We narrowed to 7,766 results for: loxP

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pCAS-mak10 gRNA-MAK10Δ

Plasmid

#160384

Purpose

For CRISPR-mediated deletin og MAK10 in yeast

Depositor

John McCusker

Insert

MAK10

Expression

Yeast

Promoter

PGK1

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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ACH1-2EGFP-ter-URA3

Plasmid

#184769

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at ACH1 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

ACH1 (ACH1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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SYS1-2EGFP-ter-URA3

Plasmid

#184771

Purpose

Marker for yeast early Golgi. Integration of 2xGFP tag at SYS1 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

SYS1 (SYS1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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SEC7-2Katushka-ter-Hygromycin

Plasmid

#184772

Purpose

Red marker for yeast late Golgi/early endosome. Integration of 2xKatushka tag at SEC7 C terminus. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

SEC7 (SEC7 Budding Yeast)

Tags

2Katushka

Expression

Yeast

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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UAS-GFP::NMN-Deamidase

Plasmid

#187868

Purpose

Gal4/UAS expression of GFP::NMN-Deamidase

Depositor

Lukas Neukomm

Publication

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

NMN-Deamidase

Tags

GFP

Expression

Insect

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4D41

Plasmid

#185841

Purpose

Testing the CYC1 core promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter

Depositor

Claudia Vickers

Publication

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PCYC1+[Z268]>yEGFP>TPGK1-TURA3

Expression

Yeast

Mutation

None

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4D42

Plasmid

#185840

Purpose

Testing the TEF1 promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter

Depositor

Claudia Vickers

Publication

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PTEF1+[Z268]>yEGFP>TPGK1-TURA3

Expression

Yeast

Mutation

None

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP10CF71

Plasmid

#185860

Purpose

Testing the SkGAL2 promoter inserted with 4 Z268 elements using a green fluorescent protein as the reporter

Depositor

Claudia Vickers

Publication

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1- PSkGAL2+[Z268]>(BamHI)yEGFP>TURA3

Expression

Yeast

Mutation

None

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP10CF72 (B9)

Plasmid

#185858

Purpose

Testing the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporter

Depositor

Claudia Vickers

Publication

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1- PSkGAL2M5(5*PZ4)>(BamHI)yEGFP>TURA3

Expression

Yeast

Mutation

None

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP10CF72

Plasmid

#185857

Purpose

Testing the SkGAL2 promoter inserted with 4 PZ4 elements using a green fluorescent protein as the reporter

Depositor

Claudia Vickers

Publication

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1- PSkGAL2M4(4*PZ4)>(BamHI)yEGFP>TURA3

Expression

Yeast

Mutation

None

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP10E1B

Plasmid

#185856

Purpose

Testing the SkGAL2 promoter inserted with 3 PZ4 elements using a green fluorescent protein as the reporter

Depositor

Claudia Vickers

Publication

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1- PSkGAL2M2(3*PZ4)>(BamHI)yEGFP>TURA3

Expression

Yeast

Mutation

None

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP10E1A

Plasmid

#185855

Purpose

Testing the SkGAL2 promoter inserted with 2 PZ4 elements using a green fluorescent protein as the reporter

Depositor

Claudia Vickers

Publication

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1- PSkGAL2M1(2*PZ4)> (BamHI)yEGFP>TURA3

Expression

Yeast

Mutation

None

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pNUT2

Plasmid

#175745

Purpose

Single-component photo-inducible (LOV2-based) nucleolus-targeting tool 2

Depositor

Yubin Zhou

Publication

Tan et al Nucleic Acids Res. 2022 Mar 23. pii: 655

Insert

mCherry-LOV2-NoLS (human nuclear factor-κB inducing kinase)

Tags

mCherry

Expression

Mammalian

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD6-GCaMP6f

Plasmid

#178729

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of GCaMP6f in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Publication

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GCaMP6f

Use

AAV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD6-GFP

Plasmid

#178711

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of GFP in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Publication

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GFP

Use

AAV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pYL237

Plasmid

#173194

Purpose

CRISPR donor plasmid for making the RNA-binding mutant EZH2 control cell line. It contains a puromycin resistance gene and an mCherry gene

Depositor

Thomas Cech

Publication

Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

Insert

EZH2 RNA-binding mutant

Use

CRISPR

Expression

Mammalian

Available since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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p{Mhc-F4-678-GFP::KD-RC,attB}

Plasmid

#182184

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Expression

Insect

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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p{Mhc-F4-678-DsRed::KD-RC,attB}

Plasmid

#182189

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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p{Mhc-F4-678-eGFP::KD,attB}

Plasmid

#182183

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Expression

Insect

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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p{Mhc-F4-678-DsRed::KD,attB}

Plasmid

#182188

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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