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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,887 results for: Coli

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Showing: 5901 - 5920 of 9887 results

  1. pHL 2057

    Plasmid
    #64909
    Purpose
    AmpR + ColE1 + PLtetO-1::RBS(st7):cro::cII (31 basepairs)::T1 terminator + PLlacO-1::RBS(st7):psu::T1 terminator
    Depositor
    Han Lim
    Article
    Hussein et al Biochim Biophys Acta. 2015 May 14;1849(8
    Type
    Empty backbone
    Use
    Synthetic Biology; E. coli
    Expression
    Bacterial
    Available Since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. pHL 2067

    Plasmid
    #64910
    Purpose
    AmpR + ColE1 + PLtetO-1:: extra 5ā€™UTR::RBS(st7):cfp::T1 terminator + PLlacO-1::RBS(st7):psu::T1 terminator
    Depositor
    Han Lim
    Article
    Hussein et al Biochim Biophys Acta. 2015 May 14;1849(8
    Type
    Empty backbone
    Use
    Synthetic Biology; E. coli
    Expression
    Bacterial
    Available Since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. I766T USP7 UBL-12

    Plasmid
    #227232
    Purpose
    Expresses human UBL-12 domain of USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    I766T; aa 535-775
    Promoter
    T7-promoter
    Available Since
    April 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    227232-plasmid-map-sequence-id-468099

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  9. L757P USP7 UBL-12

    Plasmid
    #227231
    Purpose
    Expresses human UBL-12 domain of USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    L757P; aa 535-775
    Promoter
    T7-promoter
    Available Since
    April 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    227231-plasmid-map-sequence-id-468098

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  12. R152C USP7 TRAF

    Plasmid
    #227229
    Purpose
    Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    R152C; aa 62-205
    Promoter
    T7-promoter
    Available Since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    227229-plasmid-map-sequence-id-452330

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  13. G392A FL USP7

    Plasmid
    #227217
    Purpose
    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    G392A
    Promoter
    T7-promoter
    Available Since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    227217-plasmid-map-sequence-id-452329

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  14. L469R USP7 catalytic

    Plasmid
    #227212
    Purpose
    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    L469R; aa 207-560
    Promoter
    T7-promoter
    Available Since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    227212-plasmid-map-sequence-id-452328

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  15. pET27b+R

    Plasmid
    #188245
    Purpose
    Empty backbone vector for protein expression in Escherichia coli and his-tag purification
    Depositor
    Yiping Qi
    Article
    Fang et al Methods Mol Biol. 2023;2653:153-171. doi
    Type
    Empty backbone
    Tags
    6xHis tag and TEV site
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    Oct. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    188245-plasmid-map-sequence-id-368371

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  17. pBz84

    Plasmid
    #226318
    Purpose
    E. coli protein expression for protein purification
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    6xHis- (wtMR1-6)- strep
    Tags
    6xHis and Strep
    Expression
    Bacterial
    Mutation
    N-terminal domain deleted, C-terminal domain deleā€¦
    Promoter
    T7
    Available Since
    Oct. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    226318-plasmid-map-sequence-id-449972

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  18. N418S FL USP7

    Plasmid
    #227215
    Purpose
    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    N418S
    Promoter
    T7-promoter
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227215-plasmid-map-sequence-id-449605

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  19. H111Q USP7 TRAF

    Plasmid
    #227227
    Purpose
    Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    H111Q; aa 62-205
    Promoter
    T7-promoter
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227227-plasmid-map-sequence-id-449602

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  20. G392D FL USP7

    Plasmid
    #227216
    Purpose
    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    G392D
    Promoter
    T7-promoter
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227216-plasmid-map-sequence-id-449551

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Showing: 5901 - 5920 of 9887 results