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pMBA329

Plasmid

#214749

Purpose

High copy glmS-nadE plasmid containing mCherry and no antibiotic resistance gene (ARG)

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23119-RBS(22821)-mcherry

Expression

Bacterial

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pMBA327

Plasmid

#214747

Purpose

Low copy glmS-nadE plasmid containing mCherry and no antibiotic resistance gene (ARG)

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23119-RBS(22821)-mcherry

Expression

Bacterial

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pMBA325

Plasmid

#214742

Purpose

Medium copy glmS-nadE plasmid containing spectinomycin as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(8455)-glmS-RBS(5392)-nadE

Expression

Bacterial

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMBA324

Plasmid

#214741

Purpose

Low copy glmS-nadE plasmid containing kanamycin as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(8455)-glmS-RBS(5392)-nadE

Expression

Bacterial

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMBA326

Plasmid

#214743

Purpose

High copy glmS-nadE plasmid containing chloramphenicol as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(8455)-glmS-RBS(5392)-nadE

Expression

Bacterial

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pSV014

Plasmid

#214740

Purpose

High copy thyA-infA plasmid containing chloramphenicol as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(7504)-thyA-RBS(4427)-infA

Expression

Bacterial

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pSV013

Plasmid

#214739

Purpose

Medium copy thyA-infA plasmid containing spectinomycin as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(7504)-thyA-RBS(4427)-infA

Expression

Bacterial

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pSV015

Plasmid

#214738

Purpose

Low copy thyA-infA plasmid containing kanamycin as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(7504)-thyA-RBS(4427)-infA

Expression

Bacterial

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pMAL-ccDockerin

Plasmid

#72200

Purpose

MBP-ccDockerin expression plasmid

Depositor

Timothy Whitehead

Article

Kowalsky et al Proteins. 2016 Dec;84(12):1914-1928. doi

Insert

Clostridium Cellulolyticum Type I Dockerin

Tags

His-Tag and Maltose Binding Protein

Expression

Bacterial

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pET24_BD3179

Plasmid

#73142

Purpose

Expresses in E.coli B. bacteriovorus Bd3179

Depositor

Sandra Gabelli

Article

de la Peña et al PLoS One. 2015 Nov 2;10(11):e0141716. do

Insert

Bd3179

Expression

Bacterial

Available Since

March 22, 2016

Availability

Academic Institutions and Nonprofits only
mPapaya-Cx43-7

Plasmid

#56642

Purpose

Localization: Gap Junctions, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Cx43

Tags

mPapaya

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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mPapaya1-LC-Myosin-C-10

Plasmid

#56402

Purpose

Localization: Light Chain Myosin, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

LC-Myosin

Tags

mPapaya1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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mPapaya1-MannII-N-10

Plasmid

#56354

Purpose

Localization: Mannosidase/Golgi, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

MannII

Tags

mPapaya1

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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mPapaya-MannII-N-10

Plasmid

#56347

Purpose

Localization: Mannosidase/Golgi, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

MannII

Tags

mPapaya

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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HA-Rab11-WT

Plasmid

#101047

Purpose

Expresses HA-tagged human Rab11-WT in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

RAB11 (RAB11A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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Cry2 mCherry RGS4(del 1-33) in pcDNA3.1

Plasmid

#64207

Purpose

mCherry fused between Cry2 and RGS4(del 1-33) to study cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Inserts

Cry2 (CRY2 Mustard Weed)
RGS4(del 1-33) (RGS4 Human)

Tags

mcherry

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
pLV-CMV-LoxP-DsRed-LoxP-eGFP

Plasmid

#65726

Purpose

Functions as a genetic Cre reporter that switches from DsRed expression to eGFP expression upon presence of Cre recombinase.

Depositor

Jacco van Rheenen

Article

Zomer et al Cell. 2015 May 21;161(5):1046-57. doi: 1

Insert

LoxP-DsRed-LoxP-eGFP

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pB80-hKif5b(1-560)rigor-2xmNeongreen

Plasmid

#174649

Purpose

Live-cell marker for stable microtubules

Depositor

Lukas Kapitein

Article

Jansen et al J Cell Biol. 2023 May 1;222(5):e20210610

Insert

hKif5B(1-560)rigor-2xmNeongreen (KIF5B Human)

Tags

mNeongreen-mNeongreen

Expression

Mammalian

Mutation

rigor mutation G234A

Promoter

Chicken beta-actin

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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ABE8.20-m

Plasmid

#136300

Purpose

expresses ABE8.20-m in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.20-m

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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plentiCMV-sct-pp65-b2m-HLA-A0201

Plasmid

#196511

Purpose

Express single-chain peptide-MHC (pp65 CMV epitope on HLA-A0201)

Depositor

Howard Chang

Article

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

single chain of a pp65 peptide, b2m and HLA A0201

Use

Lentiviral

Tags

NA

Expression

Mammalian

Mutation

Y84C

Promoter

CMV

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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