Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 63,044 results for: cloning

Clear keywords
Showing: 5841 - 5860 of 63044 results

  9. pDONR P4-P1R-mKate2

    Plasmid
    #48344
    Purpose
    Contributes the coding sequence for the mKate2 fluorescent protein as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.
    Depositor
    Anna Planas, Tomas Santalucia
    Publication
    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:
    Insert
    mKate2
    Use
    Gateway Cloning
    Mutation
    It contains a Kozak sequence but no stop codon.
    Promoter
    none
    Available since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    48344-plasmid-map-sequence-id-70388

    Enlarge

  11. pOT2-poly-cis

    Plasmid
    #141177
    Purpose
    To clone any gene or STTM sequence or artificial miRNA under CaMV 35S promoter
    Depositor
    Guiliang Tang
    Type
    Empty backbone
    Use
    RNAi and Synthetic Biology
    Expression
    Plant
    Promoter
    CaMV 35S promoter
    Available since
    Oct. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    141177-plasmid-map-sequence-id-299765

    Enlarge

  15. pUC-L1L4

    Plasmid
    #114018
    Purpose
    Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning
    Depositor
    Christopher Grefen
    Insert
    ccdB gene, Chloramphenicol resistance gene
    Use
    Gateway Cloning
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    114018-plasmid-map-sequence-id-517655

    Enlarge

  16. pREVtre GFP

    Plasmid
    #128069
    Purpose
    Retroviral tet inducible EGFP
    Depositor
    Oskar Laur
    Insert
    tet inducible EGFP
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    no
    Available since
    Aug. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    128069-plasmid-map-sequence-id-255185

    Enlarge

  17. FUGW ds red

    Plasmid
    #128067
    Purpose
    Lentiviral expression of dsRed Express2
    Depositor
    Oskar Laur
    Insert
    ds red
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    no
    Available since
    Aug. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    128067-plasmid-map-sequence-id-255182

    Enlarge

  18. pCoofy42

    Plasmid
    #55190
    Purpose
    Pichia pastoris expression vector for parallel SLIC cloning. C-terminal tags can be added during SLIC.
    Depositor
    Sabine Suppmann
    Publication
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Tags
    His10-Stop-OneStrep-Stop-S-tag-Stop-CBP-HPC4-Stop…
    Expression
    Yeast
    Promoter
    AOX1
    Available since
    July 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    55190-plasmid-map-sequence-id-85572

    Enlarge

  19. pCMVlac-dirTopo-AU1-aGFP

    Plasmid
    #61971
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    61971-plasmid-map-sequence-id-101286

    Enlarge

  20. pCMVlac-dirTopo-P2A-aGFP

    Plasmid
    #61972
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    61972-plasmid-map-sequence-id-101285

    Enlarge

Showing: 5841 - 5860 of 63044 results