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pJH1922

Plasmid

#132361

Purpose

Prgef-1 INS-1::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-1::GFP

Depositor

Mei Zhen

Publication

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

INS-1 (ins-1 Nematode)

Tags

GFP

Expression

Worm

Promoter

Prgef-1

Available since

Oct. 28, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pJH2255

Plasmid

#132364

Purpose

Prgef-1 INS-4::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-4::GFP

Depositor

Mei Zhen

Publication

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

INS-4 (ins-4 Nematode)

Tags

GFP

Expression

Worm

Promoter

Prgef-1

Available since

Oct. 7, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pJH2947

Plasmid

#132362

Purpose

Prgef-1 INS-2::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-2::GFP

Depositor

Mei Zhen

Publication

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

INS-2 (ins-2 Nematode)

Tags

GFP

Expression

Worm

Promoter

Prgef-1

Available since

Oct. 7, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pBT3047

Plasmid

#122547

Purpose

Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a N-terminal (sv-40) and C-terminal (egl-13) nuclear localization signal under the intestinal specific promoter nhx-2

Depositor

Gary Silverman

Publication

Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

Insert

Pnhx-2_NLS(sv-40)::CemOrange2::NLS(egl-13)

Tags

MAPKKKRKVV and MSRRRKANPTKLSENAKKLAKEVEN

Expression

Bacterial

Promoter

Pnhx-2

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEL392

Plasmid

#52348

Purpose

GFP reporter for fli-1

Depositor

Erik Lundquist

Publication

Lu et al BMC Dev Biol. 2008 May 16;8:54. doi: 10.

Insert

fli-1 (fli-1 Nematode)

Tags

GFP

Expression

Worm

Promoter

fli-1

Available since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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pGFPmAID_htz-1

Plasmid

#174214

Purpose

Donor plasmid for tagging htz-1 with GFP::mAID

Depositor

Masato Kanemaki

Publication

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Insert

GFP::mAID::htz-1 tagging donor

Use

CRISPR

Expression

Worm

Available since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pJH3043

Plasmid

#175284

Purpose

Pdpy-30 DAF-16a unc-54 3' UTR C.elegans pan-tissue expression of isoform a of DAF-16 fused to GFP

Depositor

Mei Zhen

Publication

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

daf-16a mini-gene (daf-16 Nematode)

Expression

Worm

Promoter

Pdpy-30

Available since

Nov. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pJW1882

Plasmid

#154330

Purpose

sgRNA Target Vector for CRISPR

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

ttTi4348 site targeting sgRNA (F+E) with R07E5.16 U6 promoter and 3'UTR

Use

CRISPR

Expression

Worm

Mutation

F+E mutations in sgRNA

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCZGY553

Plasmid

#110883

Purpose

Pmec-4 Gateway Destination vector for cloning for expression in C. elegans mechanosensory neurons

Depositor

Andrew Chisholm

Publication

Ghosh-Roy et al J Neurosci. 2010 Mar 3;30(9):3175-83. do

Insert

Pmec-4

Use

Gateway Cloning

Available since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1788

Plasmid

#154317

Purpose

CT Donor-cassette for SapTrap

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

linker::TEV::BioTag::AID*::3xFLAG donor for CT slot in the SapTrap cloning system

Use

CRISPR

Expression

Worm

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1787

Plasmid

#154316

Purpose

NT Donor-cassette for SapTrap

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

linker::TEV::BioTag::AID*::3xFLAG donor for NT slot in the SapTrap cloning system

Use

CRISPR

Expression

Worm

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1816

Plasmid

#154320

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::mScarlet-I^SEC (Lox511I)^3xMyc

Use

CRISPR and Cre/Lox

Expression

Worm

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1851

Plasmid

#154329

Purpose

sgRNA Target Vector for CRISPR

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

cxTi10882 site targeting sgRNA (F+E) with K09B11.2 U6 promoter and 3'UTR

Use

CRISPR

Expression

Worm

Mutation

F+E mutations in sgRNA

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pSDH50

Plasmid

#139037

Purpose

MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

Depositor

Erik Griffin

Publication

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

tomm-20 (1-55 a.a.) (no STOP codon) (tomm-20 Nematode)

Expression

Worm

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pAP639-2F

Plasmid

#99486

Purpose

3XFlag::TEV::6XHis::mTurquoise::6XHis::TEV::3XFlag

Depositor

Geraldine Seydoux

Publication

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::TEV::6XHis::mTurquoise::6XHis::TEV::3XFlag

Expression

Bacterial

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAP607-2

Plasmid

#99483

Purpose

Myc::TEV::3XFlag::GFP::3XFlag::TEV::Myc

Depositor

Geraldine Seydoux

Publication

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

Myc::TEV::3XFlag::GFP::3XFlag::TEV::Myc

Expression

Bacterial

Available since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pGC78

Plasmid

#19654

Depositor

Jane Hubbard

Publication

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

<1xNLS-lacZ::unc-54(3’)

Use

Flp/frt

Tags

1xNLS

Expression

Worm

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pOX-twk8

Plasmid

#16108

Depositor

Larry Salkoff

Publication

Wei et al Neuropharmacology. 1996 . 35(7):805-29.

Insert

Tandem pore domain K+ channel (twk-8 Nematode)

Use

Xenopus oocyte expression

Available since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pOX-twk42

Plasmid

#16212

Depositor

Larry Salkoff

Publication

Wei et al Neuropharmacology. 1996 . 35(7):805-29.

Insert

Tandem pore domain K+ channel (twk-42 Nematode)

Use

Xenopus oocyte expression

Mutation

N/A

Available since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pCFJ2262A - [1-2] ENTR - Fluor - ce-mNeon (1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159851

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-mNeon (1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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