We narrowed to 17,340 results for: emb

1. iRFP-PAmCherry1-PH_PLCd1

   Plasmid

   #92167

   Purpose

   PI(4,5)P2 single molecule fluorescent reporter

   Depositor

   Xuelin Lou

   Article

   Ji et al J Biol Chem. 2015 Nov 6;290(45):26978-93

   Insert

   iRFP-PAmCherry1-PH_PLCd1
   

   Tags

   iRFP-PAmCherry1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLCS108

   Plasmid

   #209329

   Purpose

   Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mCherry fusion protein.

   Depositor

   Lucia Strader

   Article

   Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDD363

   Plasmid

   #91829

   Purpose

   LoxP-flanked SEC donor for the SapTrap cloning system

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   LoxP-SEC-LoxP
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDD379

   Plasmid

   #91834

   Purpose

   SapTrap destination vector for building combined sgRNA expression + repair template vectors, using the F+E sgRNA scaffold

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Worm

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-MBP1-E322TAG-W340A.pcDNA3-k

   Plasmid

   #127404

   Purpose

   Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site.

   Depositor

   Sharona Gordon

   Article

   Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

   Insert

   Maltose Binding Protein
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Amino acid mutations: E322 to Amber codon and W34…

   Promoter

   CMV

   Available Since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-N-Zeo-mScarlet

   Plasmid

   #207700

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-mScarlet cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. Cdk9-Halo donor

   Plasmid

   #113113

   Purpose

   Donor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Cdk9 coding sequence

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   Cdk9 donor region including homology arms and HaloTag
   

   Use

   Synthetic Biology

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. hRIP3 GFP D160N

   Plasmid

   #41386

   Depositor

   Francis Chan

   Article

   Cho et al Cell. 2009 Jun 12;137(6):1112-23.

   Insert

   RIP3 (RIPK3 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Kinase Domain Mutant (D160N)

   Promoter

   CMV

   Available Since

   Dec. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFUS_B6

   Plasmid

   #31023

   Depositor

   Adam Bogdanove, Daniel Voytas

   Article

   Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

   Insert

   LacZ + BsaI restriction sites
   

   Available Since

   June 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFUS_B5

    Plasmid

    #31022

    Depositor

    Adam Bogdanove, Daniel Voytas

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    LacZ + BsaI restriction sites
    

    Available Since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLCS104

    Plasmid

    #209357

    Purpose

    Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal HaloTag fusion protein.

    Depositor

    Lucia Strader

    Article

    Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Human p150,95

    Plasmid

    #8633

    Depositor

    Timothy Springer

    Article

    Corbi et al EMBO J 1987 Dec 20;6(13):4023-8.

    Insert

    Human p150,95 (ITGAX Human)
    

    Expression

    Mammalian

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. CycA-Venus-Flag (1305)

    Plasmid

    #39853

    Depositor

    Jonathon Pines

    Article

    Di Fiore et al J Cell Biol. 2010 Aug 23;190(4):501-9.

    Insert

    Cyclin A (CCNA2 Human)
    

    Tags

    Flag and Venus

    Expression

    Mammalian

    Available Since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pFUS_B3

    Plasmid

    #31020

    Depositor

    Adam Bogdanove, Daniel Voytas

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    LacZ + BsaI restriction sites
    

    Available Since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-6P-1 Stu2 1-306

    Plasmid

    #38315

    Depositor

    Anthony Hyman

    Article

    Widlund et al Mol Biol Cell. 2012 Sep 19.

    Insert

    5' 918 bp from STU2 ORF (STU2 Budding Yeast)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOGG005

    Plasmid

    #113980

    Purpose

    Level 1 high copy number vector for E. coli expression

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Type

    Empty backbone

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFUS_B1

    Plasmid

    #31018

    Depositor

    Adam Bogdanove, Daniel Voytas

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    LacZ + BsaI restriction sites
    

    Available Since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEST14-DoubleCatcher-⍺-Lock

    Plasmid

    #216286

    Purpose

    Expresses DoubleCatcher-⍺-Lock in bacterial cells. DoubleCatcher-⍺-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher ⍺-Lock
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST14-DoubleCatcher-β-Lock

    Plasmid

    #216287

    Purpose

    Expresses DoubleCatcher-β-Lock in bacterial cells. DoubleCatcher-β-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher β-Lock
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDEST14-DoubleCatcher-γ-Lock

    Plasmid

    #216288

    Purpose

    Expresses DoubleCatcher-γ-Lock in bacterial cells. DoubleCatcher-γ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher γ-Lock
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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