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pcDNA5-FRT/TO-3*Flag-APEX2 N-term NONO

Plasmid

#187580

Purpose

Express FLAG epitope and APEX2-tagged NONO fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

NONO (NONO Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag FKHR H215R AAA mutant

Plasmid

#13510

Depositor

Kunliang Guan

Article

Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

Insert

FKHR H215R AAA (FOXO1 Human)

Tags

Flag

Expression

Mammalian

Mutation

H215R T24A S256A S319A

Available Since

Dec. 11, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO-Venus-Flag-Gateway (1124)

Plasmid

#40999

Depositor

Jonathon Pines

Article

Di Fiore et al J Cell Biol. 2010 Aug 23;190(4):501-9.

Type

Empty backbone

Tags

3xFLAG (DYKDHDGDYKDHDIDYKDDDDK) and Venus YFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1alpha-IRES-EGFP-Flag-hRPGRIP1L

Plasmid

#122991

Purpose

Expresses Flag-tagged hRPGRIP1L in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Insert

RPGRIP1L (RPGRIP1L Human)

Use

Lentiviral

Tags

Flag

Promoter

EF1 alpha

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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PB-EF1-6xflag-RT3-3xHaloTag(MtoL)-H2B

Plasmid

#106309

Purpose

The plasmid utilizes RT3 sequence to minimize the expression of 3xHaloTag (Methionine mutated to Leucine)-H2B for long-term H2B single-molecule imaging.

Depositor

Zhe J. Liu

Article

Liu et al Proc Natl Acad Sci U S A. 2017 Dec 28. p

Insert

6xflag-RT3-3xHaloTag(MtoL)-H2B (H2BC3 Human)

Tags

6xflag-3xHaloTag(MtoL)

Expression

Mammalian

Promoter

EF1

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pET Flag TEV LIC cloning vector (1L)

Plasmid

#29662

Depositor

Scott Gradia

Type

Empty backbone

Tags

Flag and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CALR del52-FLAG

Plasmid

#204523

Purpose

Retroviral expression of human CALR del52-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR del52-FLAG (CALR Human)

Use

Retroviral

Mutation

del52

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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EK0397 3FLAG-ADAR1-p150-HIS (pEAQ)

Plasmid

#191172

Purpose

Human ADAR1, p150 isoform in plant-expressable format.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

3FLAG-ADAR1-p150-HIS (plant) (ADAR Human)

Tags

3FLAG and HIS

Expression

Plant

Mutation

WT

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pKS021 - pCAGGS-3XFLAG-mKate2-SA1-bpA

Plasmid

#156440

Purpose

For transient expression of mouse SA1 (= STAG1 or SCC3A) tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse SA1 (Stag1 Mouse)

Expression

Mammalian

Mutation

none

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-flag-myr-Akt1-IRES-GFP

Plasmid

#65063

Purpose

mammalian retroviral expression plasmid

Depositor

Kira Gritsman, Jean Zhao

Article

Kharas et al Blood. 2010 Feb 18;115(7):1406-15. doi:

Insert

Akt1 (AKT1 Human)

Tags

flag and myristoylation tag

Expression

Mammalian

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT/TO-3*Flag-APEX2 N-term COILIN

Plasmid

#187581

Purpose

Express FLAG epitope and APEX2-tagged COILIN fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

COILIN (COIL Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-CDK8-W105M-IRES-mCherry

Plasmid

#68857

Purpose

Expresses Human CDK8 W105M Mutant

Depositor

Matthew Shair

Article

Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

Insert

Cyclin-Dependent Kinase 8 (CDK8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

W105M

Promoter

EF1α

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-FLAG-TurboID-dPB1-SQSTM1

Plasmid

#223716

Purpose

Expresses FLAG-TurboID-dPB1-SQSTM1

Depositor

Benjamin Wolozin

Article

Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

Insert

SQSTM1 (SQSTM1 Human)

Use

Lentiviral

Tags

TurboID

Expression

Mammalian

Mutation

deletion 1-119

Promoter

SFFV

Available Since

Aug. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHR-SFFV-FLAG-TurboID-dUBA-SQSTM1

Plasmid

#223717

Purpose

Expresses FLAG-TurboID-dUBA-SQSTM1

Depositor

Benjamin Wolozin

Article

Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

Insert

SQSTM1 (SQSTM1 Human)

Use

Lentiviral

Tags

TurboID

Expression

Mammalian

Mutation

deletion 384-Stop codon

Promoter

SFFV

Available Since

Aug. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A

Plasmid

#51248

Purpose

Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli3 (Gli3 Mouse)

Use

Flpin system

Tags

3xHA-TEV and Flag

Expression

Mammalian

Mutation

amino acids 849,865,877,907,980,1006 mutated to A…

Promoter

EF1a

Available Since

March 7, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA5-FRT/TO-NLS-CRBNmidi-miniTurbo-3xFLAG

Plasmid

#248675

Purpose

To generate inducible Flp-In cells expressing Cterm tagged miniTurbo CRBNmidi for ProtacID.

Depositor

Cheryl Arrowsmith

Article

Shrestha et al Nat Commun. 2025 Aug 29;16(1):8089. doi:

Insert

CRBNmidi

Tags

NLS and miniTurbo-3x FLAG

Expression

Mammalian

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pCDNA5-FRT/TO-NLS-VHL-miniTurbo-3xFLAG

Plasmid

#248670

Purpose

To generate inducible Flp-In cells expressing Cterm tagged miniTurbo full length VHL for ProtacID.

Depositor

Cheryl Arrowsmith

Article

Shrestha et al Nat Commun. 2025 Aug 29;16(1):8089. doi:

Insert

VHL (VHL Human)

Tags

FLAG-miniTurbo and NLS

Expression

Mammalian

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-2xFLAG-2xSTREP-CCND3-IRES-mCherry

Plasmid

#172620

Purpose

Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D3 and mCherry in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND3 (CCND3 Human)

Use

Lentiviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pTRIPdU3-MND-FLAG-FLI1-IRES-GFP

Plasmid

#129496

Purpose

Lentiviral expression of human FLI1

Depositor

Marjorie Brand

Article

Palii et al Cell Stem Cell. 2019 May 2;24(5):812-820

Insert

FLI1 (FLI1 Human)

Use

Lentiviral

Tags

FLAG

Promoter

MND

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-UBC mTagBFP2-P2A-3xFLAG-hEEA1

Plasmid

#214995

Purpose

Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mTagBFP2 to sort for even expression levels.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

EEA1 (EEA1 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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