalian cells

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

Insert

WTS-1 (wts-1 Nematode)

Tags

FLAG

Expression

Mammalian

Mutation

E153G and D871G mutations in WTS1 compared to Gen…

Promoter

CMV

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
p6615 MSCV-IP N-HAonly NIPA1

Plasmid

#35013

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

NIPA1 (RBCK1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Feb. 9, 2012

Availability

Academic Institutions and Nonprofits only
pLVX-CAG-Fhl2-HA-P2A-mitomeGFP

Plasmid

#219576

Purpose

Lentiviral construct expressing HA-tagged Fhl2 followed by a P2A ribosome skip sequence and then a mitochondria targeted mEGFP.

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

Fhl2 (Fhl2 Rat)

Use

Lentiviral

Tags

HA-P2A-mitomeGFP-

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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hTRAK1(S200A, S201A, S719A, S919A)

Plasmid

#225225

Purpose

Expresses MYC-tagged hTRAK1 (S200A, S201A, S719A, S919A)

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

trak1 (TRAK1 Human)

Tags

MYC

Expression

Mammalian

Mutation

S200A, S201A, S719A, S919A mutations

Promoter

CMV

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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Fhl2-HA-P2A-meGFP(omp25mts)

Plasmid

#225231

Purpose

Construct expressing HA-tagged Fhl2 followed by a P2A ribosome skip sequence and then a mitochondria targeted mEGFP

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

Fhl2 (Fhl2 Rat)

Tags

HA-P2A-mitomeGFP-

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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MYC-hTRAK1(S200A, S201A)

Plasmid

#219572

Purpose

Expresses MYC-tagged hTRAK1 (S200A, S201A)

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

trak1 (TRAK1 Human)

Tags

MYC

Expression

Mammalian

Mutation

Serine 200 changed to alanine, serine 201 changed…

Promoter

CMV

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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MYC-hTRAK1(S200A, S201A, S719A, T834A, S919A)

Plasmid

#219574

Purpose

Expresses MYC-tagged hTRAK1 (S200A, S201A, S719A, T834A, S919A)

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

trak1 (TRAK1 Human)

Tags

MYC

Expression

Mammalian

Mutation

S200A, S201A, S719A, T834A, S919A mutations

Promoter

CMV

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-Gpr45-S214P-3xFlag

Plasmid

#245987

Purpose

Express 3xFlag C-terminal tagged mouse GPR45-S214P mutant transiently via transfection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gpr45 (Gpr45 Mouse)

Tags

3xFlag

Expression

Mammalian

Mutation

S214P

Promoter

CMV

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pEF1-EGFP-coilin-FRT

Plasmid

#247448

Purpose

FLP/FRT based mammalian expression vector for EGFP-coilin, driven by EF1α promoter.

Depositor

Kazuhiro Maeshima

Article

Itoh et al Life Sci Alliance. 2021 Feb 3;4(4):e2020

Insert

coilin (COIL) (COIL Human)

Tags

EGFP

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pBOB-Adcy3-HA

Plasmid

#245988

Purpose

Express HA C-terminal tagged wild-type mouse ADCY3 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Adcy3 (Adcy3 Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pBOB-Adcy3-L278H-3xFlag

Plasmid

#245990

Purpose

Express 3xFlag C-terminal tagged mouse ADCY3-L278H mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Adcy3 (Adcy3 Mouse)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

L278H

Promoter

CMV

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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