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pDONR223_CSNK1A1_WT

Plasmid

#82106

Purpose

Gateway Donor vector containing CSNK1A1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CSNK1A1 (CSNK1A1 Human)

Use

Gateway entry vector

Mutation

Q231K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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AICSDP-4:TUBA1B-mEGFP

Plasmid

#87421

Purpose

Homology arms and linker-mEGFP sequence for N-terminus (second exon) tagging of human TUBA1B

Depositor

Allen Institute for Cell Science

Article

Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

Insert

TUBA1B Homology Arms with mEGFP-linker (TUBA1B Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

March 15, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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sgTP53_3

Plasmid

#78164

Purpose

sgTP53

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

TP53 (TP53 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pD649-HAsp-CTLA4-COMP5AP-AviTag-9xHis

Plasmid

#157074

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CTLA4 (CTLA4 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pIG-719 CTLA4-GFP R70W

Plasmid

#186124

Purpose

CTLA4 gene knockin with mutation

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CTLA4 (CTLA4 Human)

Use

CRISPR

Mutation

R70W

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGEMT-TPE2A-Mef2c-Tdtomato-Gata4-Tbx5

Plasmid

#111818

Purpose

Quadcistronic construct: Co-express four genes by three 2A peptides -- T2A, P2A and E2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Inserts

Mef2c (Mef2c Mouse)
Tdtomato
Gata4 (Gata4 Mouse)
Tbx5

Use

Cloning intermediate

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-T790M

Plasmid

#116310

Purpose

Lentiviral expression of EGFR T790M

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

T790M

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLX304-ARNT

Plasmid

#203578

Purpose

Expresses wild-type ARNT in mammalian cells.

Depositor

Abhishek Chakraborty, William Kaelin

Article

Chakraborty et al Science. 2019 Mar 15;363(6432):1217-1222

Insert

ARNT (ARNT Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-CIB1-mCerulean-Rab11

Plasmid

#140574

Purpose

For use in light-induced protein inactivation through the interaction with CRY2

Depositor

Heidi Hehnly

Article

Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

Inserts

CIB1 (CIB1 Mustard Weed)
Rab11A (RAB11A Human)

Tags

mCerulean

Expression

Mammalian

Promoter

CMV IE94

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAP2K1_WT

Plasmid

#81920

Purpose

Gateway Donor vector containing MAP2K1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAP2K1 (MAP2K1 Human)

Use

Gateway entry vector

Mutation

I310N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAP2K1_p.P124S

Plasmid

#81703

Purpose

Gateway Donor vector containing MAP2K1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAP2K1 (MAP2K1 Human)

Use

Gateway entry vector

Mutation

P124S; I310N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCDH-MSCV-hCD38-EF1α-GFP+Puro

Plasmid

#134936

Purpose

expression human CD38 in mammalian cells

Depositor

Jianjun Zhao

Article

Hu et al Cancer Res. 2020 Mar 19. pii: 0008-5472.

Insert

CD38 (CD38 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_DKK1_WT

Plasmid

#82250

Purpose

Gateway Donor vector containing DKK1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

DKK1 (DKK1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs-HRAS(G12V)-IRES-SNAP-p53DD

Plasmid

#233200

Purpose

Retroviral expression of HRAS(G12V) and SNAP-p53DD

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

HRAS(G12V)-IRES-SNAP-p53DD (Trp53, HRAS Mouse, Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-SNAP-p53DD-IRES-HRAS(G12V)

Plasmid

#233198

Purpose

Retroviral expression of SNAP-p53DD and HRAS(G12V)

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

SNAP-p53DD-IRES-HRAS(G12V) (HRAS, Trp53 Mouse, Human)

Use

Retroviral

Expression

Mammalian

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pTAHR TH-p2a-TD:Tomato (floxed selection Puro)

Plasmid

#135814

Purpose

Fluorescent reporter for knock-in of TdTomato into the TH locus

Depositor

Tim Ahfeldt, Lee Rubin

Article

Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

Insert

TH-p2a-TD:Tomato (red fluorophore protein) construct as a reporter for TH expression. (TH Human)

Tags

p2a-TdTomato

Expression

Mammalian

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-EDA2R-COMP5AP-AviTag-9xHis

Plasmid

#157096

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

EDA2R (EDA2R Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_PML_RARA

Plasmid

#205897

Purpose

Express mEGFP-tagged fusion protein, PML_RARA from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

PML_RARA (PML, RARA Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-BCL2

Plasmid

#116717

Purpose

Lentiviral expression of BCL2

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BCL2 (BCL2 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_ERBB2_PPP1R1B

Plasmid

#205813

Purpose

Express mEGFP-tagged fusion protein, ERBB2_PPP1R1B from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

ERBB2_PPP1R1B (ERBB2, PPP1R1B Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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