We narrowed to 10,183 results for: yeast

1. pILGFP5EC4

   Plasmid

   #185880

   Purpose

   A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (9 copy)

   Depositor

   Claudia Vickers

   Type

   Empty backbone

   Expression

   Yeast

   Mutation

   None

   Available Since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pILGFP5EF3

   Plasmid

   #185881

   Purpose

   A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (13 copy)

   Depositor

   Claudia Vickers

   Type

   Empty backbone

   Expression

   Yeast

   Mutation

   None

   Available Since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pILGFP3AE4

   Plasmid

   #185873

   Purpose

   A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~11 copy)

   Depositor

   Claudia Vickers

   Type

   Empty backbone

   Expression

   Yeast

   Mutation

   None

   Available Since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pREP41-MCS+

   Plasmid

   #52690

   Purpose

   Same as pREP41, with better MCS

   Depositor

   Michael Nick Boddy

   Type

   Empty backbone

   Expression

   Yeast

   Promoter

   Pnmt41

   Available Since

   April 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHS12-mCherry

   Plasmid

   #25444

   Depositor

   Benjamin Glick

   Insert

   mCherry
   

   Tags

   COX4 Presequence

   Expression

   Yeast

   Available Since

   June 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pREP42-MCS+

   Plasmid

   #52691

   Purpose

   Same as pREP42, with better MCS

   Depositor

   Michael Nick Boddy

   Type

   Empty backbone

   Expression

   Yeast

   Promoter

   Pnmt41

   Available Since

   April 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pILGFP5EG3

   Plasmid

   #185878

   Purpose

   A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (1 copy reference)

   Depositor

   Claudia Vickers

   Type

   Empty backbone

   Expression

   Yeast

   Mutation

   None

   Available Since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pILGFP3A5C

   Plasmid

   #185872

   Purpose

   A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (1 copy reference)

   Depositor

   Claudia Vickers

   Type

   Empty backbone

   Expression

   Yeast

   Mutation

   None

   Available Since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGP6G-ccdB

   Plasmid

   #83880

   Purpose

   Destination vector for integration at yeast URA3 locus, expression from the GPD promoter

   Depositor

   Jennifer Nemhauser

   Type

   Empty backbone

   Use

   Gateway destination vector

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP4G-cccdB

    Plasmid

    #83876

    Purpose

    Destination vector for integration at yeast Trp locus, expression from the GPD promoter

    Depositor

    Jennifer Nemhauser

    Type

    Empty backbone

    Use

    Gateway destination vector

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGP5G-ccdB

    Plasmid

    #83879

    Purpose

    Destination vector for integration at yeast Leu locus, expression from the GPD promoter

    Depositor

    Jennifer Nemhauser

    Type

    Empty backbone

    Use

    Gateway destination vector

    Available Since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRCT

    Plasmid

    #60621

    Purpose

    Plasmid encoding iCas9, tracrRNA and crRNAs

    Depositor

    Huimin Zhao

    Article

    Bao et al ACS Synth Biol. 2014 Sep 19.

    Inserts

    iCas9
    tracrRNA
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Yeast

    Mutation

    changed Aspartate 147 to Tyrosine, Proline 411 to…

    Promoter

    RPR1p and TEF1p

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAP01

    Plasmid

    #59981

    Purpose

    The function of this material is to facilitate the capture and expression of bacterial gene clusters.

    Depositor

    Bradley Moore

    Article

    Yamanaka et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

    Type

    Empty backbone

    Use

    Synthetic Biology; Transformation-associated reco…

    Tags

    no

    Expression

    Bacterial and Yeast

    Promoter

    no

    Available Since

    Nov. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPRi_Mxi1_yl_NHEJ

    Plasmid

    #91249

    Purpose

    CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

    Inserts

    Codon optimized dCas9-Mxi1
    KU70 sgRNA expression cassette
    KU80a sgRNA expression cassette
    KU80b sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. BB1_12_pAOX1

    Plasmid

    #98514

    Purpose

    BB1 with insert: promoter PAOX1

    Depositor

    Michael Sauer, Diethard Mattanovich, Brigitte Gasser

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Insert

    AOX1 promoter
    

    Expression

    Bacterial

    Mutation

    Internal BsaI and BpiI sites removed from insert

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. HC078 (3xHA-Atg13)

    Plasmid

    #59544

    Purpose

    Expresses yeast Atg13 under the endogenous promoter with a 3xHA N-terminal tag

    Depositor

    Daniel Klionsky

    Article

    Cheong et al Mol Biol Cell. 2008 Feb;19(2):668-81. Ep

    Insert

    ATG13 (ATG13 Budding Yeast)
    

    Tags

    3xHA

    Expression

    Bacterial and Yeast

    Mutation

    AvrII site created after start codon

    Promoter

    ATG13

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pfa6a mScarlet-FKBP::KANMX

    Plasmid

    #185905

    Purpose

    Plasmid for cloning mScarlet fused to FKBP into yeast.

    Depositor

    Matthew Good

    Article

    Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

    Insert

    mScarlet-FKBP
    

    Tags

    mScarlet fused to FKBP

    Expression

    Yeast

    Promoter

    SP6

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRISPRi_Mxi1_yl

    Plasmid

    #91248

    Purpose

    CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

    Inserts

    Codon optimized dCas9-Mxi1
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEVA1

    Plasmid

    #58953

    Purpose

    Contains a synthetic DNA sequence, VAR1u, that specifies the Var1 protein. The Cox4 targeting signal is fused to the N-terminus to direct import of Var1. Complements var1 mutations in mtDNA.

    Depositor

    Thomas Fox, Thomas Mason

    Article

    Sanchirico et al EMBO J. 1998 Oct 1;17(19):5796-804.

    Insert

    VAR1u, VAR1 recoded for expression from the nucleus
    

    Use

    Yeast ecoli shuttle vector

    Tags

    preCox4 mitochondrial matrix targeting signal

    Expression

    Yeast

    Mutation

    Synthetic gene in standard genetic code

    Promoter

    ADH1

    Available Since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

    Plasmid

    #78231

    Purpose

    Expresses Cas9 protein, under the control of the TEF1 promoter, and CYC terminator. KanMX resistance

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    Cas9
    

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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